##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522855_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11561496
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11920083698511	33.0	31.0	34.0	27.0	34.0
2	31.455335018928345	34.0	31.0	34.0	27.0	35.0
3	31.411667400135762	33.0	31.0	34.0	26.0	36.0
4	33.69546432399406	35.0	33.0	37.0	30.0	37.0
5	33.776904909191686	35.0	34.0	37.0	30.0	37.0
6	34.00241517187741	35.0	35.0	37.0	30.0	37.0
7	33.87946326323168	35.0	34.0	37.0	30.0	37.0
8	33.963966254886046	35.0	34.0	37.0	30.0	37.0
9	35.11517765521002	37.0	34.0	39.0	29.0	39.0
10-11	35.07161525636475	37.0	34.5	39.0	30.0	39.0
12-13	35.098251601695836	37.0	35.0	39.0	29.5	39.0
14-15	35.837421904570135	38.0	35.0	40.0	29.5	41.0
16-17	35.99647221259256	38.0	36.0	40.0	28.5	41.0
18-19	35.84165319090194	38.0	34.5	40.0	28.5	41.0
20-21	35.93508971503341	38.0	35.0	40.0	28.5	41.0
22-23	35.78398262646979	38.0	35.0	40.0	28.5	41.0
24-25	35.75509233407165	37.5	35.0	40.0	27.5	41.0
26-27	35.641765909878785	37.0	35.5	40.0	27.0	41.0
28-29	35.406536792470455	37.0	34.5	40.0	27.0	41.0
30-31	35.22797430367143	37.0	34.5	40.0	27.0	41.0
32-33	35.19906961002279	37.5	34.0	40.0	26.0	41.0
34-35	35.05010056657028	37.5	34.0	40.0	25.0	41.0
36-37	34.875169787716054	37.5	34.0	40.0	25.0	41.0
38-39	34.712444522750346	36.5	33.5	40.0	25.0	41.0
40-41	34.66362350512425	37.0	33.0	40.0	24.5	41.0
42-43	34.462903805874255	36.0	34.0	40.0	24.0	41.0
44-45	34.37498447432755	37.0	33.0	40.0	24.0	41.0
46-47	34.169294830011616	36.5	33.0	40.0	23.0	41.0
48-49	34.0339338438555	37.0	33.0	40.0	22.0	41.0
50-51	33.02343446730423	35.5	31.5	38.5	21.0	40.0
52-53	33.25680482871766	36.5	32.0	38.5	22.0	39.5
54-55	34.110880676687515	37.0	33.0	39.5	23.0	40.5
56-57	34.25033935919711	36.5	33.0	40.0	23.0	41.0
58-59	34.177701787035176	37.0	33.0	40.0	22.0	41.0
60-61	33.92135810106235	36.5	33.0	39.0	22.0	41.0
62-63	33.746286336993066	36.0	32.5	39.0	22.0	41.0
64-65	33.35206641943223	36.0	32.5	39.0	21.5	41.0
66-67	33.15410051605778	36.0	32.0	38.0	21.0	40.5
68-69	32.80857797295437	35.0	31.5	37.5	20.0	40.0
70-71	32.5935786770155	35.0	31.0	37.0	20.0	39.5
72-73	32.267370070447626	35.0	31.0	37.0	20.0	39.0
74-75	31.894262040137363	35.0	31.0	36.5	19.0	39.0
76-77	31.767399694641593	35.0	31.0	37.0	18.5	38.5
78-79	31.492184575421724	35.0	30.5	36.0	18.0	38.0
80-81	31.221878595987924	35.0	29.5	36.0	18.0	37.5
82-83	30.91170173825256	34.0	29.5	35.5	17.5	37.0
84-85	30.674902192588227	34.0	29.0	35.0	17.5	37.0
86-87	30.4316126563552	34.0	29.0	35.0	17.0	37.0
88-89	30.26980453048637	34.0	29.0	35.0	17.5	36.5
90-91	30.051983151661343	34.0	29.0	35.0	17.0	36.0
92-93	29.793843331347432	34.0	28.0	35.0	16.5	36.0
94-95	29.6881652253307	34.0	27.5	35.0	16.0	36.0
96-97	29.497771568662046	34.0	27.0	35.0	15.0	36.0
98-99	29.405423830964438	34.0	27.0	35.0	13.5	36.0
100	28.145709084706684	32.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14220469545478664
1101	2	0.023926669540134782
1101	3	0.0737318999874077
1101	4	0.05505497208848453
1101	5	0.11558638345879046
1101	6	0.18683916562048353
1101	7	0.2157946386706584
1101	8	0.18237590001371018
1101	9	0.2204497292333869
1101	10-11	0.16682767028087397
1101	12-13	0.11279256110943692
1101	14-15	0.01786562842949735
1101	16-17	0.06587575201937312
1101	18-19	0.10736548897769183
1101	20-21	0.0829940993093885
1101	22-23	0.06970568201617056
1101	24-25	0.06713747327287223
1101	26-27	0.09863199290730762
1101	28-29	0.02012260543624933
