##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522850_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7494621
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.215724717767586	33.0	31.0	34.0	30.0	34.0
2	32.32981761185789	34.0	31.0	34.0	30.0	34.0
3	32.525870487647076	34.0	31.0	34.0	30.0	35.0
4	35.621378986342336	37.0	35.0	37.0	33.0	37.0
5	35.5621288921748	36.0	35.0	37.0	33.0	37.0
6	35.68729973136734	37.0	35.0	37.0	33.0	37.0
7	35.53304309851025	36.0	35.0	37.0	33.0	37.0
8	35.519921020689374	36.0	35.0	37.0	33.0	37.0
9	36.80946828398661	38.0	37.0	39.0	33.0	39.0
10-11	36.60223745803824	37.5	35.0	39.0	33.0	39.0
12-13	36.60951801298558	37.5	35.0	39.0	33.0	39.0
14-15	37.76820148744012	38.0	37.0	41.0	33.0	41.0
16-17	37.59108679411541	38.0	36.5	41.0	33.0	41.0
18-19	37.4982744290872	38.0	36.0	41.0	32.0	41.0
20-21	37.51136314965093	38.0	36.5	41.0	33.0	41.0
22-23	37.36086514581591	38.0	36.0	41.0	33.0	41.0
24-25	37.28475349187104	38.0	36.5	40.5	32.0	41.0
26-27	36.96174796564096	38.0	36.0	40.0	32.5	41.0
28-29	36.924409786699016	38.0	36.0	40.0	32.0	41.0
30-31	36.67744012672556	38.0	35.5	40.0	31.5	41.0
32-33	36.613840913369735	37.5	36.0	40.0	30.5	41.0
34-35	36.3647566701505	37.5	35.0	40.0	30.5	41.0
36-37	36.137437036509255	37.0	35.0	40.0	30.0	41.0
38-39	36.08771817814403	37.0	35.0	40.0	30.0	41.0
40-41	36.283013710766696	37.5	35.0	40.0	30.0	41.0
42-43	36.31871471552731	37.5	35.5	40.0	30.5	41.0
44-45	36.13233297587696	37.0	35.0	40.0	30.0	41.0
46-47	36.01529136696839	37.0	35.5	40.0	29.5	41.0
48-49	35.78700650773401	37.5	35.0	40.0	29.5	41.0
50-51	35.61518641169447	37.5	34.5	40.0	28.0	41.0
52-53	35.62996961420731	37.5	35.0	40.0	28.5	41.0
54-55	35.43217735760087	37.5	34.0	40.0	27.0	41.0
56-57	35.176480905438716	37.0	34.5	40.0	27.5	41.0
58-59	34.98564530481261	37.0	34.0	39.5	27.0	41.0
60-61	34.78225590059857	36.5	34.0	39.0	26.5	41.0
62-63	34.535991279612404	36.5	34.0	39.0	26.0	41.0
64-65	34.352853933507774	36.5	33.5	38.5	25.5	40.5
66-67	33.84986518998092	35.5	33.5	38.0	26.0	40.0
68-69	33.70392345390114	35.5	33.0	37.0	25.0	40.0
70-71	33.279880396887314	35.0	32.5	37.0	24.5	39.5
72-73	33.04723514371174	35.0	32.5	37.0	24.0	39.0
74-75	32.85966401770016	35.0	32.0	37.0	23.5	39.0
76-77	31.271416326456	34.0	30.0	36.0	22.0	37.0
78-79	32.38805244454656	35.0	31.5	36.5	23.0	37.0
80-81	32.49874356555188	35.0	32.0	36.0	24.0	37.5
82-83	32.40319437100288	35.0	32.0	37.0	23.5	37.0
84-85	32.076681195753594	35.0	32.0	36.0	23.0	36.5
86-87	31.9638647504657	35.0	32.0	36.0	23.0	36.0
88-89	31.926464460310935	35.0	32.0	36.0	22.5	37.0
90-91	31.69243648211164	34.5	32.0	35.0	23.0	36.5
92-93	31.47937941091351	34.5	31.0	35.0	21.5	36.5
94-95	31.61587977564176	34.0	31.0	35.0	21.0	36.5
96-97	31.346114499986058	34.0	31.0	35.0	21.0	37.0
98-99	31.20401658736312	34.0	31.0	35.0	20.0	36.0
100	29.97956681198422	34.0	29.0	35.0	18.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.10715628278165212
1101	2	-0.15782066362016423
1101	3	-0.15911984618717412
1101	4	-0.09992691292169553
1101	5	-0.1854959141778849
1101	6	-0.11546308788775406
1101	7	-0.09401461878375983
1101	8	-0.18188598479949292
1101	9	-0.15630591131782268
1101	10-11	-0.16046529277389254
1101	12-13	-0.11457909051873116
1101	14-15	-0.3145300649249023
1101	16-17	-0.3270485342378038
1101	18-19	-0.4347782301240173
1101	20-21	-0.3171763058824766
