FastQCFastQC Report
Mon 6 Jun 2016
ERR522851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522851_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4186108
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT304890.7283376348627413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129890.31028821998859085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108150.25835453839222494No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT81970.19581434592705205No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC67960.16234650419912722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62210.14861059485326225No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA49110.11731661008268301No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG46920.11208502026225793No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA45300.10821507710742292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG36750.035.811232
GTACCTG46500.029.2351971
GTACATG157750.028.715611
ACCTGGG46300.027.709433
TACATGG162300.027.6279342
ACATGGG161200.027.112143
CATGGGG115200.026.5975154
ATGGGGG83600.026.5327135
TAACGCA4100.022.9240884
TATAACG4350.022.6907372
TGGGGGG87200.022.5271226
CCTGGGG42800.020.422794
GAGTACT132550.020.39315812-13
GGGTTAG17550.019.8342481
ATAACGC4200.019.0215363
AGAGTAC203850.018.87575510-11
TATCACG4001.8189894E-1218.8008962
GTACTTT145700.018.46917314-15
AGTACTT139600.018.23515512-13
GAGTACA121350.017.2496911
Produced by FastQC (version 0.11.5)