1101	30-31	-0.07371432842889902
1101	32-33	-0.07371579633342407
1101	34-35	-0.07575413250653895
1101	36-37	-0.09073013328403334
1101	38-39	-0.16288785947572393
1101	40-41	-0.15372828142574235
1101	42-43	-0.13693419344496505
1101	44-45	-0.020536709311549828
1101	46-47	-0.10384764440012617
1101	48-49	-0.18696352014396211
1101	50-51	-0.20008830349726736
1101	52-53	-0.07192204476967134
1101	54-55	0.059933850877897044
1101	56-57	-0.05867874417640451
1101	58-59	-0.06841790572407547
1101	60-61	-0.07184766411337762
1101	62-63	-0.15943395396354987
1101	64-65	-0.11735514382490919
1101	66-67	-0.18309196785711634
1101	68-69	-0.06130513057715348
1101	70-71	-0.16790636966480577
1101	72-73	-0.18520047893075997
1101	74-75	-0.24159836369649668
1101	76-77	-0.1337940747559614
1101	78-79	-0.3230107301099032
1101	80-81	-0.2582168647911267
1101	82-83	-0.3758153607385317
1101	84-85	-0.3453340363615496
1101	86-87	-0.29778637363751415
1101	88-89	-0.42204995860336325
1101	90-91	-0.43370838419718893
1101	92-93	-0.41816001402166947
1101	94-95	-0.4336423178201656
1101	96-97	-0.46089592040515015
1101	98-99	-0.45995231191905006
1101	100	-0.43554710883280734
1102	1	0.024607428188847535
1102	2	-0.00422073303217374
1102	3	0.013609329848605967
1102	4	0.043987703923008326
1102	5	0.006567087311672992
1102	6	0.046592331722642655
1102	7	0.04235977621171827
1102	8	0.12544732147097903
1102	9	0.059597680721260815
1102	10-11	0.06455554434445787
1102	12-13	-0.03493820638279743
1102	14-15	-0.0434176165031559
1102	16-17	0.08816382492708641
1102	18-19	0.06483971511894993
1102	20-21	-0.07293113140778473
1102	22-23	-0.011086352927698329
1102	24-25	-0.057136444917560425
1102	26-27	-0.0963321327567428
1102	28-29	-0.048935057253366665
1102	30-31	-0.12213025500473407
1102	32-33	-0.0317851927121211
1102	34-35	-0.13950534348681032
1102	36-37	-0.5304492282992754
1102	38-39	-0.2605587426395246
1102	40-41	-0.9182501989227774
1102	42-43	-0.9877904441799785
1102	44-45	-0.44490377018536975
1102	46-47	-0.3433623652971036
1102	48-49	-0.44077090254071294
1102	50-51	-0.48332526315187607
1102	52-53	-0.28214260086994614
1102	54-55	-0.1358026916407553
1102	56-57	-0.009802698145676914
1102	58-59	-0.10834079134947672
1102	60-61	-0.1633352661252232
1102	62-63	-0.34161561106615324
1102	64-65	-0.11497576491801453
1102	66-67	-0.11785927318064893
1102	68-69	-0.167746036186891
1102	70-71	-0.3317847838061354
1102	72-73	-0.23604255347280656
1102	74-75	-0.3605411476236675
1102	76-77	-0.4056720041515476
1102	78-79	-0.25229645895461417
1102	80-81	-0.3896133594858959
1102	82-83	-0.4395771086214886
1102	84-85	-0.5512914870142893
1102	86-87	-0.4897306037003304
1102	88-89	-0.4536877443511429
1102	90-91	-0.4703972042396245
1102	92-93	-0.4923182448970884
1102	94-95	-0.6108745160485753
1102	96-97	-0.7886809274680111
1102	98-99	-0.656843653963918
1102	100	-0.652536865388079
1103	1	0.007339302655697821
1103	2	-0.03194455825630982
1103	3	-0.016901737013878204
1103	4	-0.016405208392839654
1103	5	0.08344542931465071
1103	6	0.0934165104088649
1103	7	0.11993044964406607
1103	8	0.007948751216950711
1103	9	0.048852535852574874
1103	10-11	-0.0031137487803505337
1103	12-13	0.07070307236177342
1103	14-15	-0.03801301341476915
1103	16-17	0.03220417154910393
1103	18-19	-0.00617025512271141
1103	20-21	-0.1316753429996922
1103	22-23	-0.08951411687824162
1103	24-25	-0.08332329420992579
1103	26-27	-0.12233904991936839
1103	28-29	-0.29124828690996196
1103	30-31	-0.24522250869488005
