FastQCFastQC Report
Mon 6 Jun 2016
ERR522850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7494621
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278640.371786645382068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246290.3286223546193997No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT242110.32304502122255413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154810.20656147922623438No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130090.1735778233482387No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA98390.13128082127168272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG176600.059.454692
ACCTGGG192400.053.6130943
GTACCTG198300.053.2549931
CCTGGGG158900.042.6760184
TATAACG9250.036.5834272
CTGGGGG91850.033.0006145
GTACCCG18250.032.9777031
ATAACGC10850.030.7517663
TAACGCA11650.029.0434384
TACCCGG21600.026.7636282
TGGGGGG120650.025.2399886
TACCGTA6900.025.1997937
GTACCGT11000.023.069736
GTATAAC19800.022.7970871
CCGTATG9550.021.6582859
ACCGTAT8750.021.4896628
GTACCGG13250.021.2916181
CATGGGG190200.021.1497234
GTACATG390300.019.829171
ATGGGGG113850.019.7716965
Produced by FastQC (version 0.11.5)