FastQCFastQC Report
Mon 6 Jun 2016
ERR522859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9742340
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277400.28473652120537774No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT260490.26737929491272117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235290.24151281930213891No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147710.1516165520809169No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG148450.050.4754032
ACCTGGG169400.043.4258233
GTACCTG179450.042.285831
TATAACG10750.030.6098522
CCTGGGG153750.030.5418474
GTACATG336950.026.6808221
TAACGCA11550.026.4530834
ATAACGC12250.026.0927563
TACATGG344300.025.76362
CATGGGG211300.025.67154
ACATGGG338650.025.2481043
ATGGGGG130900.023.8802055
TACCGGG11950.023.2089652
CTGGGGG99050.021.5935
TGGGGGG133500.021.1932586
GTACCGG17400.020.8183081
GTATAAC27350.019.9528371
GAGTACT271900.018.84062612-13
AGAGTAC425350.017.5739210-11
AGTACTT290600.017.55546212-13
Produced by FastQC (version 0.11.5)