1103	32-33	-0.3722485987858377
1103	34-35	-0.39627352255367754
1103	36-37	-0.5018771634974826
1103	38-39	-0.38688511335452347
1103	40-41	-0.22503863011485237
1103	42-43	-0.30750337706330555
1103	44-45	-0.28319153738646463
1103	46-47	-0.22296400019939
1103	48-49	-0.3001985843158437
1103	50-51	-0.3875933872422408
1103	52-53	-0.21329328275385961
1103	54-55	-0.10312874901739377
1103	56-57	0.0496659112029505
1103	58-59	0.036945043135986566
1103	60-61	-0.05739712612155756
1103	62-63	-0.03107602602138826
1103	64-65	-0.17512919448584796
1103	66-67	-0.1071283262828544
1103	68-69	-0.17681483352901495
1103	70-71	-0.15296559345133431
1103	72-73	-0.17709776284151246
1103	74-75	-0.1326179142369135
1103	76-77	-0.1261102908358609
1103	78-79	-0.21161245478182167
1103	80-81	-0.12066963556534915
1103	82-83	-0.1871073791155844
1103	84-85	-0.19132549920088593
1103	86-87	-0.8295063872194355
1103	88-89	-0.32067271034093636
1103	90-91	-0.6242473562387758
1103	92-93	-0.37185841075063664
1103	94-95	-0.7365899974637173
1103	96-97	-0.42734915589562306
1103	98-99	-0.3849856795883433
1103	100	-0.4201945472521551
1104	1	0.08140676803893143
1104	2	-0.02680584014953169
1104	3	-0.018461681585762335
1104	4	-0.0019686097183750917
1104	5	-0.049694010026684055
1104	6	0.12863434334234114
1104	7	0.05601223986616333
1104	8	0.06500590185603983
1104	9	0.04999465193232311
1104	10-11	-0.05238271718740606
1104	12-13	0.02825573131529069
1104	14-15	-0.019351877514388605
1104	16-17	0.021553656055509407
1104	18-19	0.004707488921070535
1104	20-21	-0.05984080942289438
1104	22-23	0.026917531444887288
1104	24-25	0.023162144836845755
1104	26-27	0.05777207023718489
1104	28-29	0.017224452855430172
1104	30-31	0.025330997284470413
1104	32-33	0.017319091030586264
1104	34-35	-0.12027292244105325
1104	36-37	-0.04159482993515695
1104	38-39	-0.056773591852739
1104	40-41	-0.04525445405778328
1104	42-43	-0.25377178157251734
1104	44-45	-0.1664802630977178
1104	46-47	-0.08247966989614497
1104	48-49	-0.0651298370796809
1104	50-51	-0.2556790893466072
1104	52-53	-0.13782664513812648
1104	54-55	-0.0227179910725539
1104	56-57	-0.04935960930941263
1104	58-59	0.014335251908427438
1104	60-61	-0.10245789611377631
1104	62-63	-0.007311734793233882
1104	64-65	-0.0678298249742113
1104	66-67	-0.039291156039688246
1104	68-69	-0.10395444905621076
1104	70-71	-0.027541289948885606
1104	72-73	0.03194398060715997
1104	74-75	-0.008237323054768098
1104	76-77	-0.06583713121338519
1104	78-79	-0.12962498009487788
1104	80-81	-0.10484791174136987
1104	82-83	-0.06518483615224469
1104	84-85	-0.10035298630486267
1104	86-87	-0.11338724862248029
1104	88-89	-0.11096796253464447
1104	90-91	-0.23090706812880768
1104	92-93	-0.1835855918187228
1104	94-95	-0.18014562056256622
1104	96-97	-0.22186990182867916
1104	98-99	-0.2830987510947729
1104	100	-0.16829233094103913
1105	1	0.1513926025246377
1105	2	0.13207811282209647
1105	3	0.16514312132976983
1105	4	0.09617136884612876
1105	5	0.13137210927514786
1105	6	0.21451786191066446
1105	7	0.10940801777538667
1105	8	0.07098533423934583
1105	9	0.07055385110452761
1105	10-11	0.20145057633869357
1105	12-13	0.23264947592630136
1105	14-15	0.12335799914510659
1105	16-17	0.08524220340547828
1105	18-19	0.1132885298002293
1105	20-21	0.09403897151582896
1105	22-23	0.11738991347541372
1105	24-25	0.12153514285780886
1105	26-27	0.10504729797511203
1105	28-29	0.008688492326605513
1105	30-31	-0.0180954996213174
1105	32-33	0.007139204948892086
