##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522856_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7494852
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.392346773491994	33.0	31.0	34.0	30.0	35.0
2	32.51229137012979	34.0	31.0	34.0	30.0	34.0
3	32.73495127055211	34.0	31.0	34.0	31.0	36.0
4	35.553703128494064	37.0	35.0	37.0	32.0	37.0
5	35.65959367843421	37.0	35.0	37.0	35.0	37.0
6	35.7460192676253	37.0	35.0	37.0	35.0	37.0
7	35.584628622419764	37.0	35.0	37.0	33.0	37.0
8	35.609842862807696	37.0	35.0	37.0	33.0	37.0
9	36.80893578685743	38.0	35.0	39.0	34.0	39.0
10-11	36.89103680766478	38.0	35.5	39.0	33.0	39.0
12-13	36.75796973709421	38.0	35.5	39.0	33.0	39.0
14-15	37.79482736950643	39.0	36.0	41.0	33.0	41.0
16-17	37.81388865317153	39.0	37.0	41.0	33.0	41.0
18-19	37.80989771379075	39.0	37.0	41.0	33.0	41.0
20-21	37.68844154627736	39.0	36.5	41.0	33.5	41.0
22-23	37.59736723286864	38.5	36.5	41.0	33.0	41.0
24-25	37.435754101615345	38.0	36.0	41.0	32.5	41.0
26-27	37.295487756129134	38.0	36.5	40.5	32.5	41.0
28-29	37.2560151287844	38.0	36.0	40.0	33.0	41.0
30-31	37.0874472237744	38.0	36.0	40.0	32.5	41.0
32-33	36.90838311416957	38.0	36.0	40.0	31.5	41.0
34-35	36.72035785363073	38.0	35.0	40.0	31.5	41.0
36-37	36.73259665434354	38.0	35.5	40.0	31.5	41.0
38-39	36.84858520221613	38.0	35.0	40.0	31.5	41.0
40-41	36.77176940918913	38.0	36.5	40.0	31.5	41.0
42-43	36.801885680998105	38.0	36.5	40.0	32.0	41.0
44-45	36.608559982238475	38.0	35.5	40.0	31.0	41.0
46-47	36.36152875333629	38.0	35.5	40.0	30.5	41.0
48-49	36.293136608968396	37.5	35.5	40.0	30.0	41.0
50-51	35.90756908875586	37.0	35.0	40.0	29.0	41.0
52-53	35.923148449095464	37.5	35.0	40.0	29.5	41.0
54-55	35.72920052323915	36.5	35.0	40.0	29.0	41.0
56-57	35.41324992141273	37.0	34.5	40.0	27.5	41.0
58-59	35.28551644515463	36.5	35.0	40.0	28.0	41.0
60-61	35.00115018949006	36.5	34.5	39.0	27.5	41.0
62-63	34.77402936041966	36.5	34.0	39.0	26.5	41.0
64-65	34.632723301274	37.0	34.0	39.0	26.0	40.5
66-67	34.3830772108642	36.5	34.0	38.5	26.0	40.0
68-69	33.92192480918903	35.5	33.0	37.5	25.5	40.0
70-71	33.64696240833041	36.0	33.0	37.5	25.0	39.5
72-73	33.39826523592461	35.5	33.0	37.0	25.0	39.0
74-75	33.0860154409987	35.0	32.0	37.0	24.0	39.0
76-77	31.66767349108428	34.0	30.0	36.0	22.5	37.5
78-79	32.73604715610128	35.0	32.0	37.0	25.5	37.0
80-81	32.77491283350225	35.0	32.0	36.5	25.0	37.0
82-83	32.56577241285085	35.0	32.0	36.0	24.5	37.5
84-85	32.35047950246383	35.0	32.0	36.0	24.5	37.0
86-87	32.1007103942813	35.0	32.0	35.5	24.5	37.0
88-89	31.93202640959421	35.0	32.0	36.0	23.0	36.5
90-91	31.770739702398394	34.5	31.0	35.5	23.0	36.5
92-93	31.597805066731137	34.5	31.0	35.0	23.0	36.5
94-95	31.472179437299097	34.5	31.0	35.0	21.0	36.0
96-97	31.258185551896155	34.0	31.0	35.0	21.0	36.5
98-99	31.096302568749856	34.0	30.5	35.0	20.0	36.5
100	29.853385763988403	34.0	29.0	35.0	18.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.12921938900986163
1101	2	-0.14016685670316775
1101	3	-0.1535758870996844
1101	4	-0.1439952261585944
1101	5	-0.1380525388089282
1101	6	-0.20854536008351943
1101	7	-0.19037446219136456
1101	8	-0.10168993648410662
1101	9	-0.199556748883019
1101	10-11	-0.1257947365762604
1101	12-13	-0.17717489604486758
1101	14-15	-0.30691579506174094
1101	16-17	-0.16524816170237244
1101	18-19	-0.23397801095548942
1101	20-21	-0.2538182567452125
1101	22-23	-0.19972372292200902
