FastQCFastQC Report
Mon 6 Jun 2016
ERR522855_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522855_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11561496
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323010.2793842596148457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268890.23257370845433845No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT196140.16964932565820204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156950.13575232824541045No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA141100.12204302972556494No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG196800.051.3009872
GTACCTG225750.045.238411
ACCTGGG228700.043.7755933
CCTGGGG205250.032.3239864
GTACCCG20550.024.4932941
CTGGGGG135150.022.5779845
CATGGGG292800.022.1609864
GTACATG573850.021.7641351
TACATGG583100.021.15312
ACATGGG588250.020.4005223
TACCCGG24800.019.9093192
TGGGGGG166950.019.7884836
GAGTACT297350.019.34326412-13
ATGGGGG173650.018.9260045
GTATCAA706550.018.395571
TATAACG10950.017.6071262
GTACTTT333600.017.21356614-15
AGTACTT315200.016.94335712-13
GTATAGG29200.016.9153791
TATCACG12450.016.6188932
Produced by FastQC (version 0.11.5)