FastQCFastQC Report
Mon 6 Jun 2016
ERR522853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522853_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6058653
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT202370.3340181390153884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164600.2716775494486976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143880.23747852864324792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79900.131877498183177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG218200.025.9974371
TACATGG224300.025.042782
ACATGGG230500.023.6709443
CATGGGG159200.022.6710914
ATGGGGG95650.020.340545
GAGTACT169450.020.0576512-13
AGAGTAC269950.018.64167210-11
GTACTTT181800.018.51187914-15
ATAACGC5800.017.8257983
GTATCAA410200.017.567141
AGTACTT179450.017.1061812-13
GAGTACA171950.016.5223831
TGGGGGG85600.016.1406366
TCAACGC441550.016.0287384
GGTATCA334000.016.0263161
CAACGCA445350.015.9022625
TATACTG24100.015.7948355
ATCAACG450750.015.7015863
TATCAAC454150.015.6804022
AACGCAG454900.015.5994086
Produced by FastQC (version 0.11.5)