1101	24-25	-0.3524978344679468
1101	26-27	-0.32454822117200877
1101	28-29	-0.37654518949662474
1101	30-31	-0.3791608647770204
1101	32-33	-0.302017201013399
1101	34-35	-0.2632719371955332
1101	36-37	-0.5013720091513107
1101	38-39	-0.3814880455564804
1101	40-41	-0.3649835203128049
1101	42-43	-0.3426425895246936
1101	44-45	-0.4985494634862562
1101	46-47	-0.4349883009132185
1101	48-49	-0.6596695366358034
1101	50-51	-0.499115047297515
1101	52-53	-0.6306730894965682
1101	54-55	-0.5772312256717029
1101	56-57	-0.631842617490598
1101	58-59	-0.73753455410084
1101	60-61	-0.6449455975762461
1101	62-63	-0.6031018582267222
1101	64-65	-0.5959713606095889
1101	66-67	-0.6037189278425998
1101	68-69	-0.6429292455376299
1101	70-71	-0.6767495756911117
1101	72-73	-0.7677818394546279
1101	74-75	-0.8155281594628363
1101	76-77	-0.6856404672003897
1101	78-79	-0.7382764958357271
1101	80-81	-0.4966269269497303
1101	82-83	-0.6148366949569777
1101	84-85	-0.6763468765481271
1101	86-87	-0.6729985621308288
1101	88-89	-0.7248933413731713
1101	90-91	-0.7003145349642068
1101	92-93	-0.8845113801982194
1101	94-95	-0.7537253569185189
1101	96-97	-0.7778705067390064
1101	98-99	-0.7807033252812197
1101	100	-0.8579672626959223
1102	1	-0.0522573850346717
1102	2	-0.09482884506928713
1102	3	-0.0823155619128002
1102	4	-0.09482286077624735
1102	5	-0.10882388285205025
1102	6	-0.12569688464941464
1102	7	-0.18537214041798222
1102	8	-0.116394137131131
1102	9	-0.18708991061706115
1102	10-11	-0.15046685576927388
1102	12-13	-0.17422020964849594
1102	14-15	-0.25856305636354193
1102	16-17	-0.17318639303838523
1102	18-19	-0.19825699636010796
1102	20-21	-0.24313792968403192
1102	22-23	-0.18272961489280704
1102	24-25	-0.23202821173126864
1102	26-27	-0.21347179896904578
1102	28-29	-0.2328432182643212
1102	30-31	-0.29025025938025806
1102	32-33	-0.3213182229251572
1102	34-35	-0.2893803429191806
1102	36-37	-0.3566905727314378
1102	38-39	-0.43895647561581086
1102	40-41	-0.39884947036860297
1102	42-43	-0.35689333999537354
1102	44-45	-0.47800058254787814
1102	46-47	-0.36130077334122035
1102	48-49	-0.3201902210602441
1102	50-51	-0.3401521997396486
1102	52-53	-0.4694490553904558
1102	54-55	-0.4851941173749097
1102	56-57	-0.3114424483220617
1102	58-59	-0.5579912609372499
1102	60-61	-0.4802984355637747
1102	62-63	-0.41590234406646687
1102	64-65	-0.4136933920543271
1102	66-67	-0.5070870511144534
1102	68-69	-0.4803120909017977
1102	70-71	-0.3829122229225774
1102	72-73	-0.6371366795008697
1102	74-75	-0.9658513846697261
1102	76-77	-0.4075076099335533
1102	78-79	-0.4086318529699824
1102	80-81	-0.39810375034790724
1102	82-83	-0.33048819722420575
1102	84-85	-0.36374018571251554
1102	86-87	-0.4418244943657861
1102	88-89	-0.4453492270762318
1102	90-91	-0.3619516970791352
1102	92-93	-0.4710197185437295
1102	94-95	-0.532816002205788
1102	96-97	-0.6192852675225247
1102	98-99	-0.708912672249685
1102	100	-0.5371297081847217
1103	1	-0.13170717878960403
1103	2	-0.11612334618113351
1103	3	-0.15193300426090417
1103	4	0.002846822100302404
1103	5	-0.15882835969843256
1103	6	-0.13500226708848828
1103	7	-0.08992621331563555
1103	8	-0.13129594241304687
1103	9	-0.14723911839768533
1103	10-11	-0.12505732283752025
1103	12-13	-0.12342914687873474
1103	14-15	-0.20924592320607616
1103	16-17	-0.24462631686120773
1103	18-19	-0.19634351851305354
1103	20-21	-0.18728013125478782
1103	22-23	-0.1279348211988065
