##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522847_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6637246
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.067413653192904	33.0	31.0	34.0	27.0	34.0
2	31.39930703186231	33.0	31.0	34.0	27.0	35.0
3	31.344796923302226	33.0	31.0	34.0	26.0	36.0
4	33.649682413458834	35.0	33.0	37.0	30.0	37.0
5	33.73067564468757	35.0	34.0	37.0	30.0	37.0
6	33.95337960955493	35.0	35.0	37.0	29.0	37.0
7	33.83638063136427	35.0	34.0	37.0	30.0	37.0
8	33.91740881684964	35.0	34.0	37.0	30.0	37.0
9	35.06151903967398	37.0	34.0	39.0	29.0	39.0
10-11	35.01934484573873	37.0	34.5	39.0	29.5	39.0
12-13	35.04227468742307	37.0	35.0	39.0	29.0	39.0
14-15	35.77439121587477	38.0	34.5	40.0	29.0	41.0
16-17	35.923714368881306	38.0	35.5	40.0	28.5	41.0
18-19	35.77994269008562	38.0	34.0	40.0	27.5	41.0
20-21	35.874620964779666	38.0	35.0	40.0	28.0	41.0
22-23	35.719325153836394	38.0	35.0	40.0	28.0	41.0
24-25	35.687826396671156	37.5	34.5	40.0	27.0	41.0
26-27	35.56987642163632	37.0	35.0	40.0	27.0	41.0
28-29	35.324978236455294	37.0	34.5	40.0	27.0	41.0
30-31	35.133650688854985	37.0	34.5	40.0	26.0	41.0
32-33	35.08664180896715	37.5	34.0	40.0	25.5	41.0
34-35	34.92336512764481	37.5	34.0	40.0	25.0	41.0
36-37	34.74263030178481	37.5	33.5	40.0	25.0	41.0
38-39	34.56886078955037	36.5	33.5	40.0	24.0	41.0
40-41	34.50657260556562	37.0	33.0	40.0	24.0	41.0
42-43	34.2982187943614	36.0	33.0	40.0	23.5	41.0
44-45	34.2004353914259	37.0	33.0	40.0	23.0	41.0
46-47	33.98029423649508	36.5	33.0	40.0	22.0	41.0
48-49	33.82901711040995	37.0	33.0	39.5	20.0	41.0
50-51	32.81888971721102	35.5	31.5	38.5	19.5	40.0
52-53	33.04601449155267	36.0	31.5	38.5	20.5	39.5
54-55	33.887833673785785	37.0	32.5	39.5	21.0	40.5
56-57	34.025841290197775	36.5	33.0	40.0	22.5	41.0
58-59	33.95632842296338	37.0	33.0	40.0	21.0	41.0
60-61	33.70639320887007	36.5	32.5	39.0	21.0	41.0
62-63	33.52094219500076	36.0	32.0	39.0	20.5	41.0
64-65	33.131800975886684	36.0	32.0	39.0	20.0	41.0
66-67	32.937073599501964	36.0	32.0	38.0	19.5	40.0
68-69	32.59521644971423	35.0	31.0	37.5	18.5	40.0
70-71	32.38275498602884	35.0	31.0	37.0	18.5	39.5
72-73	32.05878959435886	35.0	31.0	37.0	18.0	39.0
74-75	31.68712211359953	35.0	31.0	36.5	18.0	39.0
76-77	31.555361139243594	35.0	30.0	37.0	18.0	38.5
78-79	31.284538120178155	35.0	30.0	36.0	18.0	38.0
80-81	31.021399914964732	35.0	29.5	36.0	18.0	37.5
82-83	30.714182885491965	34.0	29.0	35.5	17.0	37.0
84-85	30.477361845560644	34.0	29.0	35.0	17.5	37.0
86-87	30.22655850031775	34.0	29.0	35.0	16.5	37.0
88-89	30.06405269595251	34.0	29.0	35.0	15.5	36.5
90-91	29.848648068792386	34.0	29.0	35.0	15.0	36.0
92-93	29.59423230659222	34.0	27.0	35.0	14.5	36.0
94-95	29.493482085792813	34.0	27.0	35.0	15.0	36.0
96-97	29.303414624077515	34.0	26.5	35.0	11.5	36.0
98-99	29.208273958807613	34.0	26.0	35.0	10.5	36.0
100	27.969765472004504	32.0	24.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10441120297614503
1101	2	0.13693093330318362
1101	3	0.15859476363154457
1101	4	0.16284936548876772
1101	5	0.06637388738751326
1101	6	0.24635465672844958
