FastQCFastQC Report
Mon 6 Jun 2016
ERR522856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522856_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7494852
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195230.2604854638890801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171220.2284501415104661No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102610.1369073065085208No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153450.039.9755741
GTATCAA238400.032.952951
GTACATG252100.027.486151
TCAACGC286850.026.9169674
CAACGCA289900.026.5851465
ATCAACG290400.026.555913
TATCAAC298450.026.094622
TACATGG264000.025.9548112
AACGCAG299600.025.7869836
ACATGGG268750.024.8132213
CATGGGG189500.022.8150834
ACGCAGA340750.022.6725677
TACCTGG61850.022.430792
CGCAGAG353800.021.8797788
ATGGGGG116550.021.208845
GAGTACT205950.020.34555812-13
GCAGAGT393150.019.414769
AGAGTAC319900.019.085510-11
GTACCTG76100.018.5447371
GTACTTT223850.018.4757614-15
Produced by FastQC (version 0.11.5)