1103	24-25	-0.18589872455018508
1103	26-27	-0.3006320393483364
1103	28-29	-0.2436299005110385
1103	30-31	-0.18640593994373233
1103	32-33	-0.14236458926195894
1103	34-35	-0.05189061102426962
1103	36-37	-0.11350853248184478
1103	38-39	-0.15250498532947176
1103	40-41	-0.2546209681885969
1103	42-43	-0.3534603431345644
1103	44-45	-0.42840535221723286
1103	46-47	-0.300982948625915
1103	48-49	-0.3923242749894911
1103	50-51	-0.24965979188004184
1103	52-53	-0.29378050387538224
1103	54-55	-0.3228412284808897
1103	56-57	-0.24197770036777655
1103	58-59	-0.35630765291575983
1103	60-61	-0.34724519276173993
1103	62-63	-0.28138887218854336
1103	64-65	-0.3213973477987153
1103	66-67	-0.4744672049880734
1103	68-69	-0.31444274315752097
1103	70-71	-0.4886790505197709
1103	72-73	-0.5882162416353651
1103	74-75	-0.465549480306116
1103	76-77	-0.302329412839498
1103	78-79	-0.33738795080326156
1103	80-81	-0.28932037841347835
1103	82-83	-0.34313384706126726
1103	84-85	-0.2865409102975818
1103	86-87	-0.3132275745090709
1103	88-89	-0.317854625744328
1103	90-91	-0.26459280293535414
1103	92-93	-0.4240637113857346
1103	94-95	-0.2202299268983019
1103	96-97	-0.8790091457076414
1103	98-99	-1.2216631284538302
1103	100	-0.49537331911925975
1104	1	-0.06161987534834168
1104	2	-0.12357665873152257
1104	3	-0.08337695203515949
1104	4	-0.12943792938745702
1104	5	-0.21937486343305324
1104	6	-0.13714502256860328
1104	7	-0.1966990479052697
1104	8	-0.08702140014574411
1104	9	-0.12621100451109157
1104	10-11	-0.10846147946963924
1104	12-13	-0.12652750596257079
1104	14-15	-0.30826628575602655
1104	16-17	-0.09000039844195129
1104	18-19	-0.10479285855652165
1104	20-21	-0.27521181477188605
1104	22-23	-0.1799121526155787
1104	24-25	-0.20028537324677131
1104	26-27	-0.1728529747843055
1104	28-29	-0.23344862553636858
1104	30-31	-0.3037313068737575
1104	32-33	-0.22715711256017102
1104	34-35	-0.1836691940323405
1104	36-37	-0.22290904807048406
1104	38-39	-0.2726339469493766
1104	40-41	-0.30388872332992634
1104	42-43	-0.27402273074374506
1104	44-45	-0.37967367051677314
1104	46-47	-0.2735033109194873
1104	48-49	-0.3092065577082508
1104	50-51	-0.16938299097324716
1104	52-53	-0.1994378962316148
1104	54-55	-0.3969426274324164
1104	56-57	-0.38123730333977335
1104	58-59	-0.3097858251509038
1104	60-61	-0.2755943707800199
1104	62-63	-0.2609121046991163
1104	64-65	-0.403192434389787
1104	66-67	-0.49873267284854705
1104	68-69	-0.34614017073019454
1104	70-71	-0.3994731006741077
1104	72-73	-0.24485179908472787
1104	74-75	-0.37756633754777624
1104	76-77	-0.363428746315968
1104	78-79	-0.3086638235391703
1104	80-81	-0.19237693647807674
1104	82-83	-0.17043330050918115
1104	84-85	-0.2615657810433234
1104	86-87	-0.31665461347254364
1104	88-89	-0.2632906159054649
1104	90-91	-0.3086543112690734
1104	92-93	-0.417820881342827
1104	94-95	-0.41773565901826615
1104	96-97	-0.11477694798636762
1104	98-99	-1.1064694377010902
1104	100	-0.38699462657075756
1105	1	-0.1158013000379583
1105	2	-1.0985778000293216
1105	3	-0.15850314250855035
1105	4	-0.10076765554262579
1105	5	-0.1363758385878313
1105	6	-0.10955407253806015
1105	7	-0.170367320870632
1105	8	-0.14904321106835283
1105	9	-0.17980934273872862
1105	10-11	-0.07885556416501771
1105	12-13	-0.06454708824507804
1105	14-15	-0.11370102649040348
1105	16-17	-0.5769877681865481
1105	18-19	-0.08384378951875249
1105	20-21	-0.18940704159185628