1101	7	0.16725390357829184
1101	8	0.27140727385307173
1101	9	0.2672762109481397
1101	10-11	0.1744660772149942
1101	12-13	0.19334371435021325
1101	14-15	0.11273750182753162
1101	16-17	0.18124109654768716
1101	18-19	0.20459568537393125
1101	20-21	0.19669967086426254
1101	22-23	0.19678387006068476
1101	24-25	0.12673900667786597
1101	26-27	0.16402337177693482
1101	28-29	0.13279532255111803
1101	30-31	0.08207166620697848
1101	32-33	0.05437995664777873
1101	34-35	0.0506883978107453
1101	36-37	0.12170720671696245
1101	38-39	-0.012141606979021446
1101	40-41	0.01584470540947791
1101	42-43	0.004395901877551012
1101	44-45	0.0017389910526190988
1101	46-47	-6.010255556105903E-4
1101	48-49	-0.033373159659994656
1101	50-51	-0.08908405099492
1101	52-53	-0.09607446970263567
1101	54-55	0.0659152849795035
1101	56-57	-0.031613746335864334
1101	58-59	0.048164429011002596
1101	60-61	0.10770360716974636
1101	62-63	-0.019156718392707717
1101	64-65	-0.029232421134217645
1101	66-67	-0.060930141437737007
1101	68-69	-0.03832404435286918
1101	70-71	-0.09656960154929806
1101	72-73	-0.07347857125802904
1101	74-75	-0.11617581030460045
1101	76-77	-0.08837681850210544
1101	78-79	-0.25710817976748856
1101	80-81	-0.208817597003506
1101	82-83	-0.33850164479462563
1101	84-85	-0.3984944514687392
1101	86-87	-0.41966801532155174
1101	88-89	-0.3935861838159198
1101	90-91	-0.41685040910412496
1101	92-93	-0.41784448462874124
1101	94-95	-0.36063335249282247
1101	96-97	-0.3572571229194246
1101	98-99	-0.26758591735503146
1101	100	-0.38631725858901333
1102	1	0.06367312463731878
1102	2	-0.024539648723514773
1102	3	0.06041629293655859
1102	4	-0.011178707963161116
1102	5	0.048041926153729264
1102	6	0.12758871557597473
1102	7	0.09095767806640964
1102	8	0.09866217211985173
1102	9	0.09261554172218922
1102	10-11	0.07337173419192311
1102	12-13	0.025817319686865403
1102	14-15	-0.08446097125681007
1102	16-17	0.0957678061848597
1102	18-19	0.04948147652834933
1102	20-21	0.008233001761325909
1102	22-23	-0.06960443479373168
1102	24-25	-0.09232589858673634
1102	26-27	0.009755423394516072
1102	28-29	-0.16024069152502562
1102	30-31	-0.10257620053950234
1102	32-33	-0.1888450228123304
1102	34-35	-0.2605355959469833
1102	36-37	-0.6705442100410792
1102	38-39	-0.3134417687180431
1102	40-41	-0.9583627359172837
1102	42-43	-1.0053466480607085
1102	44-45	-0.4376535898950138
1102	46-47	-0.3924953181283968
1102	48-49	-0.39594821562402416
1102	50-51	-0.39388891135210713
1102	52-53	-0.223504612357857
1102	54-55	-0.16547407798421432
1102	56-57	-0.0406949117556934
1102	58-59	-0.08316823412339147
1102	60-61	-0.09885975746198739
1102	62-63	-0.3812356697651609
1102	64-65	-0.1451945806940742
1102	66-67	-0.07667436906750424
1102	68-69	-0.11523671216457387
1102	70-71	-0.2750451009659187
1102	72-73	-0.22642658786486436
1102	74-75	-0.2820741466178127
1102	76-77	-0.32259035350948295
1102	78-79	-0.3151503741552979
1102	80-81	-0.32140409658801516
1102	82-83	-0.5026156842367939
1102	84-85	-0.655935774446597
1102	86-87	-0.5632252645581595
1102	88-89	-0.4729070811682732
1102	90-91	-0.37621084047679076
