FastQCFastQC Report
Mon 6 Jun 2016
ERR522855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522855_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11561496
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293700.2540328691027528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262010.2266229214627588No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT205740.1779527493673829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154620.13373701811599467No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA141350.1222592647179915No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG203400.052.034512
GTACCTG233650.045.8234021
ACCTGGG231600.045.0448653
CCTGGGG202050.034.8404544
CTGGGGG122250.024.409325
TATAACG11800.023.100432
CATGGGG282900.020.7638514
TGGGGGG151050.020.626416
GTACCCG21350.020.4821321
GTACATG580900.019.799091
GAGTACT302700.019.3487512-13
ATAACGC14100.019.330353
TACATGG594350.019.11212
ATGGGGG162450.018.8028855
ACATGGG588800.018.62793
TACCCGG22550.018.548872
TAACGCA14900.017.977094
AGTACTT319100.017.8534912-13
TACCGTA7800.017.4717397
GTATCAA706650.017.4535621
Produced by FastQC (version 0.11.5)