1105	34-35	0.010345890047126716
1105	36-37	-0.006415876847590596
1105	38-39	-0.003121456044759441
1105	40-41	0.05311016914700417
1105	42-43	0.045630727126386716
1105	44-45	-0.05636193288557223
1105	46-47	-0.05222258889782694
1105	48-49	0.007645044149739988
1105	50-51	-0.06768658939245853
1105	52-53	0.07155214570862967
1105	54-55	0.177190183358384
1105	56-57	0.1310753082119689
1105	58-59	0.19728994855581306
1105	60-61	0.12938628282522302
1105	62-63	0.12969667029186382
1105	64-65	0.09243083983973577
1105	66-67	0.13570310505637906
1105	68-69	0.1332276521235869
1105	70-71	0.1554075517041582
1105	72-73	0.07390193777198562
1105	74-75	0.13081424598043512
1105	76-77	0.22069518750895867
1105	78-79	0.2357018956197301
1105	80-81	0.15864887134302919
1105	82-83	0.1495520432752393
1105	84-85	0.18053226414593837
1105	86-87	0.11813350400116462
1105	88-89	0.08049747312748678
1105	90-91	0.03136067865023762
1105	92-93	0.0618860631519631
1105	94-95	0.13831117516241065
1105	96-97	0.1340889674107082
1105	98-99	0.04536042110562377
1105	100	0.14066175073004672
1106	1	0.006807137979357947
1106	2	-0.027816424390827876
1106	3	0.046841826894080896
1106	4	0.10451442414427703
1106	5	0.01109535041882026
1106	6	0.0569121829397119
1106	7	0.06920073597229504
1106	8	-1.1141083571558426
1106	9	0.07298949305648961
1106	10-11	0.06041959415018994
1106	12-13	-0.01742936954809693
1106	14-15	-0.0641903451814656
1106	16-17	-0.044368061032351136
1106	18-19	0.060046689911729345
1106	20-21	-0.003450581279224707
1106	22-23	0.03673142611179259
1106	24-25	0.04524918076150186
1106	26-27	-0.024385452995538515
1106	28-29	-0.12196925533284997
1106	30-31	-0.005096618186030355
1106	32-33	-0.06693426510895506
1106	34-35	0.028694420709264534
1106	36-37	0.05537610055792186
1106	38-39	0.009426244680959428
1106	40-41	0.06135315664475627
1106	42-43	0.035325048361222855
1106	44-45	-0.0740632670594934
1106	46-47	0.037842908508174844
1106	48-49	-0.033109902189870866
1106	50-51	-0.052029929544659126
1106	52-53	-0.06678085388416122
1106	54-55	0.005642917563186245
1106	56-57	-0.01793100505628331
1106	58-59	-0.012952255437973292
1106	60-61	-0.01885651207764738
1106	62-63	-0.009562866038272944
1106	64-65	0.019176523219407215
1106	66-67	0.0215751321979738
1106	68-69	0.08033609807348085
1106	70-71	0.04629539045905773
1106	72-73	0.06456065073566464
1106	74-75	0.10236857099961227
1106	76-77	-0.010337013541153794
1106	78-79	-0.056242675571049006
1106	80-81	-0.021657804178342843
1106	82-83	0.046203858779119145
1106	84-85	0.03311756514088415
1106	86-87	-0.04385342913450785
1106	88-89	0.06720173540383101
1106	90-91	0.04653432915318234
1106	92-93	0.03975269344304522
1106	94-95	0.03252364825203102
1106	96-97	-0.07060156463616707
1106	98-99	-0.014019433445628948
1106	100	-0.00630241602000936
1107	1	-0.014037029545868052
1107	2	-0.10704896255482765
1107	3	-0.09974079837175864
1107	4	0.07757149239832728
1107	5	0.03663421761985575
1107	6	-0.012003790455487717
1107	7	0.11622077835121303
1107	8	0.14721476536533373
1107	9	0.12282841110911846
1107	10-11	0.098536403286964
1107	12-13	0.13237279207273644
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2314	100	0.5369983133963387
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2316	80-81	-0.08083771814148477
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2316	88-89	0.05747531454931121
2316	90-91	0.08820561207166122
2316	92-93	0.22981911014597145
2316	94-95	0.33132528144110296
2316	96-97	0.3545159000818572