1102	92-93	-0.4143914998260989
1102	94-95	-0.6227875556003291
1102	96-97	-0.6977020705553123
1102	98-99	-0.6149504156201786
1102	100	-0.6125862728737523
1103	1	0.08476252706998366
1103	2	0.017738817487625624
1103	3	0.08974147572129709
1103	4	0.05039017245638888
1103	5	0.09518144366422376
1103	6	0.14664915356553365
1103	7	0.11466683560580293
1103	8	0.0418403268313412
1103	9	0.105803735837668
1103	10-11	0.0012050696186705068
1103	12-13	0.09523294233925839
1103	14-15	0.08780097345080407
1103	16-17	0.00548247717232897
1103	18-19	0.08578743940199018
1103	20-21	-0.014116073728288825
1103	22-23	0.038676570725691306
1103	24-25	-0.0031138916150865725
1103	26-27	-0.05427598196653349
1103	28-29	-0.1815422387954584
1103	30-31	-0.13573053222681608
1103	32-33	-0.17348525058747555
1103	34-35	-0.28309894706833205
1103	36-37	-0.309066714515005
1103	38-39	-0.2015805807488178
1103	40-41	-0.18731247280534546
1103	42-43	-0.182719320785111
1103	44-45	-0.19069202732473656
1103	46-47	-0.08002918653772895
1103	48-49	-0.23681839150648898
1103	50-51	-0.33821092859943036
1103	52-53	-0.11230367145905973
1103	54-55	-0.024002255603612355
1103	56-57	0.1067275079051484
1103	58-59	0.014591434148627513
1103	60-61	0.09790414966948902
1103	62-63	-0.014902917204011601
1103	64-65	-0.04748926635934936
1103	66-67	-0.006608966402765759
1103	68-69	-0.06574472335176296
1103	70-71	-0.03440257612012232
1103	72-73	-0.08019281479546692
1103	74-75	-0.031154727712991104
1103	76-77	-0.026530213980610284
1103	78-79	-0.1730878358757728
1103	80-81	-0.0321140876042918
1103	82-83	0.05355791371573204
1103	84-85	-0.04789139920436014
1103	86-87	-0.7753386637681672
1103	88-89	-0.27164069424120996
1103	90-91	-0.6115956499045581
1103	92-93	-0.21111117616073827
1103	94-95	-0.5662971796116416
1103	96-97	-0.47860041478634585
1103	98-99	-0.23927536513905423
1103	100	-0.2033353673236995
1104	1	0.05658671943347926
1104	2	-0.020198979448824872
1104	3	0.06274872188994607
1104	4	0.11067329911199408
1104	5	0.026744366422825294
1104	6	0.20688509691377277
1104	7	-0.06296730478950252
1104	8	0.14810678233894237
1104	9	0.08258993163455841
1104	10-11	-0.03697138484329088
1104	12-13	0.10266752104379151
1104	14-15	-0.0768045056347546
1104	16-17	0.06086009588180019
1104	18-19	0.12301861184996454
1104	20-21	-0.02371871463378028
1104	22-23	-0.03758576587786422
1104	24-25	0.1295908719694978
1104	26-27	0.16392014680946687
1104	28-29	0.009342355574602834
1104	30-31	0.08842840796381068
1104	32-33	0.02588142303100227
1104	34-35	-0.05766700316011253
1104	36-37	0.04529415538910797
1104	38-39	-0.0025490737901137095
1104	40-41	0.0890542942700776
1104	42-43	-0.1286160822722735
1104	44-45	-0.04301282363915959
1104	46-47	0.012313142217742268
1104	48-49	0.08029462062989268
1104	50-51	-0.16510172962117053
1104	52-53	-0.18060648813253977
1104	54-55	0.08646086902582084
1104	56-57	0.07881554137576074
1104	58-59	0.0014855463804508418
1104	60-61	-0.11134825272928595
1104	62-63	-0.02722771895273013
1104	64-65	-0.07871869099015072
1104	66-67	-0.0776379297239842
1104	68-69	-0.19341949740812225