1105	22-23	-0.13407985143954448
1105	24-25	-0.2160622873564293
1105	26-27	-0.16533683829457146
1105	28-29	-0.26200553581211494
1105	30-31	-0.19977658437591117
1105	32-33	-0.19325243360982824
1105	34-35	-0.16210061678861365
1105	36-37	-0.236837291390259
1105	38-39	-0.20277052029263132
1105	40-41	-0.19829325828608546
1105	42-43	-0.24965169274435084
1105	44-45	-0.3922847350078129
1105	46-47	-0.2789706263065952
1105	48-49	-0.2986384162781235
1105	50-51	-0.24921172039500306
1105	52-53	-0.2925355534403593
1105	54-55	-0.32885525081329803
1105	56-57	-0.2171775978377255
1105	58-59	-0.33967461481251604
1105	60-61	-0.27357881748365287
1105	62-63	-0.15001937853122627
1105	64-65	-0.15727769248933754
1105	66-67	-0.17545060044703575
1105	68-69	-0.23774708275040268
1105	70-71	-0.30167517642128416
1105	72-73	-0.35390700906730643
1105	74-75	-0.34276101337304965
1105	76-77	-0.11275895329517738
1105	78-79	-0.1793026716199151
1105	80-81	-0.14980749077887623
1105	82-83	-0.06696834711850386
1105	84-85	-0.03090794554480425
1105	86-87	-0.14169519324683577
1105	88-89	-0.07254794696639877
1105	90-91	-0.14597151340487358
1105	92-93	-0.2472497912482261
1105	94-95	-0.20486137258776083
1105	96-97	-0.22400005615028462
1105	98-99	-0.162948203093773
1105	100	-0.03946686292036361
1106	1	-0.11693172214320668
1106	2	-0.14859456031798857
1106	3	-0.11260918543477061
1106	4	-0.08240104024288541
1106	5	-0.11540835851793929
1106	6	-0.07213655552374121
1106	7	-0.11316113535770711
1106	8	-0.054389567127572036
1106	9	-0.12161220861003841
1106	10-11	-0.14999743269145682
1106	12-13	-0.07066264353249352
1106	14-15	-0.13479178407477832
1106	16-17	-0.03413948276131862
1106	18-19	-0.10500028015452756
1106	20-21	-0.12457159407833984
1106	22-23	-0.06508553295650898
1106	24-25	-0.0033475185462421564
1106	26-27	-0.04876650244544578
1106	28-29	-0.05246588564931187
1106	30-31	-0.10500093996076032
1106	32-33	0.018630438479625866
1106	34-35	-0.012471586588070238
1106	36-37	-0.10094183534749845
1106	38-39	-0.12230159081671843
1106	40-41	-0.10920060633349493
1106	42-43	-0.09989085992367563
1106	44-45	-0.1053804500619222
1106	46-47	-0.16110609658593233
1106	48-49	-0.17155527047411567
1106	50-51	-0.028916096307575856
1106	52-53	-0.09960823648921746
1106	54-55	-0.0842785295565065
1106	56-57	-0.11555436940300012
1106	58-59	-0.28532298499178665
1106	60-61	-0.16551976418040937
1106	62-63	-0.10965758512787716
1106	64-65	-0.13943227350144838
1106	66-67	-0.040393383182333764
1106	68-69	-0.01884502006140565
1106	70-71	-0.06873095318473332
1106	72-73	-0.055805524207890755
1106	74-75	-0.1960720228946613
1106	76-77	-0.18292419676391347
1106	78-79	-0.13118829555823908
1106	80-81	-0.0010757279471818038
1106	82-83	0.021496527444405444
1106	84-85	0.07534522634672669
1106	86-87	0.048880773589878856
1106	88-89	0.0013767904327401936
1106	90-91	0.012005122490421627
1106	92-93	-0.09813468340805542
1106	94-95	-0.10997863800446694
1106	96-97	-0.15024446616603626
1106	98-99	-0.15975663824056952
1106	100	0.02283322852951386
1107	1	-0.004767628942708768
1107	2	-0.026941224073794956
1107	3	-0.03249178895779181
1107	4	-0.045716570736367146
1107	5	0.013662437161634955
1107	6	-0.052857128809094434
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2316	76-77	-0.05209379965567962
2316	78-79	-0.039858173846901934
2316	80-81	0.009996711499049127
2316	82-83	-0.0011873628771965627