2316	98-99	0.2993791440713558
2316	100	-0.040363631948551415
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	21136.0
1	1880.0
2	1316.0
3	915.0
4	797.0
5	750.0
6	872.0
7	2174.0
8	4357.0
9	6172.0
10	10685.0
11	20205.0
12	32421.0
13	41261.0
14	47437.0
15	52904.0
16	60188.0
17	69130.0
18	81505.0
19	94310.0
20	107552.0
21	123753.0
22	138715.0
23	150807.0
24	160183.0
25	171175.0
26	186696.0
27	209839.0
28	240561.0
29	282737.0
30	337037.0
31	412484.0
32	521956.0
33	686207.0
34	976043.0
35	1529326.0
36	1566253.0
37	1558645.0
38	1370679.0
39	280185.0
40	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.39074642361944	17.42804296065728	12.35229339742662	23.82891721829666
2	16.386275572395725	19.930752414434387	39.43042553889455	24.252546474275334
3	19.732493532512706	25.369148946785476	28.913483827051817	25.984873693649995
4	13.188325293623798	15.875884099075321	36.41856536042644	34.51722524687444
5	13.8409363122605	36.4506374118832	33.74806204644425	15.960364229412052
6	31.662134835182925	34.76466881611402	18.667202727667078	14.905993621035977
7	28.770936833372733	31.355414806390495	21.54659867137213	18.327049688864637
8	27.021208510461165	33.644280483764945	20.195894276581317	19.138616729192577
9	27.538259242725843	15.683863229695785	19.942698842207506	36.83517868537087
10-11	25.637823094295737	25.263350576937494	27.86956605246127	21.2292602763055
12-13	26.190467312055965	23.081939130228164	27.687982192680565	23.039611365035306
14-15	23.61243675042376	24.534094797701492	25.75225629855803	26.101212153316723
16-17	23.126846476387538	27.16998787292553	26.465860614120807	23.237305036566124
18-19	22.965784393756294	26.522680523181663	27.99378479074854	22.517750292313504
20-21	23.780333350879364	25.771518768514927	27.874939524729193	22.573208355876513
22-23	23.496726650684842	25.607423701839373	27.61585487089171	23.27999477658408
24-25	23.301772064309898	25.70681186671343	27.68325342214827	23.308162646828404
26-27	23.17828961644038	25.631461855933356	27.90524175673388	23.28500677089238
28-29	23.025944987509025	26.08601582772536	27.867093448669632	23.020945736095978
30-31	23.686761230441782	25.750774854811453	27.730975750449716	22.83148816429705
32-33	23.295393845048164	25.868419035369953	27.81617367530902	23.020013444272866
34-35	23.539857451401268	25.939476972688574	27.669452572423303	22.851213003486855
36-37	23.630108494286116	25.816893678582375	27.615359746756056	22.937638080375457
38-39	23.46127411210582	26.101309701446755	27.465764313916473	22.971651872530956
40-41	23.41749276520112	25.950254521562293	27.583662382961517	23.04859033027507
42-43	23.254601184834442	26.031776670253542	27.278458985971483	23.43516315894054
44-45	23.305187389544415	25.999007460346967	27.360047400531016	23.3357577495776
46-47	23.319024136565773	25.95839271173852	27.280823293464312	23.441759858231396
48-49	23.402627377371758	26.034257894614075	27.232587054430983	23.33052767358318
50-51	23.013019219921947	26.329109402224667	27.374783687493288	23.283087690360098
52-53	23.150367257089762	26.468353026942566	26.886677364736617	23.49460235123106
54-55	23.027508681226724	26.23012539291937	27.148899847457063	23.593466078396844
56-57	23.25627057204369	26.20968180807241	26.940931818642056	23.593115801241847
58-59	23.362887176293366	26.074857295710846	27.15004521901444	23.412210308981347
60-61	23.424058094274958	26.223957532885233	27.29426696465139	23.05771740818842
62-63	23.06869805486351	26.315090611125473	27.29907905138186	23.317132282629156
