FastQCFastQC Report
Mon 6 Jun 2016
ERR522851_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522851_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4186108
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT311340.7437457418681028No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146990.3511376199562935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120260.28728355790151616No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT86200.20591919749801008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68730.16418592162457346No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC66370.15854822665827065No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC59300.14165903029735497No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA49150.11741216423465425No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG45500.1086928478672791No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA45320.10826285418340856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG35350.033.647472
GTACATG152500.030.3050771
ATGGGGG77650.029.182095
TACATGG156300.029.1765582
CATGGGG111300.028.1728734
ACATGGG159700.027.7916563
GTACCTG45850.027.3781191
ACCTGGG43100.026.835223
TGGGGGG83950.023.4508916
GGGTTAG19550.021.4030171
GAGTACT126650.021.2391112-13
TATCACG3850.020.7591462
CGGTTAA12500.019.1814522
GTACTTT144600.018.4075114-15
AGAGTAC192400.018.36283710-11
TATAACG3651.364242E-1018.0325222
GTGTAGC21500.017.7124351
AGTACTT134050.017.6831812-13
ATAACGC4053.274181E-1117.41343
GCGGTTA14900.017.3543421
Produced by FastQC (version 0.11.5)