1101	22-23	-0.8488771018368624
1101	24-25	-0.32740096409518316
1101	26-27	-0.28760822451250334
1101	28-29	-0.3099590256506559
1101	30-31	-0.3224745810619609
1101	32-33	-0.3025618642508263
1101	34-35	-0.34680574099616024
1101	36-37	-0.4684715568631006
1101	38-39	-0.39857044807760644
1101	40-41	-0.3442957751999032
1101	42-43	-0.30355226744060104
1101	44-45	-0.36166203989913726
1101	46-47	-0.2587952048447093
1101	48-49	-0.3383154570671323
1101	50-51	-0.2743337011551503
1101	52-53	-0.3315598993944704
1101	54-55	-0.28767914652353
1101	56-57	-0.39615045501596313
1101	58-59	-0.37370955284002605
1101	60-61	-0.5830614202884519
1101	62-63	-0.4791049032121961
1101	64-65	-0.43918469362941437
1101	66-67	-0.4056675601621791
1101	68-69	-0.35527144288740686
1101	70-71	-0.545053334235547
1101	72-73	-0.538654448677633
1101	74-75	-0.39193361345637356
1101	76-77	-0.5959842819251016
1101	78-79	-0.49355869603158453
1101	80-81	-0.3537576844680359
1101	82-83	-0.27317672543903626
1101	84-85	-0.33947935634038373
1101	86-87	-0.4914498115474224
1101	88-89	-0.4954305065902709
1101	90-91	-0.5502951788698738
1101	92-93	-0.4695407115921384
1101	94-95	-0.5571614353414276
1101	96-97	-0.6909139714246493
1101	98-99	-0.5805583944550641
1101	100	-0.5654610201470547
1102	1	-0.09158177121739897
1102	2	-0.10446943084254912
1102	3	-0.13928860438526414
1102	4	-0.11415232845450163
1102	5	-0.15625747027353043
1102	6	-0.22990227126290108
1102	7	-0.18368770221474762
1102	8	-0.1820168744857824
1102	9	-0.1335198255254184
1102	10-11	-0.30486684070292114
1102	12-13	-0.21106622203024017
1102	14-15	-0.24383170691923084
1102	16-17	-0.3069636983300299
1102	18-19	-0.2953998256170891
1102	20-21	-0.4173352939672199
1102	22-23	-0.3216314534061908
1102	24-25	-0.29654892257965315
1102	26-27	-0.2811998785237009
1102	28-29	-0.4100166021964142
1102	30-31	-0.41931775302993657
1102	32-33	-0.3347362178897697
1102	34-35	-0.34093891845516566
1102	36-37	-0.5004144296679769
1102	38-39	-0.4756257191376463
1102	40-41	-0.41730318016271184
1102	42-43	-0.4381127195507304
1102	44-45	-0.30590871102201334
1102	46-47	-0.3356168695116679
1102	48-49	-0.40316404361700364
1102	50-51	-0.29208496227354885
1102	52-53	-0.41186245593852533
1102	54-55	-0.3770740614032775
1102	56-57	-0.33929797906601067
1102	58-59	-0.41938506574766876
1102	60-61	-0.5277027928029199
1102	62-63	-0.5930804257221425
1102	64-65	-0.5075178451715203
1102	66-67	-0.5618864144299422
1102	68-69	-0.3937853322035849
1102	70-71	-0.46609072822562325
1102	72-73	-0.5028172907364805
1102	74-75	-0.5183800889905399
1102	76-77	-0.48780805816712913
1102	78-79	-0.6000547959104807
1102	80-81	-0.47665113895823197
1102	82-83	-0.4290088678067434
1102	84-85	-0.4118183196082974
1102	86-87	-0.38861476331680933
1102	88-89	-0.45038237852720187
1102	90-91	-0.5271628693474604
1102	92-93	-0.5284631464013394
1102	94-95	-0.6252905705092644
1102	96-97	-0.6535812683009183
1102	98-99	-0.6613036545983171
1102	100	-0.868888801016837
1103	1	-0.1059117902105342
1103	2	-0.15718529805706538
1103	3	-0.2900823434604263
1103	4	-0.14155738168825138
1103	5	-0.20901987445656545
1103	6	-0.20574568760608258
1103	7	-0.12714051826886674
1103	8	-0.11951318557974133
1103	9	-0.1512456811624432
1103	10-11	-0.21528145482442795
1103	12-13	-0.2018473242375194
1103	14-15	-0.21820306057749406
1103	16-17	-0.2827644557854043