64-65	23.034109933456143	26.2402852354137	27.13844498637647	23.587159844753693
66-67	23.14279064727134	26.093111946272707	26.949290623616285	23.81480678283967
68-69	22.923828757704527	26.148154187278212	27.069553576423417	23.858463478593848
70-71	23.028227588314014	25.989642458528976	27.16675055341834	23.815379399738674
72-73	23.01378845412985	26.117681782341005	27.05204550288333	23.81648426064582
74-75	22.954807534551083	26.379260073578116	26.86777428648366	23.79815810538714
76-77	22.89393529624935	25.985763079278602	27.030130354327913	24.090171270144133
78-79	22.91448191812079	25.993312582369203	27.116393861585347	23.97581163792466
80-81	22.95555132630894	26.20320946049224	26.796359130335652	24.04488008286317
82-83	22.94631600781346	26.074974122023313	26.96046082812214	24.01824904204108
84-85	22.903662610891068	26.27348287479731	26.769103484623958	24.053751029687664
86-87	22.797323868025586	26.17295442439055	26.920790294275033	24.108931413308834
88-89	22.675203573790757	25.959213679291317	26.979327095102303	24.386255651815627
90-91	23.12871711370153	25.85313282197168	26.900706456249868	24.117443608076922
92-93	22.96334761753682	25.9650075822094	26.929732238067977	24.1419125621858
94-95	22.775846936695093	25.701303566022077	27.068289032894448	24.454560464388376
96-97	22.74493279652316	25.62577570079029	27.235308490495914	24.39398301219064
98-99	22.849600416215672	25.647872885277344	26.934562287756957	24.567964410750022
100	22.870596497644268	25.382324409228058	27.06570120111823	24.68137789200944
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8275.0
1	9775.0
2	10083.5
3	6666.5
4	3580.5
5	2891.0
6	3749.0
7	6030.5
8	9160.5
9	11043.5
10	10436.0
11	9411.5
12	9634.5
13	10280.0
14	10949.5
15	11682.0
16	12498.0
17	13295.5
18	14205.0
19	15363.5
20	16644.5
21	18378.0
22	20759.5
23	23966.5
24	28326.5
25	33974.0
26	41312.0
27	50528.5
28	60678.5
29	73642.5
30	90399.0
31	107282.5
32	126863.5
33	149401.0
34	172276.0
35	193510.0
36	219249.0
37	246296.5
38	266328.5
39	285045.0
40	300691.0
41	313932.5
42	328657.5
43	347416.0
44	366111.0
45	384708.0
46	405539.5
47	435424.0
48	481090.0
49	505942.0
50	513832.0
51	501801.0
52	469333.5
53	452835.0
54	441157.0
55	419237.0
56	384143.5
57	347637.5
58	314007.5
59	276789.5
60	237028.0
61	198424.0
62	163615.0
63	130548.0
64	99217.5
65	75144.0
66	57307.5
67	44947.5
68	35536.0
69	27243.5
70	21512.0
71	17157.0
72	13558.5
73	10671.0
74	8362.5
75	6336.5
76	4494.5
77	3055.0
78	2059.5
79	1391.5
80	989.0
81	704.5
82	495.5
83	361.0
84	292.5
85	230.5
86	177.5
87	134.0
88	101.0
89	78.5
90	63.5
91	58.5
92	43.0
93	32.0
94	29.0
95	24.0
96	19.0
97	15.0
98	11.5
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14767120102796386
2	0.07065694612531112
3	0.31605771433039465
4	0.31314286663248425
5	0.31528791775735593
6	0.26354720876952253
7	0.26382398955982855
8	0.26186922522829226
9	0.2727761182462892
10-11	0.25089746171256727
12-13	0.25998365609433244
14-15	0.23551450435133997
16-17	0.2440125395537048
18-19	0.2576180452771856
20-21	0.25881598713522885
22-23	0.24921515347148845
24-25	0.24997630064483006
26-27	0.24800856221374812
28-29	0.25748830428172964
30-31	0.24257241450414374
32-33	0.25470752227912374
34-35	0.23876667863743586
36-37	0.24723876564070948
38-39	0.24935786856649
40-41	0.26761675132699086
42-43	0.2522813656640974
44-45	0.2374087228849969
46-47	0.24020680368699693
48-49	0.2495784282587651
50-51	0.24326004178006033
