FastQCFastQC Report
Mon 6 Jun 2016
ERR522848_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522848_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5780712
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175100.302903863745504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138850.24019532542012126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87250.1509329646590247No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83500.051.284941
GTATCAA152800.040.251971
TACCTGG63700.038.8108862
TCAACGC184350.032.7815674
GTACCTG77350.032.426631
CAACGCA187500.032.3553125
ATCAACG186400.032.2465253
ACCTGGG75650.032.055243
TATCAAC191900.031.5952642
AACGCAG197050.030.9498586
GTACATG193300.030.7229121
TACATGG199250.029.5097982
ACATGGG203350.027.8484573
ACGCAGA223300.027.2484267
CATGGGG148800.026.4019034
CGCAGAG231850.026.2221768
ATGGGGG95800.024.280655
GCAGAGT256050.022.916749
TATAACG5200.022.5966422
CCTGGGG75150.021.8236984
Produced by FastQC (version 0.11.5)