FastQCFastQC Report
Mon 6 Jun 2016
ERR522849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522849_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6349932
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT600910.946325094504949No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193400.30457019067290797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170430.26839657495544833No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT162560.25600274144668006No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC141490.22282128375547958No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC124770.19649029312439883No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA100740.1586473681922893No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG96600.15212761333507194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90570.14263144865173358No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC80730.12713521971573868No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG234750.026.8054391
TACATGG238550.025.574132
ACATGGG245350.024.268033
CATGGGG165850.022.5551174
ATGGGGG96800.020.8755115
GAGTACT180900.020.73696312-13
GTACTTT193750.019.35873614-15
TACCTGG45850.018.964352
AGAGTAC292500.018.26512510-11
GAGTACA187700.017.564111
AGTACTT194250.017.10957512-13
AGTACAT181050.016.588532
GTATAGG16050.016.4091241
GTACCTG56750.016.2428651
GTATAGT17250.016.085531
ACTTTTT236300.016.0794916-17
GTATTAC14450.015.9477951
CATGGGA86900.015.899194
TATAACG5155.456968E-1215.5148372
TGGGGGG85900.015.0447016
Produced by FastQC (version 0.11.5)