52-53	0.2606280363717637
54-55	0.2707218858182367
56-57	0.2666566679606169
58-59	0.26290282849209134
60-61	0.2442806709443138
62-63	0.2723047259627993
64-65	0.26406184805149785
66-67	0.26979640005065086
68-69	0.27462708978146083
70-71	0.2945120596850096
72-73	0.3040220746519309
74-75	0.28249371880594
76-77	0.28074221536728466
78-79	0.2787917757355968
80-81	0.26535925800605736
82-83	0.27595477263496004
84-85	0.2864810920662862
86-87	0.2775981585774021
88-89	0.28647676736643773
90-91	0.2808762810625891
92-93	0.2614410799432876
94-95	0.28281374659473135
96-97	0.2802837971833403
98-99	0.2675735043285056
100	0.2910955468046696
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1561496E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.10649691428863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.79476413622865	38.304566872846465
2	16.516179656558684	17.87265247246858
3	5.95224451399092	9.661652982880234
4	2.54202840521575	5.501610082513601
5	1.3132986295903422	3.552899412473466
6	0.769119326021103	2.496861146404833
7	0.4615106780520516	1.7479508254554206
8	0.3145959905020236	1.361734959147625
9	0.2265344062440175	1.1031284837179922
>10	1.0145730416777687	9.556263967674264
>50	0.059505556359459234	2.205156240054934
>100	0.03261937319324225	3.3192823597622696
>500	0.001871432434518112	0.6859675624200251
>1k	9.700773202701299E-4	1.0129459334957367
>5k	6.929122919422162E-5	0.2600379698666152
>10k+	1.1548538199036936E-4	1.3572887288179167
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32301	0.2793842596148457	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26889	0.23257370845433845	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	19614	0.16964932565820204	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15695	0.13575232824541045	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	14110	0.12204302972556494	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.649399697063426E-6
2	0.0	0.0	0.0	0.0	8.649399697063426E-6
3	0.0	0.0	0.0	0.0	8.649399697063426E-6
4	0.0	0.0	0.0	8.649399697063426E-6	2.5948199091190273E-5
5	0.0	0.0	0.0	8.649399697063426E-6	2.5948199091190273E-5
6	8.649399697063426E-6	0.0	0.0	8.649399697063426E-6	6.0545797879443976E-5
7	8.649399697063426E-6	0.0	0.0	8.649399697063426E-6	6.0545797879443976E-5
8	8.649399697063426E-6	0.0	0.0	8.649399697063426E-6	7.784459727357082E-5
9	8.649399697063426E-6	0.0	0.0	8.649399697063426E-6	1.037927963647611E-4
10-11	8.649399697063426E-6	0.0	0.0	1.2974099545595138E-5	1.037927963647611E-4
12-13	8.649399697063426E-6	4.324699848531713E-6	0.0	3.45975987882537E-5	1.0811749621329281E-4
14-15	8.649399697063426E-6	8.649399697063426E-6	0.0	3.45975987882537E-5	1.816373936383319E-4
16-17	8.649399697063426E-6	8.649399697063426E-6	0.0	4.3246998485317125E-5	2.5083259121483933E-4
18-19	8.649399697063426E-6	8.649399697063426E-6	0.0	8.649399697063425E-5	2.767807903060296E-4
20-21	8.649399697063426E-6	8.649399697063426E-6	0.0	1.2974099545595138E-4	2.8110549015456133E-4
22-23	8.649399697063426E-6	8.649399697063426E-6	0.0	2.681313906089662E-4	3.0705368924575157E-4
24-25	1.2974099545595138E-5	8.649399697063426E-6	0.0	6.141073784915031E-4	3.6327478727666386E-4
26-27	1.729879939412685E-5	8.649399697063426E-6	0.0	0.0013276828534992359	4.021970859134493E-4
28-29	1.729879939412685E-5	8.649399697063426E-6	0.0	0.004164685954136039	4.06521785761981E-4
30-31	1.729879939412685E-5	8.649399697063426E-6	0.0	0.013328724933174737	4.108464856105127E-4