1103	18-19	-0.3450841876822679
1103	20-21	-0.3934909241837161
1103	22-23	-0.4341837034496976
1103	24-25	-0.41082453493806526
1103	26-27	-0.2946827544326922
1103	28-29	-0.2718980388645278
1103	30-31	-0.5104973895118974
1103	32-33	-0.36997807770377733
1103	34-35	-0.41844907713487345
1103	36-37	-0.5196528692039664
1103	38-39	-0.43819412469787977
1103	40-41	-0.39799809439091405
1103	42-43	-0.4445382452671325
1103	44-45	-0.31839628224627603
1103	46-47	-0.44997560521952806
1103	48-49	-0.34084080842749387
1103	50-51	-0.4374852747900988
1103	52-53	-0.4218462815043509
1103	54-55	-1.1406446386886984
1103	56-57	-0.4469705441988481
1103	58-59	-0.43199213861019103
1103	60-61	-0.4177336471913904
1103	62-63	-0.4196793111049999
1103	64-65	-0.41699386917400716
1103	66-67	-0.35526590638244926
1103	68-69	-0.40389178078968513
1103	70-71	-0.47392842392681445
1103	72-73	-0.5113044230755079
1103	74-75	-0.6167232972415846
1103	76-77	-0.4895964208982093
1103	78-79	-0.4370916963213709
1103	80-81	-1.28160853682283
1103	82-83	-0.40810270222593914
1103	84-85	-0.35482241334813125
1103	86-87	-0.43821573718800266
1103	88-89	-0.35723946841543963
1103	90-91	-0.4032826698816905
1103	92-93	-0.3103140313604378
1103	94-95	-0.4387039247274842
1103	96-97	-0.45703385734543645
1103	98-99	-0.4143539258747886
1103	100	-0.41529576869823615
1104	1	-0.030106262299277375
1104	2	-0.15164173643775314
1104	3	-0.09776011397208606
1104	4	-0.016166911225226954
1104	5	-0.02945114850096786
1104	6	-0.18067526098809594
1104	7	-0.14026113688161956
1104	8	-0.045367053877008345
1104	9	-0.09297869413902049
1104	10-11	-0.15601275842728768
1104	12-13	-0.08768775154941011
1104	14-15	-0.22967793198770892
1104	16-17	-0.26615714508491806
1104	18-19	-0.22033199129999304
1104	20-21	-0.2490440757786203
1104	22-23	-0.1659215399511993
1104	24-25	-0.14610835315718163
1104	26-27	-0.21953224738807364
1104	28-29	-0.33840645341100384
1104	30-31	-0.3528767721007071
1104	32-33	-0.22909767513807822
1104	34-35	-0.24456532175750567
1104	36-37	-0.3594422449528807
1104	38-39	-0.2656741997775427
1104	40-41	-0.12137066727097334
1104	42-43	-0.13653471973076847
1104	44-45	-0.23584544185801093
1104	46-47	-0.2698703062360792
1104	48-49	-0.3232714211615999
1104	50-51	-0.2309813525877118
1104	52-53	-0.2256065244544061
1104	54-55	-0.1688571716227898
1104	56-57	-0.19382378182567805
1104	58-59	-0.2553224652821555
1104	60-61	-0.2847628342107882
1104	62-63	-0.24322779657975246
1104	64-65	-0.5959089823447243
1104	66-67	-0.37862226567025203
1104	68-69	-0.287441183958272
1104	70-71	-0.2926779226497018
1104	72-73	-0.27524248009388685
1104	74-75	-0.48964019984018137
1104	76-77	-0.3531154665747316
1104	78-79	-0.2780089643453394
1104	80-81	-0.22054746497446587
1104	82-83	-0.30809339386669166
1104	84-85	-0.9649955375654251
1104	86-87	-0.4166754091252294
1104	88-89	-0.3770211398918626
1104	90-91	-0.33869354589029044
1104	92-93	-0.26358126170038787
1104	94-95	-0.28605070064827487
1104	96-97	-0.280184794399446
1104	98-99	-0.3604387188319471
1104	100	-1.4691321043873344
1105	1	-0.058864195056500535
1105	2	-0.17732361599487234
1105	3	-0.1744555298511017
1105	4	-0.0795133532992196
1105	5	-0.07338849659710434
1105	6	-0.10416044192246687
1105	7	-0.15222862945520887
1105	8	-0.1786728194724745
1105	9	-0.16785970278066742
1105	10-11	-0.1462339473281773
1105	12-13	-0.12213617174924707
