FastQCFastQC Report
Mon 6 Jun 2016
ERR522853_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522853_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6058653
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT206480.34080182509214507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186790.3083028521356149No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158900.2622695176634146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87280.1440584235472802No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG217000.026.9538751
TACATGG222700.025.7342592
ACATGGG230700.023.9465853
CATGGGG166450.022.1154254
GAGTACT167900.020.70805712-13
ATGGGGG98600.020.645985
AGAGTAC268050.019.24507910-11
GTACTTT181400.018.894914-15
GTATCAA399500.018.8423521
AGTACTT176350.018.15719412-13
TCAACGC431350.017.0896664
CAACGCA434900.016.9504495
GGTATCA319450.016.7347071
TATACTG26450.016.7080885
AACGCAG442750.016.6708586
ATCAACG442400.016.6323093
GAGTACA171100.016.6251621
TATCAAC446650.016.5888792
ACTTTTT220300.015.8566816-17
AGTACAT167700.015.7274362
Produced by FastQC (version 0.11.5)