FastQCFastQC Report
Mon 6 Jun 2016
ERR522852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5965105
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT375760.6299302359304656No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189690.31799943169483186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156710.2627112179919716No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT102750.17225178768856542No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92200.15456559440278084No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC81200.1361250137256595No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC77600.13008991459496522No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG61050.10234522275802355No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA60020.10061851384007492No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG201150.027.403771
TACATGG204850.026.2964082
TACCTGG46350.025.961992
ACATGGG212700.024.8347193
CATGGGG141950.022.8772094
ATGGGGG85850.021.3489955
GTACCTG58300.021.217291
TATCACG5200.020.7908522
GAGTACT180650.020.24690412-13
ACCTGGG60100.019.5490633
AGAGTAC269650.018.80317710-11
GTACTTT200350.018.15885514-15
AGTACTT190450.018.14359512-13
TAACGCA5300.017.734324
GAGTACA161150.017.6866281
AGTACAT155800.016.8048842
TAGGTCG4005.0386006E-1016.4483075
GTACCGT6550.015.7846346
CATGGGA77300.015.7463784
ACTTTTT241050.015.27558116-17
Produced by FastQC (version 0.11.5)