1105	14-15	-0.19243363682162595
1105	16-17	-0.209928242843489
1105	18-19	-0.24818145457302165
1105	20-21	-0.28580227858715546
1105	22-23	-0.2776658182390861
1105	24-25	-0.1497937096249089
1105	26-27	-0.25583811425277503
1105	28-29	-0.22368739116418368
1105	30-31	-0.259002295325125
1105	32-33	-0.1685517495372153
1105	34-35	-0.26916235456874205
1105	36-37	-0.3406648178667311
1105	38-39	-0.2976486968069807
1105	40-41	-0.21732074153508307
1105	42-43	-0.23210081478882927
1105	44-45	-0.39220557215777063
1105	46-47	-0.37443628314668587
1105	48-49	-0.3561826586717203
1105	50-51	-0.19244081113483702
1105	52-53	-0.34101590536177895
1105	54-55	-1.1927584003562544
1105	56-57	-0.24279463316242555
1105	58-59	-0.17312517592217347
1105	60-61	-0.16648837768904912
1105	62-63	-0.1680632340628705
1105	64-65	-0.1443148357068651
1105	66-67	-0.33555794282390394
1105	68-69	-0.16965914912164948
1105	70-71	-0.12145526534481377
1105	72-73	-0.13111651803610158
1105	74-75	-0.2175297488544885
1105	76-77	-0.2428028821836925
1105	78-79	-0.20504869564428674
1105	80-81	-0.1592791521200212
1105	82-83	-0.14764240817941499
1105	84-85	-0.1943695248730144
1105	86-87	-0.1830687894924985
1105	88-89	-0.3207188174506008
1105	90-91	-0.3087039259875084
1105	92-93	-0.1593633978062492
1105	94-95	-0.2770347844992074
1105	96-97	-0.24710139917935692
1105	98-99	-0.08471675353354158
1105	100	-0.13260682083345898
1106	1	-0.027176349440637182
1106	2	-0.074064604963759
1106	3	-0.11454348497750999
1106	4	-0.05298512002173794
1106	5	-0.11868468974740409
1106	6	-0.04377382751397363
1106	7	-0.07130692878213551
1106	8	-0.1619866501114089
1106	9	-0.11938854365583751
1106	10-11	-0.17070087864533434
1106	12-13	-0.1777981958930397
1106	14-15	-0.17337506654902057
1106	16-17	-0.1332588324562778
1106	18-19	-0.2256131051943342
1106	20-21	-0.2434171395688054
1106	22-23	-0.1663568258647885
1106	24-25	-0.07729406346120982
1106	26-27	-0.1270484381237864
1106	28-29	-0.11519354183982955
1106	30-31	-0.17845615256968728
1106	32-33	-0.07528896246518002
1106	34-35	-0.12427175968056758
1106	36-37	-0.23630082789424023
1106	38-39	-0.21895790720162012
1106	40-41	-0.18783061249563815
1106	42-43	-0.2007636781322404
1106	44-45	-0.1509785411374125
1106	46-47	-0.20616604406772154
1106	48-49	-0.11551972849256487
1106	50-51	-0.03881808140572218
1106	52-53	-0.1810135160732287
1106	54-55	-0.17617302072921603
1106	56-57	-0.08793075112787818
1106	58-59	-0.18379890704375867
1106	60-61	-0.062397806675889456
1106	62-63	-0.15754823783712624
1106	64-65	-0.08665029467901064
1106	66-67	-0.016756591884060867
1106	68-69	0.045164858421138376
1106	70-71	0.011948239564574692
1106	72-73	-0.08300175963123024
1106	74-75	-0.09368061035652886
1106	76-77	-0.09380255908468982
1106	78-79	-0.013799907265557465
1106	80-81	0.08929689802323537
1106	82-83	-0.012589597218067183
1106	84-85	0.046369917938463345
1106	86-87	0.14554999978690475
1106	88-89	0.1071202363553958
1106	90-91	0.1350037655558758
1106	92-93	0.14239388192886793
1106	94-95	0.12311313873189533
1106	96-97	0.1642871348804249
1106	98-99	0.02854162666821125
1106	100	0.08844316873015501
1107	1	-0.03941405452650315
1107	2	-0.13240563962685314
1107	3	-0.15842543061971526
1107	4	-0.07804603369385177
1107	5	-0.11851870194298186
1107	6	-0.1847074334333101
1107	7	-0.24973099552151723
1107	8	-0.17620135566611594
