FastQCFastQC Report
Mon 6 Jun 2016
ERR522858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8033184
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT468630.5833676908184848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226700.28220441608209146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189940.23644422933671133No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT135860.16912347582228915No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC110100.13705648968080403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104780.13043395993419296No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC97860.12181969191792444No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA80380.10005995132191668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG279700.025.5915371
TACATGG284900.024.4831242
ACATGGG291900.023.087433
CATGGGG197300.021.2471644
GAGTACT220250.020.44748312-13
GTACTTT231450.019.2013214-15
AGAGTAC356200.018.74719210-11
AGTACTT232500.017.9143512-13
ATGGGGG106600.017.2375165
GAGTACA224100.016.4950961
ACTTTTT282800.015.7373816-17
CATGGGA109300.015.5220934
AGTACAT220050.015.4860972
TACTTTT269200.015.1904614-15
TATAACG6200.015.1622512
TATACTG33450.015.03295
TACCTGG57150.014.88633252
CGTATAC6250.014.2867723
TATACCG7050.013.9986225
ATGGGGA87950.013.9463235
Produced by FastQC (version 0.11.5)