FastQCFastQC Report
Mon 6 Jun 2016
ERR522852_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522852_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5965105
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT369860.6200393790218278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203810.3416704316185549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161460.27067419601163767No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT103830.1740623174277737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95990.16091921265426176No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC77890.13057607535827115No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC72460.12147313416947397No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG46100.029.8802742
GTACATG213350.028.539251
TACATGG216350.027.6623692
ACATGGG220600.026.3637813
CATGGGG149600.024.2288824
GTACCTG59800.023.9808221
ATGGGGG91450.022.9795595
ACCTGGG62950.021.509993
GAGTACT179800.020.19815312-13
AGAGTAC272750.019.05760210-11
GTACTTT196350.018.31630914-15
TATAACG4450.017.9600182
AGTACTT186700.017.57696512-13
ATAACGC6150.016.8186473
TGGGGGG90950.016.7875696
GAGTACA161800.016.6801051
CATGGGA79000.016.5435334
GTATTAC13300.015.5548721
AGTACAT159800.015.3866052
CGTATAC4602.2009772E-1015.3312263
Produced by FastQC (version 0.11.5)