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2316	92-93	0.21658336595128347
2316	94-95	0.17696520910851277
2316	96-97	0.21729238907338555
2316	98-99	0.16297594507119229
2316	100	-0.009798581791844896
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	9.0
12	48.0
13	285.0
14	1022.0
15	2886.0
16	6228.0
17	11893.0
18	19585.0
19	28097.0
20	36520.0
21	47482.0
22	59221.0
23	68616.0
24	74970.0
25	82490.0
26	94659.0
27	111586.0
28	133448.0
29	162299.0
30	198497.0
31	245214.0
32	306442.0
33	396815.0
34	564681.0
35	994542.0
36	1515658.0
37	1120028.0
38	1021366.0
39	189919.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.79776895527672	17.400031690521104	12.251429978031554	23.550769376170624
2	16.92592192757862	20.570303303901593	38.58514670121595	23.91862806730384
3	19.917324705278624	26.24813182681286	29.27918569864974	24.55535776925878
4	13.131143177183024	16.197779866690595	36.38296665797151	34.28811029815487
5	13.523209878487	36.21155209445108	33.1061649945047	17.159073032557224
6	30.478499179611617	34.561734342537136	19.503120971694234	15.456645506157015
7	28.498866053400164	31.501779743098417	21.73718991260532	18.262164290896095
8	27.49719265136466	32.707973297097546	20.406382395041987	19.38845165649581
9	27.93969168020638	16.008361196650238	20.357733900086476	35.694213223056906
10-11	25.88888429299951	24.942285265964117	27.898145603112635	21.27068483792374
12-13	26.442238411656394	23.052044138446348	27.746437434953165	22.759280014944096
14-15	23.751765220428616	24.411227886162507	26.44152579098389	25.395481102424988
16-17	22.960935744021388	26.72988025662335	27.096209606383255	23.212974392972004
18-19	22.63372195887769	26.28035211401035	28.68706583844567	22.398860088666297
20-21	23.591496487447543	25.346338339193146	28.47989539065054	22.58226978270877
22-23	23.158452545468688	25.27690443979781	28.365120641617985	23.199522373115514
24-25	23.096935262626964	25.353262755061195	28.360963294672736	23.18883868763911
26-27	22.984328013474904	25.021857747384562	28.833803517617678	23.160010721522855
28-29	22.8220264688128	25.910503900555824	28.546969642074455	22.72049998855692
30-31	23.666814676973345	25.225533345854718	28.52032951030829	22.58732246686365
32-33	23.324170741394667	25.484729002315635	28.519393063184655	22.671707193105046
34-35	23.593461120944976	25.524239903015033	28.389686606987375	22.492612369052615
36-37	23.510865749241717	25.39980387596496	28.423407212768957	22.665923162024367
38-39	23.384859796922235	25.932645625886007	28.08414212881518	22.598352448376573
40-41	23.371004709472444	25.701201797161428	28.1652232410834	22.76257025228273
42-43	23.059037705968915	25.852211315260078	27.749492533585123	23.339258445185887
44-45	23.089179453908685	25.68323463736203	27.878220318017288	23.349365590712
46-47	23.244493604739265	25.647774998813954	27.757865070737914	23.349866325708867
48-49	23.164258152793494	25.798517584739162	27.807673965529883	23.22955029693746
50-51	22.76660971796916	26.197170429987132	27.899706057175923	23.136513794867785
52-53	23.047436329507175	26.43136381490711	27.036490628784627	23.484709226801087
54-55	22.754810780214747	25.997317575842732	27.528292849784446	23.71957879415807
56-57	23.20294912152897	25.970245784812846	27.1804522891032	23.646352804554983
58-59	23.446208923181644	25.93523307575068	27.377337427985022	23.241220573082654