32-33	1.729879939412685E-5	8.649399697063426E-6	0.0	0.03653938902024444	4.7139228348995666E-4
34-35	1.729879939412685E-5	1.729879939412685E-5	0.0	0.07491245077626632	5.016651824296787E-4
36-37	1.729879939412685E-5	1.729879939412685E-5	0.0	0.1352549877628293	5.319380813694006E-4
38-39	1.729879939412685E-5	1.729879939412685E-5	0.0	0.2252865892095625	5.57886280460591E-4
40-41	1.729879939412685E-5	1.729879939412685E-5	0.0	0.3287593577855322	5.622109803091227E-4
42-43	1.729879939412685E-5	1.729879939412685E-5	0.0	0.42689544674841384	5.751850798547179E-4
44-45	1.729879939412685E-5	1.729879939412685E-5	0.0	0.5489687493729185	5.838344795517812E-4
46-47	3.45975987882537E-5	1.729879939412685E-5	0.0	0.6972929800780108	6.530296771282886E-4
48-49	3.45975987882537E-5	1.729879939412685E-5	0.0	0.8511268783901322	7.179001748562643E-4
50-51	3.45975987882537E-5	1.729879939412685E-5	0.0	1.0117289319652059	7.265495745533277E-4
52-53	3.45975987882537E-5	1.729879939412685E-5	0.0	1.1737278635913553	8.087188716754302E-4
54-55	3.45975987882537E-5	1.729879939412685E-5	0.0	1.3577351927466825	8.952128686460644E-4
56-57	3.45975987882537E-5	1.729879939412685E-5	0.0	1.5723354486305232	9.254857675857865E-4
58-59	3.45975987882537E-5	1.729879939412685E-5	0.0	1.8086024507555076	9.600833663740402E-4
60-61	3.45975987882537E-5	1.729879939412685E-5	0.0	2.0522733390211787	9.860315654652305E-4
62-63	3.45975987882537E-5	1.729879939412685E-5	0.0	2.301099269506299	0.001037927963647611
64-65	3.892229863678542E-5	1.729879939412685E-5	0.0	2.5591627588678834	0.001037927963647611
66-67	4.3246998485317125E-5	1.729879939412685E-5	0.0	2.8600191532306893	0.0010422526634961426
68-69	6.91951975765074E-5	1.729879939412685E-5	0.0	3.1977911855005616	0.0010552267630417377
70-71	6.91951975765074E-5	1.729879939412685E-5	0.0	3.559496106732208	0.0011157725609211818
72-73	9.081869681916596E-5	1.729879939412685E-5	0.0	3.9060170067956603	0.0012541629560741967
74-75	9.514339666769768E-5	1.729879939412685E-5	0.0	4.255608443751569	0.0012714617554683235
76-77	9.514339666769768E-5	3.45975987882537E-5	0.0	4.637051295091915	0.0012930852547109821
78-79	9.514339666769768E-5	3.45975987882537E-5	8.649399697063426E-6	5.06527009999398	0.0013147087539536406
80-81	1.037927963647611E-4	3.45975987882537E-5	8.649399697063426E-6	5.51526809333325	0.001340656953044831
82-83	1.1244219606182452E-4	3.892229863678542E-5	8.649399697063426E-6	5.9712774194619795	0.0014530991491066554
84-85	1.1244219606182452E-4	4.3246998485317125E-5	8.649399697063426E-6	6.4312568200516615	0.0014530991491066554
86-87	1.2974099545595138E-4	4.3246998485317125E-5	8.649399697063426E-6	6.899868321539012	0.0014617485488037187
88	1.2974099545595138E-4	4.3246998485317125E-5	8.649399697063426E-6	7.2775184111121956	0.0014703979485007823
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	19680	0.0	51.300987	2
GTACCTG	22575	0.0	45.23841	1
ACCTGGG	22870	0.0	43.775593	3
CCTGGGG	20525	0.0	32.323986	4
GTACCCG	2055	0.0	24.493294	1
CTGGGGG	13515	0.0	22.577984	5
CATGGGG	29280	0.0	22.160986	4
GTACATG	57385	0.0	21.764135	1
TACATGG	58310	0.0	21.1531	2
ACATGGG	58825	0.0	20.400522	3
TACCCGG	2480	0.0	19.909319	2
TGGGGGG	16695	0.0	19.788483	6
GAGTACT	29735	0.0	19.343264	12-13
ATGGGGG	17365	0.0	18.926004	5
GTATCAA	70655	0.0	18.39557	1
TATAACG	1095	0.0	17.607126	2
GTACTTT	33360	0.0	17.213566	14-15
AGTACTT	31520	0.0	16.943357	12-13
GTATAGG	2920	0.0	16.915379	1
TATCACG	1245	0.0	16.618893	2
>>END_MODULE