1104	70-71	0.019958171696281113
1104	72-73	-0.011417315222153945
1104	74-75	0.011905019520451532
1104	76-77	-0.058591594508168754
1104	78-79	-0.16698094899927085
1104	80-81	-0.14483858774370972
1104	82-83	0.008286703298960418
1104	84-85	-0.04478344121172384
1104	86-87	-0.21202597857787353
1104	88-89	-0.14489782042876698
1104	90-91	-0.19612183996763477
1104	92-93	-0.2826166884924568
1104	94-95	-0.22453760420108182
1104	96-97	-0.24298728985050033
1104	98-99	-0.20137788108418775
1104	100	-0.06556307822614116
1105	1	0.050297579943183734
1105	2	0.1140923795408888
1105	3	0.10885030652086414
1105	4	0.07904410760932734
1105	5	0.05118140458201026
1105	6	0.11318573644923191
1105	7	0.054370482169510126
1105	8	-0.020475628848657834
1105	9	0.027295120725469246
1105	10-11	0.03721577137265086
1105	12-13	0.1515837579965975
1105	14-15	0.06382662511764181
1105	16-17	0.07315395327258756
1105	18-19	0.04594046560332998
1105	20-21	-0.00486957584259784
1105	22-23	-0.03179612764167672
1105	24-25	0.034664820653176776
1105	26-27	-0.010940050246638577
1105	28-29	-0.08868355987478083
1105	30-31	-0.10958448510218233
1105	32-33	-0.0152998830831379
1105	34-35	0.018320835147022763
1105	36-37	-0.02566054219573033
1105	38-39	-0.008981150381714542
1105	40-41	-0.07651718755164438
1105	42-43	-0.08404815463386939
1105	44-45	-0.12480483793157049
1105	46-47	-0.13955405327126869
1105	48-49	-0.11516942501813077
1105	50-51	-0.16338988932992748
1105	52-53	-0.017617995940462094
1105	54-55	0.05735435466858263
1105	56-57	0.08647390208754757
1105	58-59	0.031078104986640653
1105	60-61	0.08800050956789818
1105	62-63	0.044638975442957474
1105	64-65	0.10119157844325599
1105	66-67	0.16366363878642431
1105	68-69	0.0817026859233323
1105	70-71	0.09476949820882652
1105	72-73	0.06932256579560914
1105	74-75	0.14183583238538588
1105	76-77	0.1888025110632725
1105	78-79	0.14255167968281768
1105	80-81	0.16381956802184305
1105	82-83	0.18508336107160872
1105	84-85	0.10388893574953073
1105	86-87	0.18455561208103788
1105	88-89	0.16251015864096985
1105	90-91	-0.033113661966645225
1105	92-93	0.011185026557527067
1105	94-95	0.05752418701973383
1105	96-97	0.15481205518613095
1105	98-99	0.03823091703605641
1105	100	-0.015964996437393353
1106	1	0.08272328582831179
1106	2	0.0876103845724927
1106	3	0.06037895992167108
1106	4	0.1059721727082561
1106	5	0.13592089016664488
1106	6	0.12698723388983524
1106	7	0.1377864353204501
1106	8	-1.0542780572911639
1106	9	0.10527263418713773
1106	10-11	0.13819314397251503
1106	12-13	0.08255250648075219
1106	14-15	0.05434143748371412
1106	16-17	-0.0720733619317997
1106	18-19	0.08406855813839087
1106	20-21	0.08573474410873416
1106	22-23	0.036964012672143554
1106	24-25	0.09483316005565001
1106	26-27	0.06334985927237113
1106	28-29	0.02001620091179035
1106	30-31	-0.03390590097434654
1106	32-33	-0.03824636571089002
1106	34-35	0.0756318654087238
1106	36-37	0.10320899270544714
1106	38-39	-0.04991701977417051
1106	40-41	0.09292052224704861
1106	42-43	0.06660540357572842
1106	44-45	-0.04600469482380731
1106	46-47	0.10295042618520256
1106	48-49	0.020283197306220302
