FastQCFastQC Report
Mon 6 Jun 2016
ERR522857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7780914
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT752700.9673670728143249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236920.3044886500480535No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT222120.2854677483904847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184450.23705441288774046No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC168470.21651697988179794No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC154070.1980101566474067No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG122190.15703810632015724No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA120080.15432634263789574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110510.14202701636337323No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC97920.12584639799386035No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG62000.027.6701532
GTACATG260150.025.2907161
TACATGG264750.024.321762
ACATGGG270200.023.1673433
GTACCTG79250.022.7876321
CATGGGG182750.021.4469744
GAGTACT224500.020.071312-13
ATGGGGG111600.020.0023575
ACCTGGG86400.019.4727213
AGAGTAC348500.019.01337610-11
GTACTTT239050.018.62085214-15
AGTACTT237000.018.31843212-13
GAGTACA207400.016.3490961
GTATAGG23750.016.2374381
GGGTTAG24150.015.7737561
ACTTTTT293600.015.30283116-17
TACTTTT279400.015.2671714-15
AGTACAT206400.014.9156432
GTATATA35300.014.7882071
CATGGGA96500.014.6100374
Produced by FastQC (version 0.11.5)