60-61	23.520751628405453	26.179099514734105	27.605775635510447	22.69437322134999
62-63	23.109442327516803	26.21541949992354	27.548596899053106	23.126541273506547
64-65	22.87178672918277	26.272180545932887	27.27481544217989	23.581217282704454
66-67	23.00252023338578	26.050264032074534	27.04207220612755	23.905143528412136
68-69	22.667688733844233	26.066582472882192	27.23430756616584	24.031421227107728
70-71	22.912387339830673	25.908695018384886	27.31133017973256	23.86758746205188
72-73	22.808487409663606	26.04313463412805	27.19704003719568	23.951337919012662
74-75	22.647893071073565	26.56526357744452	26.816482879071675	23.970360472410242
76-77	22.52636636857827	25.876139631519003	27.175682007554187	24.421811992348538
78-79	22.520295028897973	26.042805783450262	27.212101273960528	24.224797913691244
80-81	22.696620428152574	26.18801251778276	26.79605551610502	24.31931153795964
82-83	22.68156468560802	26.107892885146526	27.02780759387467	24.182734835370777
84-85	22.514699765197374	26.505583859295882	26.647386098710502	24.332330276796238
86-87	22.306534139087777	26.421688655191456	26.816133128545637	24.455644077175126
88-89	22.027015162638303	26.14689151104141	26.879318272368153	24.946775053952138
90-91	22.699896318565195	25.925522425071467	26.77987852973708	24.59470272662626
92-93	22.50832783992668	26.1942597130557	26.81935057025464	24.47806187676298
94-95	22.163930025257457	25.854878403750636	26.941905048268964	25.039286522722936
96-97	21.79638641188393	25.93140427167159	27.28355098212153	24.98865833432295
98-99	22.078115984117336	25.91030872750618	26.775559932492605	25.23601535588388
100	21.868713399534677	25.69115328333919	26.918288354125387	25.52184496300075
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3403.0
1	2577.0
2	5455.5
3	7866.0
4	4631.5
5	2970.5
6	4053.0
7	6850.0
8	10220.5
9	11647.0
10	10107.0
11	8263.0
12	8318.5
13	8937.5
14	9737.0
15	10557.0
16	11129.5
17	11769.5
18	12647.0
19	13481.0
20	14394.5
21	15656.5
22	17182.5
23	19392.5
24	22112.5
25	25532.0
26	30755.0
27	36861.5
28	42806.0
29	50448.5
30	60681.0
31	70273.5
32	81073.0
33	93709.0
34	106350.5
35	118551.0
36	134275.5
37	150017.0
38	160184.5
39	170176.0
40	177478.5
41	184061.0
42	193771.0
43	205700.0
44	217843.0
45	231447.0
46	247509.0
47	274790.0
48	322071.5
49	342808.5
50	341340.5
51	332453.0
52	307359.5
53	294883.5
54	290602.5
55	278664.0
56	257594.0
57	238023.5
58	219894.5
59	195274.0
60	163414.5
61	132499.5
62	108302.5
63	85994.5
64	64783.0
65	48295.5
66	36117.0
67	28209.5
68	21912.5
69	16323.0
70	12671.5
71	9972.0
72	7940.5
73	6280.5
74	4823.5
75	3511.5
76	2405.5
77	1510.5
78	961.5
79	603.0
80	372.0
81	245.5
82	178.0
83	118.5
84	75.5
85	58.5
86	37.0
87	25.5
88	20.0
89	11.0
90	10.0
91	8.0
92	4.5
93	3.0
94	1.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.045659413598099226
2	0.010460835844801225
3	0.0
4	3.4691547444493857E-4
5	4.2697289162453973E-4
6	0.0
7	0.0
8	6.671451431633434E-5
9	0.0
10-11	1.3342902863266867E-5
12-13	2.802009601286042E-4
14-15	0.011588311136747275
16-17	0.0018079633379726608
18-19	4.002870858980061E-5
20-21	9.473461032919476E-4
22-23	9.073173947021471E-4
24-25	0.0045499298763740014
26-27	0.017112272922139758
28-29	0.013469660440467905
30-31	0.010320735364736924
32-33	0.007505382860587613
34-35	0.009019802335568403
36-37	0.005123674699494478
