FastQCFastQC Report
Mon 6 Jun 2016
ERR522847_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522847_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6637246
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239320.3605712369256767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197210.2971262478443619No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113970.17171278569454862No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78730.11861847519287368No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70690.10650501729181049No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG122550.053.4090922
GTACCTG140150.047.2541581
ACCTGGG138900.046.4452323
CCTGGGG125550.034.379184
TATAACG6750.032.043752
GGTATCA242100.025.412271
GTATCAA332950.025.1602731
CTGGGGG80400.024.9694255
ATAACGC8550.023.6478483
TAACGCA9500.022.7668954
GTACATG318600.021.8940371
CATGGGG175050.021.7835334
TCAACGC380000.021.7027914
CAACGCA383750.021.5380945
ATCAACG385550.021.3791523
TATCAAC388050.021.314122
TGGGGGG110900.021.3126246
AACGCAG394050.021.1187256
TACATGG326700.021.0852012
GTACCCG11950.020.861161
Produced by FastQC (version 0.11.5)