FastQCFastQC Report
Mon 6 Jun 2016
ERR522857_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522857_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7780914
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT729910.9380774546537849No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253920.3263369830330987No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT219690.2823447219696812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187840.24141122752417005No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC159500.20498877124204176No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC144270.18541523527955714No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG119400.15345240931849394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112680.14481589180911136No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG277700.028.4074131
TACATGG282950.027.431012
ACATGGG288250.025.8840143
CATGGGG192750.024.2429374
TACCTGG60500.024.166262
ATGGGGG113100.022.1134385
TATCACG7600.021.0314752
TATAACG7450.020.1928732
GTACCTG76400.019.7527371
GAGTACT223350.019.4680212-13
AGAGTAC343250.018.92202810-11
ATAACGC8900.018.4885183
TAACGCA8600.018.0388434
GTACTTT244300.017.73156714-15
AGTACTT237450.017.50076112-13
CATGGGA103500.017.350674
GAGTACA210850.016.9232521
ACCTGGG86850.016.4023
AGTACAT207850.015.9230792
TGGGGGG120850.015.9007566
Produced by FastQC (version 0.11.5)