FastQCFastQC Report
Mon 6 Jun 2016
ERR522859_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522859_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9742340
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289310.2969615102737125No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT255360.26211361952056694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230600.23669878078572498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147290.15118544415407387No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG147850.051.36652
ACCTGGG167500.044.386213
GTACCTG182400.042.2556841
CCTGGGG155850.032.4357344
ATAACGC11100.028.3846043
TATAACG9800.028.3109652
GTACATG331350.027.277051
TACATGG337050.026.3970622
TAACGCA11050.026.3846594
ACATGGG336250.025.6069033
CATGGGG210550.024.9917644
CTGGGGG105300.022.7301645
ATGGGGG133950.022.572595
TGGGGGG142150.021.1300146
TACCGGG13200.019.5937482
GAGTACT263250.018.94209912-13
GTATCAA559050.018.337361
GTATAAC25850.018.1915821
AGAGTAC412450.017.90030110-11
GTACCGG17000.017.7036191
Produced by FastQC (version 0.11.5)