2316	84-85	0.1527106038920536
2316	86-87	-0.06218257541077321
2316	88-89	0.01796432941096171
2316	90-91	-0.07309955348718944
2316	92-93	-0.06943983658374009
2316	94-95	0.01863704479198347
2316	96-97	0.07717027149242384
2316	98-99	0.22773027956838376
2316	100	0.02822966587990905
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	6.0
12	27.0
13	179.0
14	663.0
15	1825.0
16	4133.0
17	8033.0
18	12939.0
19	19814.0
20	27769.0
21	38020.0
22	49488.0
23	58117.0
24	63893.0
25	70151.0
26	81939.0
27	98269.0
28	119875.0
29	146642.0
30	182798.0
31	228683.0
32	294125.0
33	397192.0
34	605179.0
35	1116997.0
36	1402249.0
37	1200201.0
38	1083451.0
39	182140.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42743866932934	19.230096268329333	13.438541471615784	23.903923590725537
2	16.32142840453393	20.945117697441372	39.09545772859059	23.637996169434114
3	19.121282571034236	25.77341362478211	30.0105034280243	25.094800376159355
4	13.43845081930904	16.9369321769129	37.15468964563943	32.46992735813863
5	13.678715038411498	37.58153195278675	34.407794991728224	14.331958017073527
6	31.407002087457048	36.601721334825044	17.79807639065401	14.193200187063903
7	27.57922739224569	32.35525295966524	22.135863674941636	17.929655973147433
8	26.07499056013428	35.14147790270867	20.317286228736954	18.466245308420092
9	26.831554089600978	15.580750037592475	20.02782977273331	37.559866100073236
10-11	25.089139536539122	25.810361394133214	28.454203322668008	20.646295746659657
12-13	25.59936708484974	23.550691885694476	28.36244012299923	22.487500906456553
14-15	23.00829833162022	25.060036772648342	25.94169030619455	25.989974589536892
16-17	22.707755194567845	27.87208531168341	26.611453990656024	22.80870550309272
18-19	22.61833927442199	27.00865402236486	28.35579445774582	22.01721224546733
20-21	23.306914254027706	26.30064887343766	28.258250080455394	22.134186792079237
22-23	23.120936175044545	26.05055606224631	28.155140636204777	22.67336712650437
24-25	22.896419991432726	26.18344544327764	28.157827549912767	22.76230701537686
26-27	22.746672249556234	26.302048202593774	28.250155083972185	22.70112446387781
28-29	22.726719880249757	26.531482842645865	28.226519093368	22.515278183736378
30-31	23.215532883540334	26.252516308131955	28.146282535867485	22.385668272460226
32-33	22.996263427525474	26.336887986557546	28.092860214293314	22.573988371623667
34-35	23.1372325589215	26.469755080626612	28.044733318140068	22.348279042311823
36-37	23.07998631810434	26.39198844550969	27.992546075491532	22.535479160894436
38-39	22.99048861711582	26.59804795459802	27.92157180357219	22.48989162471397
40-41	23.023745825578583	26.357165220859308	28.061278508270256	22.557810445291853
42-43	22.892159210796155	26.48447051486622	27.75596841679561	22.867401857542013
44-45	22.75674988683334	26.564366895490714	27.861261842470004	22.817621375205942
46-47	22.825387011736076	26.457297880785923	27.636850548604553	23.080464558873445
48-49	22.87131986325523	26.57290346429199	27.56168410784114	22.994092564611638
50-51	22.833511989966226	26.7576550019209	27.525533798129704	22.88329920998317
52-53	22.90712136559124	26.8365640745702	27.254954994504494	23.00135956533406
54-55	22.70874584376284	26.646216823487347	27.50893290779158	23.136104424958237
56-57	22.831589697444215	26.75557768679774	27.215443639785214	23.197388975972835
58-59	22.759645787682757	26.671819225895245	27.423267841125494	23.1452671452965