38-39	0.004656673099280137
40-41	0.006264492894303795
42-43	0.007058395614668174
44-45	0.007512054312019247
46-47	0.016285012944617212
48-49	0.018366505791286843
50-51	0.017706032099555135
52-53	0.012041969834098349
54-55	0.0037426842531463568
56-57	0.004289743270540298
58-59	0.010414135684779792
60-61	0.0023083221953451682
62-63	9.340032004286808E-5
64-65	2.46843702970437E-4
66-67	0.001027403520471549
68-69	8.005741717960122E-5
70-71	1.8012918865410274E-4
72-73	0.002701937829811541
74-75	0.004963559865135275
76-77	0.004903516802250574
78-79	0.0031555965271626144
80-81	4.002870858980061E-5
82-83	9.006459432705136E-4
84-85	3.8027273160310575E-4
86-87	4.0028708589800603E-4
88-89	2.0014354294900302E-4
90-91	3.936156344663726E-4
92-93	4.0695853732963946E-4
94-95	3.335725715816717E-5
96-97	0.0017479202750879598
98-99	4.6033014878270697E-4
100	1.3342902863266868E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7494621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.03846742704054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.94834127892466	39.42016564057806
2	16.148835105504137	17.45316599666868
3	5.103449955185039	8.273478425063947
4	2.0315702312842	4.391317670759858
5	1.0510913861975162	2.8399683817938683
6	0.6178268255688166	2.0031848873431413
7	0.3922771445833231	1.4838638989956863
8	0.28189942768119014	1.218673043236109
9	0.21108913389437087	1.0266239957537842
>10	1.0986104633447715	10.668093021579798
>50	0.07148650332391161	2.676107717056489
>100	0.03955405999733524	3.8638281836675406
>500	0.0024071224796012526	0.8598967273706511
>1k	0.001203549898969416	1.300636795295191
>5k	1.9517025385530997E-4	0.6719127687206682
>10k+	1.626418782127583E-4	1.8490828461164672
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27864	0.371786645382068	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24629	0.3286223546193997	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	24211	0.32304502122255413	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15481	0.20656147922623438	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13009	0.1735778233482387	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	9839	0.13128082127168272	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2008612576940181E-4
2	0.0	0.0	0.0	1.3342902863266868E-5	1.2008612576940181E-4
3	0.0	0.0	0.0	1.3342902863266868E-5	1.2008612576940181E-4
4	0.0	0.0	0.0	1.3342902863266868E-5	1.2008612576940181E-4
5	0.0	0.0	0.0	1.3342902863266868E-5	1.2008612576940181E-4
6	0.0	0.0	0.0	1.3342902863266868E-5	1.3342902863266868E-4
7	0.0	0.0	0.0	1.3342902863266868E-5	1.3342902863266868E-4
8	0.0	0.0	0.0	1.3342902863266868E-5	1.3342902863266868E-4
9	0.0	0.0	0.0	1.3342902863266868E-5	1.7345773722246929E-4
10-11	0.0	0.0	0.0	2.6685805726533737E-5	1.7345773722246929E-4
12-13	0.0	0.0	0.0	4.00287085898006E-5	1.801291886541027E-4
14-15	0.0	0.0	0.0	6.0043062884700906E-5	2.2682934867553675E-4
16-17	0.0	0.0	0.0	6.671451431633434E-5	3.0021531442350454E-4
18-19	0.0	5.3371611453067474E-5	0.0	1.0674322290613495E-4	3.4691547444493857E-4
20-21	0.0	5.3371611453067474E-5	0.0	1.801291886541027E-4	3.4691547444493857E-4
22-23	0.0	5.3371611453067474E-5	0.0	4.336443430561732E-4	4.0695853732963946E-4
24-25	0.0	5.3371611453067474E-5	0.0	0.001307604480600153	4.5365869735107355E-4
