FastQCFastQC Report
Mon 6 Jun 2016
ERR522849_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522849_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6349932
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT588800.9272540241375813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206370.3249956062521614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174930.27548326501764114No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT166630.26241225890292996No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC135540.2134511046732469No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA98410.15497803756008727No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG94390.14864726110452836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94190.14833229710176424No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA86680.13650539879797138No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG237900.027.6057951
TACATGG244000.026.682762
ACATGGG251150.025.2313963
CATGGGG175550.023.0545124
TACCTGG45100.021.8883782
ATGGGGG99750.021.7249535
GAGTACT180350.020.75176412-13
GTACTTT192900.019.06026514-15
AGAGTAC289900.018.44299110-11
GTACCTG56350.018.2704181
GGACCGT8050.018.0929766
GAGTACA188050.016.9244141
AGTACTT190200.016.81136712-13
CATGGGA84600.016.6132454
AGTACAT181150.016.4521142
ACCTGGG60550.016.3038333
TATCACG4752.1827873E-1115.8342382
ACTTTTT235800.015.82077616-17
TATAACG5500.015.3844032
TGGGGGG91500.015.3016896
Produced by FastQC (version 0.11.5)