FastQCFastQC Report
Mon 6 Jun 2016
ERR522858_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522858_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8033184
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT447970.557649370411533No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245020.3050098192696694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195060.24281779179961518No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT134370.16726866955867065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109410.13619755255201424No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC95100.11838394340276534No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG278900.026.1634041
TACATGG288900.024.767392
ACATGGG292350.023.752213
CATGGGG205100.021.1547764
GAGTACT214200.021.078212-13
AGAGTAC345600.019.4182610-11
GTACTTT235350.018.93492514-15
AGTACTT226450.018.2470712-13
ATGGGGG114850.017.8876235
GAGTACA220650.017.7071291
TATACCG6550.017.2255135
TACCTGG59000.016.8129352
AGTACAT217200.016.6015512
GTATATA39950.015.8879581
ACTTTTT289900.015.75969916-17
CATGGGA104900.015.6394814
TACTTTT271450.015.15328314-15
TATAGCG3807.8196535E-814.8456735
GTACCTG69350.014.7795481
GTATTAC17000.014.6581131
Produced by FastQC (version 0.11.5)