26-27	0.0	5.3371611453067474E-5	0.0	0.0026352233154952063	4.5365869735107355E-4
28-29	0.0	5.3371611453067474E-5	0.0	0.006304521602893595	4.5365869735107355E-4
30-31	0.0	5.3371611453067474E-5	0.0	0.016611914064767253	4.6033014878270697E-4
32-33	0.0	5.3371611453067474E-5	0.0	0.04072921099012211	5.203732116674079E-4
34-35	0.0	5.3371611453067474E-5	0.0	0.07890992753336026	5.337161145306747E-4
36-37	0.0	5.3371611453067474E-5	0.0	0.13635112435972413	5.470590173939416E-4
38-39	0.0	6.671451431633434E-5	0.0	0.22382052407987008	5.870877259837422E-4
40-41	0.0	6.671451431633434E-5	0.0	0.33083727649470196	5.937591774153757E-4
42-43	0.0	8.00574171796012E-5	0.0	0.432556896472817	6.071020802786424E-4
44-45	0.0	8.00574171796012E-5	0.0	0.5536570828598271	6.271164345735428E-4
46-47	0.0	8.00574171796012E-5	0.0	0.6948383380560539	6.604736917317101E-4
48-49	0.0	9.340032004286808E-5	0.0	0.8405695231286545	7.338596574796778E-4
50-51	0.0	9.340032004286808E-5	0.0	0.9949268949023573	7.472025603429447E-4
52-53	0.0	9.340032004286808E-5	0.0	1.1581172683715426	7.939027203643787E-4
54-55	0.0	9.340032004286808E-5	0.0	1.3445643215314025	8.406028803858127E-4
56-57	6.671451431633434E-6	9.340032004286808E-5	0.0	1.55579581675978	8.539457832490796E-4
58-59	1.3342902863266868E-5	9.340032004286808E-5	0.0	1.78598757695686	8.739601375439799E-4
60-61	1.3342902863266868E-5	9.340032004286808E-5	0.0	2.0211829257276652	8.806315889756133E-4
62-63	1.3342902863266868E-5	9.340032004286808E-5	0.0	2.271829356014133	8.939744918388801E-4
64-65	1.3342902863266868E-5	9.340032004286808E-5	0.0	2.525904912336461	8.939744918388801E-4
66-67	1.3342902863266868E-5	9.340032004286808E-5	0.0	2.814772087874757	9.006459432705136E-4
68-69	1.3342902863266868E-5	9.340032004286808E-5	0.0	3.1487649608966217	9.073173947021471E-4
70-71	1.3342902863266868E-5	9.340032004286808E-5	0.0	3.5085496651531813	9.273317489970473E-4
72-73	1.3342902863266868E-5	9.340032004286808E-5	0.0	3.8437433994327397	0.0010941180347878831
74-75	1.3342902863266868E-5	9.340032004286808E-5	0.0	4.184821353874999	0.0010941180347878831
76-77	1.3342902863266868E-5	9.340032004286808E-5	0.0	4.556607999257068	0.0011007894862195165
78-79	1.3342902863266868E-5	9.340032004286808E-5	0.0	4.976996168318585	0.00110746093765115
80-81	2.0014354294900304E-5	9.340032004286808E-5	0.0	5.403755573497312	0.0011341467433776838
82-83	2.6685805726533737E-5	9.340032004286808E-5	0.0	5.841923160624133	0.0011741754519674844
84-85	2.6685805726533737E-5	9.340032004286808E-5	0.0	6.28849010510338	0.0011741754519674844
86-87	2.6685805726533737E-5	9.340032004286808E-5	0.0	6.756632523512529	0.0012008612576940181
88	2.6685805726533737E-5	9.340032004286808E-5	0.0	7.124483012549934	0.0012542328691470856
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	17660	0.0	59.45469	2
ACCTGGG	19240	0.0	53.613094	3
GTACCTG	19830	0.0	53.254993	1
CCTGGGG	15890	0.0	42.676018	4
TATAACG	925	0.0	36.583427	2
CTGGGGG	9185	0.0	33.000614	5
GTACCCG	1825	0.0	32.977703	1
ATAACGC	1085	0.0	30.751766	3
TAACGCA	1165	0.0	29.043438	4
TACCCGG	2160	0.0	26.763628	2
TGGGGGG	12065	0.0	25.239988	6
TACCGTA	690	0.0	25.199793	7
GTACCGT	1100	0.0	23.06973	6
GTATAAC	1980	0.0	22.797087	1
CCGTATG	955	0.0	21.658285	9
ACCGTAT	875	0.0	21.489662	8
GTACCGG	1325	0.0	21.291618	1
CATGGGG	19020	0.0	21.149723	4
GTACATG	39030	0.0	19.82917	1
ATGGGGG	11385	0.0	19.771696	5
>>END_MODULE