1106	50-51	0.018217443516896026
1106	52-53	0.18949398956274877
1106	54-55	0.13829531288575225
1106	56-57	0.05259493857877118
1106	58-59	0.08460749918165789
1106	60-61	0.15122144484643485
1106	62-63	0.1644069299753994
1106	64-65	0.12346147710616862
1106	66-67	0.22655362106150534
1106	68-69	0.19054215524106866
1106	70-71	0.14323036055237282
1106	72-73	0.13600174445063828
1106	74-75	0.22027783332002215
1106	76-77	0.04514259207907756
1106	78-79	0.06386798170784758
1106	80-81	0.08882474374938454
1106	82-83	0.20629332478629792
1106	84-85	0.11087155485731515
1106	86-87	0.0971608606112504
1106	88-89	0.14987503464181273
1106	90-91	0.11645809579709976
1106	92-93	0.0751998995691956
1106	94-95	0.11013107155979895
1106	96-97	0.08318355066641558
1106	98-99	0.1313430942380549
1106	100	0.09606265348035592
1107	1	-0.04491856518774284
1107	2	-0.10601876859114512
1107	3	-0.01917853157234717
1107	4	0.12659586012324553
1107	5	0.16634565732672968
1107	6	-0.019279117565396575
1107	7	0.09385371461387138
1107	8	0.14875114138010304
1107	9	0.1984278654168392
1107	10-11	0.13330712732103933
1107	12-13	0.0816577747918501
1107	14-15	0.10992338111050515
1107	16-17	0.18109116968327754
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2312	52-53	0.09021138775781168
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2312	56-57	-1.2084178903712797
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2312	78-79	0.42796557723816875
2312	80-81	0.33558343383525724
2312	82-83	0.3605638184026674
2312	84-85	0.39738265059505906
2312	86-87	0.4319635121749634
2312	88-89	0.48459134230570555
2312	90-91	0.5195034352832835
2312	92-93	0.5590771483824533
2312	94-95	0.6143005087302598
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2313	1	0.11859914468081101
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2313	64-65	0.19403426794350054
2313	66-67	0.1623838445982031
2313	68-69	0.18967723293999228
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2313	78-79	0.26433075914908244
2313	80-81	0.22564198368971233
2313	82-83	0.2821733588775359
2313	84-85	0.3385910499282865
2313	86-87	0.45303648387033846
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2313	90-91	0.37409412535883746
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2313	96-97	0.5677591411387581
2313	98-99	0.48442908430517306
2313	100	0.5892191230607757
2314	1	-0.35499721049759003
2314	2	-0.24897906765376732
2314	3	-0.2127578646978172
2314	4	-0.2146613286958612
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2314	78-79	0.18518064988804994
2314	80-81	0.17330951812248685
2314	82-83	0.18030179845868233
2314	84-85	0.2547513327527362
2314	86-87	0.2625861938162046
2314	88-89	0.3508847669269741
2314	90-91	0.34984918894233275
2314	92-93	0.38799465426505364
2314	94-95	0.34593841594951513
2314	96-97	0.4779728808028061
2314	98-99	0.5640611045686832
2314	100	0.5408202076154573
2315	1	-0.012096683307575518
2315	2	-0.1380726196343467
2315	3	-0.056741990560112754
2315	4	-0.05978103488173758
2315	5	0.011782546709305564
2315	6	-0.07264227285971714
2315	7	-0.0027574747600169758