60-61	22.980864607208876	26.73103439625949	27.456457281074865	22.831643715456767
62-63	22.832604098729007	26.740876383793378	27.45315437108452	22.97336514639309
64-65	22.69825026370701	26.519541805275047	27.43886537302625	23.343342557991694
66-67	22.64930225830886	26.56790904587108	27.29041497307623	23.492373722743828
68-69	22.457068367103833	26.655797642675623	27.374975923646318	23.51215806657423
70-71	22.56937221866347	26.40873573665449	27.384209073383055	23.63768297129899
72-73	22.5040134557764	26.701524782951818	27.07167071642696	23.722791044844826
74-75	22.48000847577379	26.77016175357782	27.030606323391602	23.719223447256788
76-77	22.43715599084644	26.52338094698006	27.152855150880328	23.886607911293172
78-79	22.312928601150322	26.488364184910516	27.219512572633985	23.979194641305174
80-81	22.329097561688297	26.66976391818506	26.932265324156678	24.06887319596996
82-83	22.474573396861285	26.549741395936906	27.01463295982119	23.961052247380618
84-85	22.403453971310483	26.634304643236977	26.867032885364544	24.095208500087995
86-87	22.16871737925969	26.660987137333752	27.032620490136267	24.13767499327029
88-89	22.088071744906053	26.42269095167087	27.080275647998022	24.40896165542506
90-91	22.369667797475856	26.31335233900446	26.95534579031367	24.361634073206012
92-93	22.284112304904642	26.313050392013636	26.948421835136056	24.454415467945665
94-95	22.113035029475206	26.165839023298098	26.97340173299422	24.747724214232477
96-97	21.994273373655933	26.07569049852231	27.120497256739302	24.80953887108246
98-99	21.943437498832495	26.042422209448603	26.998595791216413	25.015544500502497
100	21.93807656348051	26.00032850700239	27.079171121592555	24.982423807924544
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1051.0
1	835.5
2	1090.0
3	1496.0
4	1143.5
5	998.0
6	1492.0
7	2774.5
8	4409.5
9	5411.0
10	5148.0
11	4680.5
12	5055.5
13	5562.5
14	6129.0
15	6766.0
16	7447.5
17	8090.5
18	8734.0
19	9583.0
20	10415.0
21	11717.0
22	13740.0
23	16464.0
24	20257.0
25	25344.5
26	32110.0
27	40229.5
28	48792.5
29	58828.5
30	71122.0
31	83129.0
32	96585.5
33	111508.5
34	125650.5
35	140767.0
36	161340.5
37	180955.5
38	193973.0
39	205448.5
40	213811.5
41	220491.0
42	229029.5
43	241833.0
44	254505.0
45	267166.5
46	278246.5
47	287138.0
48	301019.5
49	310469.0
50	309217.0
51	303865.0
52	295990.0
53	286657.0
54	275656.0
55	256965.0
56	232716.5
57	209361.5
58	186798.5
59	161551.5
60	135140.5
61	111522.5
62	91317.0
63	71756.5
64	53654.5
65	40614.0
66	30841.0
67	23750.0
68	18529.5
69	14110.5
70	11109.0
71	8592.5
72	6642.5
73	5307.0
74	4210.5
75	3227.0
76	2203.5
77	1383.0
78	924.5
79	614.0
80	398.0
81	255.5
82	165.0
83	105.0
84	66.5
85	51.5
86	40.0
87	27.0
88	22.0
89	16.0
90	6.5
91	3.5
92	3.5
93	2.5
94	0.5
95	0.5
96	0.5
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028673014490479598
2	0.06800667978500442
3	0.0020814286926546383
4	0.0
5	0.0012141667373818722
6	0.0011607967709035482
7	1.3342491619581015E-5
8	5.603846480224026E-4
9	1.3342491619581015E-5
10-11	2.001373742937152E-5
12-13	1.1341117876643861E-4
14-15	0.009273031675608804
16-17	0.0010073581172783666
18-19	1.801236368643437E-4
20-21	2.1347986591329623E-4
22-23	0.0019013050557902945
24-25	6.871383184084222E-4
26-27	0.007004808100280032
28-29	0.007838713826503845
30-31	0.009846758815250788
32-33	0.009132935513603203
34-35	0.004056117452352628
