FastQCFastQC Report
Mon 6 Jun 2016
ERR522848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522848_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5780712
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169060.2924553238424609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145560.2518028921004887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87940.15212658925059752No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87400.053.4608081
GTATCAA157450.041.0322761
TACCTGG67400.040.5463942
ACCTGGG79700.033.8444483
GTACCTG83450.033.0985871
TCAACGC196150.032.294734
ATCAACG198950.031.8641153
CAACGCA200850.031.6092115
TATCAAC205150.031.1361032
AACGCAG210400.030.1518786
GTACATG197000.027.8741951
TACATGG202100.026.415912
ACGCAGA240350.026.3553247
ACATGGG205750.025.238083
CGCAGAG251450.025.2177818
CCTGGGG81200.024.0171894
CATGGGG151250.023.6439154
TATAACG5200.023.5183032
GCAGAGT280400.022.2956529
ATGGGGG97550.022.2558825
Produced by FastQC (version 0.11.5)