FastQCFastQC Report
Mon 6 Jun 2016
ERR522856_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522856_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7494852
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221280.2952426545580887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177640.23701602113023715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109950.14670069535729324No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131150.039.8170281
GTATCAA220000.037.4222221
GTACATG257800.030.2562981
TCAACGC268000.029.9147114
CAACGCA271050.029.5428265
ATCAACG274550.029.2879833
TACATGG268650.028.639752
TATCAAC281950.028.572362
AACGCAG282300.028.4933456
ACATGGG274550.027.2338873
CATGGGG195850.025.4584754
ACGCAGA319150.025.1741457
ATGGGGG121350.024.5516875
CGCAGAG332050.024.195178
TACCTGG59650.022.4564842
GCAGAGT371050.021.4614379
AGAGTAC308000.020.40189210-11
GAGTACT199450.020.00026912-13
CAGAGTA357500.018.15524910-11
AGTACTT216250.017.96848912-13
Produced by FastQC (version 0.11.5)