36-37	0.00368919893281415
38-39	0.0026751695697259936
40-41	0.0033422941507050435
42-43	0.0014543315865343305
44-45	0.00499676311153309
46-47	0.016658100787046895
48-49	0.017738842608232958
50-51	0.012822134446417355
52-53	0.0072716579326716525
54-55	0.0012275092290014531
56-57	0.0022415385920896104
58-59	0.002595114620008507
60-61	0.0034423628378519016
62-63	0.0031488280222211193
64-65	0.0035424315249987593
66-67	0.0023082510501875155
68-69	0.006210929848914961
70-71	3.8693225696784944E-4
72-73	0.005010105603152671
74-75	0.007351712882389138
76-77	0.006697930793029668
78-79	0.004543118396467335
80-81	4.6698720668533544E-4
82-83	0.001247522966430825
84-85	7.872070055552798E-4
86-87	0.0013275779161483108
88-89	0.001014029363088157
90-91	9.539881508000425E-4
92-93	3.1354855306015385E-4
94-95	0.0010407143463273191
96-97	0.0028352794691609653
98-99	0.003202197988699443
100	0.004523104659037963
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7494852.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.91054263975288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.13073042498635	41.44689364342225
2	14.686936712302762	16.423092026011112
3	4.963746783496432	8.325773285748403
4	2.1658607698928787	4.843778037074552
5	1.1481767572654824	3.209759277253247
6	0.7122179652556133	2.38922957491332
7	0.4329998105916711	1.6946478061163381
8	0.31848228172099813	1.4245213753734123
9	0.22434675100338122	1.1289013729258113
>10	1.1186579560686212	10.930027755424621
>50	0.06196941653339778	2.3866654039550625
>100	0.03281411035925645	3.371371669143033
>500	0.0022867912017917255	0.8805677699133702
>1k	6.725820186580661E-4	0.6628344084039789
>5k	0.0	0.0
>10k+	1.0088730279332775E-4	0.8819365943216558
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19523	0.2604854638890801	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17122	0.2284501415104661	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10261	0.1369073065085208	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3342491619581015E-5
2	0.0	0.0	0.0	0.0	1.3342491619581015E-5
3	0.0	0.0	0.0	0.0	1.3342491619581015E-5
4	0.0	0.0	0.0	0.0	4.002747485874304E-5
5	0.0	0.0	0.0	0.0	4.002747485874304E-5
6	5.336996647832406E-5	0.0	0.0	0.0	1.2008242457622912E-4
7	5.336996647832406E-5	0.0	0.0	0.0	1.2008242457622912E-4
8	5.336996647832406E-5	0.0	0.0	0.0	1.2008242457622912E-4
9	5.336996647832406E-5	0.0	0.0	0.0	1.2008242457622912E-4
10-11	5.336996647832406E-5	0.0	0.0	0.0	1.2675367038601963E-4
12-13	5.336996647832406E-5	0.0	0.0	0.0	1.5343865362518164E-4
14-15	5.336996647832406E-5	6.671245809790507E-6	0.0	0.0	1.9346612848392472E-4
16-17	6.671245809790506E-5	1.3342491619581015E-5	0.0	6.671245809790507E-6	2.935348156307823E-4
18-19	6.671245809790506E-5	6.004121228811456E-5	0.0	4.669872066853355E-5	3.202197988699443E-4
20-21	6.671245809790506E-5	6.671245809790506E-5	0.0	1.0673993295664812E-4	3.2689104467973484E-4
22-23	7.338370390769557E-5	6.671245809790506E-5	0.0	2.6684983239162025E-4	3.6024727372868735E-4
24-25	8.005494971748608E-5	6.671245809790506E-5	0.0	7.271657932671653E-4	4.0027474858743043E-4
26-27	8.005494971748608E-5	6.671245809790506E-5	0.0	0.0015077015530126545	4.136172402070114E-4
28-29	9.33974413370671E-5	6.671245809790506E-5	0.0	0.004423035971891106	4.136172402070114E-4
30-31	9.33974413370671E-5	6.671245809790506E-5	0.0	0.014963604351360108	4.3363097763638293E-4
