FastQCFastQC Report
Mon 6 Jun 2016
ERR522850_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522850_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7494621
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308440.41154849591460324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249780.33327902771867984No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT232470.3101824628623649No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159630.212992758406329No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148280.19784856365652115No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA99420.1326551402665992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG168700.060.2886772
GTACCTG190050.053.802841
ACCTGGG186800.053.6166843
TATAACG8800.043.8152732
CCTGGGG167100.041.4741554
ATAACGC11000.036.762333
TAACGCA13200.031.3447824
CTGGGGG98100.030.8634385
GTACCCG18550.029.1586531
GTATAAC18900.026.3789581
TACCCGG20650.025.9584732
TGGGGGG136850.024.8626146
TATCACG7600.024.7480352
CATGGGG192350.023.8569074
ATGGGGG121300.022.4799145
GTACATG378750.022.0051671
GTACACG17650.021.3186071
TACATGG387050.021.1264652
ACATGGG387950.020.5442923
GAGTACT265900.019.47367912-13
Produced by FastQC (version 0.11.5)