FastQCFastQC Report
Mon 6 Jun 2016
ERR522854_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522854_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4883060
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159880.3274176438544683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128390.26292939263494614No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104470.2139437156209426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71020.1454415878567947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG72050.047.9739232
ACCTGGG80000.041.7364773
TATAACG5000.038.562552
GTACCTG94150.037.0618021
ATAACGC5800.032.4324263
TAACGCA6400.032.3310744
CCTGGGG74300.029.5580334
GTACATG175450.024.2034551
TACATGG178300.023.2895582
CATGGGG107600.022.4645794
ACATGGG179450.022.4325033
GTATAAC13850.021.7306731
TATCACG4250.021.024062
GTATCAA288250.020.93151
GAGTACT149800.020.14235112-13
CTGGGGG51350.019.4146465
GGTATCA224000.018.999561
TCAACGC316050.018.852474
CAACGCA317100.018.7894675
GTACACG10800.018.7235281
Produced by FastQC (version 0.11.5)