32-33	9.33974413370671E-5	6.671245809790506E-5	0.0	0.042589233249702596	4.6031596087554497E-4
34-35	9.33974413370671E-5	8.005494971748608E-5	0.0	0.08518513774521498	4.803296983049165E-4
36-37	9.33974413370671E-5	8.005494971748608E-5	0.0	0.1522911993458977	4.803296983049165E-4
38-39	9.33974413370671E-5	8.672619552727659E-5	0.0	0.2490376060794796	5.00343435734288E-4
40-41	9.33974413370671E-5	1.0006868714685761E-4	0.0	0.3576321453712495	5.203571731636596E-4
42-43	9.33974413370671E-5	1.0673993295664812E-4	0.0	0.46393177610445147	5.2702841897345E-4
44-45	9.33974413370671E-5	1.0673993295664812E-4	0.0	0.6017330295514842	5.40370910593031E-4
46-47	9.33974413370671E-5	1.0673993295664812E-4	0.0	0.7642178924947418	5.670558938321931E-4
48-49	9.33974413370671E-5	1.1341117876643861E-4	0.0	0.9354420874488248	6.404395977398886E-4
50-51	9.33974413370671E-5	1.2008242457622912E-4	0.0	1.1145250099668411	6.404395977398886E-4
52-53	9.33974413370671E-5	1.2008242457622912E-4	0.0	1.2998655610544412	6.737958267888412E-4
54-55	9.33974413370671E-5	1.5343865362518164E-4	0.0	1.5110238334259303	7.271657932671653E-4
56-57	9.33974413370671E-5	1.7345239105455318E-4	0.0	1.7524028493157704	7.671932681259083E-4
58-59	9.33974413370671E-5	1.7345239105455318E-4	0.0	2.0180852136906773	7.938782513650703E-4
60-61	1.0673993295664812E-4	1.7345239105455318E-4	0.0	2.298450990092933	8.339057262238133E-4
62-63	1.2008242457622912E-4	1.7345239105455318E-4	0.0	2.58546799856755	8.806044468923469E-4
64-65	1.2008242457622912E-4	1.7345239105455318E-4	0.0	2.882018217304358	8.806044468923469E-4
66-67	1.2008242457622912E-4	1.867948826741342E-4	0.0	3.216888071972602	8.806044468923469E-4
68-69	1.2008242457622912E-4	1.867948826741342E-4	0.0	3.5946006672313207	8.806044468923469E-4
70-71	1.2008242457622912E-4	1.9346612848392472E-4	0.0	4.0032678430474675	8.93946938511928E-4
72-73	1.2008242457622912E-4	2.0013737429371521E-4	0.0	4.40156123162939	9.33974413370671E-4
74-75	1.2008242457622912E-4	2.0013737429371521E-4	0.0	4.803984121367574	9.406456591804615E-4
76-77	1.2008242457622912E-4	2.4016484915245824E-4	0.0	5.238288894830745	9.673306424196234E-4
78-79	1.2008242457622912E-4	2.4016484915245824E-4	0.0	5.726724156794557	9.87344379848995E-4
80-81	1.2008242457622912E-4	2.5350734077203926E-4	0.0	6.2336988108637765	0.0010006868714685759
82-83	1.2008242457622912E-4	2.6684983239162025E-4	0.0	6.754322833859828	0.001094084312805643
84-85	1.2008242457622912E-4	2.6684983239162025E-4	0.0	7.27142443906831	0.0011007555586154336
86-87	1.2008242457622912E-4	2.6684983239162025E-4	0.0	7.798759735349011	0.0011541255250937577
88	1.2008242457622912E-4	2.6684983239162025E-4	0.0	8.219535222309927	0.0012008242457622912
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15345	0.0	39.975574	1
GTATCAA	23840	0.0	32.95295	1
GTACATG	25210	0.0	27.48615	1
TCAACGC	28685	0.0	26.916967	4
CAACGCA	28990	0.0	26.585146	5
ATCAACG	29040	0.0	26.55591	3
TATCAAC	29845	0.0	26.09462	2
TACATGG	26400	0.0	25.954811	2
AACGCAG	29960	0.0	25.786983	6
ACATGGG	26875	0.0	24.813221	3
CATGGGG	18950	0.0	22.815083	4
ACGCAGA	34075	0.0	22.672567	7
TACCTGG	6185	0.0	22.43079	2
CGCAGAG	35380	0.0	21.879778	8
ATGGGGG	11655	0.0	21.20884	5
GAGTACT	20595	0.0	20.345558	12-13
GCAGAGT	39315	0.0	19.41476	9
AGAGTAC	31990	0.0	19.0855	10-11
GTACCTG	7610	0.0	18.544737	1
GTACTTT	22385	0.0	18.47576	14-15
>>END_MODULE