FastQCFastQC Report
Mon 6 Jun 2016
ERR522854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522854_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4883060
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147830.3027404946898052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126770.25961180079704116No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT107920.22100895749796234No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT73500.15052037042346397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67580.13839682494173736No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG75400.049.2495652
ACCTGGG80400.044.7233433
GTACCTG98500.038.1048551
CCTGGGG76650.032.25544
TATAACG6100.031.5937182
TAACGCA6850.028.1333984
ATAACGC7500.026.9485933
CTGGGGG48900.022.396975
GTACATG169500.022.0047861
TACATGG171450.021.16542
GTATCAA280350.020.6020981
CATGGGG99400.020.5225934
GTATAAC15100.020.2465061
ACATGGG171550.020.2206233
GAGTACT151400.019.9903312-13
GGTATCA219050.019.3032341
TCAACGC308050.018.5694184
TGGGGGG61300.018.5540756
CAACGCA309650.018.4740355
GTACTTT163950.018.18752114-15
Produced by FastQC (version 0.11.5)