2315	8	0.06624419384848323
2315	9	-0.002548131957809119
2315	10-11	-1.280635036946336
2315	12-13	-0.028884122899874853
2315	14-15	-0.03936748388406386
2315	16-17	-0.056426634934531705
2315	18-19	0.07880148265414988
2315	20-21	-0.04880435913291592
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2315	24-25	0.0928402010767968
2315	26-27	0.02036918635464957
2315	28-29	0.08564538349938289
2315	30-31	0.17135703794324542
2315	32-33	0.029513072342638225
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2315	80-81	-0.026943291992118645
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2315	84-85	0.09670624670153316
2315	86-87	0.12101846515124492
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2315	90-91	0.383739175697567
2315	92-93	0.3103374021210037
2315	94-95	0.3460287336862109
2315	96-97	0.35321704005696475
2315	98-99	0.31032572268712144
2315	100	0.35640674819219953
2316	1	-0.21821107335069811
2316	2	-0.5016314440522578
2316	3	-0.4399616626417391
2316	4	-0.4311262214598912
2316	5	-0.42685925797459134
2316	6	-0.3060778446777661
2316	7	-0.35518146143785856
2316	8	-0.37346294906260624
2316	9	-0.3395306145310073
2316	10-11	-0.4336326164525701
2316	12-13	-0.47727398476935434
2316	14-15	-0.4324940462542557
2316	16-17	-0.6033182734690925
2316	18-19	-0.39610925927816965
2316	20-21	-0.3132709159144582
2316	22-23	-0.4295582300856182
2316	24-25	-0.36907289526716625
2316	26-27	-0.3694763986043057
2316	28-29	-0.2995423558123065
2316	30-31	-0.30623239004989244
2316	32-33	-0.3452827810985397
2316	34-35	-0.40532286675720997
2316	36-37	-0.25932137062992666
2316	38-39	-0.36939195391989443
2316	40-41	-0.2703729474025778
2316	42-43	-0.21123025969364306
2316	44-45	-0.29049801049742996
2316	46-47	-1.7939024009472746
2316	48-49	-0.4605703201175473
2316	50-51	-0.2275471180107047
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2316	54-55	-0.35395889435959305
2316	56-57	-0.45923947745185245
2316	58-59	-0.5876702776489822
2316	60-61	-0.44386165627323493
2316	62-63	-0.41253014774743946
2316	64-65	-0.6437827576220911
2316	66-67	-0.6304613705934159
2316	68-69	-0.3807764768131534
2316	70-71	-0.38666916361983183
2316	72-73	-0.4518982848275961
2316	74-75	-0.45284246463599587
2316	76-77	-0.5203348218441413
2316	78-79	-0.5106198710443941
2316	80-81	-0.34531492463593594
2316	82-83	-0.33825693817849967
2316	84-85	-0.2561895191336312
2316	86-87	-0.248788660012071
2316	88-89	-0.1034370979233401
2316	90-91	-0.04828285230615137
2316	92-93	0.10937576562554696
2316	94-95	0.10017066675823827
2316	96-97	0.10017702638740289
2316	98-99	0.08335345452208998
2316	100	-0.19934957114682916
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	11938.0
1	1073.0
2	704.0
3	526.0
4	432.0
5	438.0
6	500.0
7	1213.0
8	2490.0
9	3691.0
10	6438.0
11	12210.0
12	19531.0
13	24982.0
14	28989.0
15	32602.0
16	36976.0
17	43409.0
18	51188.0
19	58988.0
20	67967.0
21	77925.0
22	86756.0
23	93650.0
24	97941.0
25	102633.0
26	110833.0
27	123079.0
28	139370.0
29	163739.0
30	194536.0
31	236578.0
32	297236.0
33	390059.0
34	552547.0
35	866456.0
36	880950.0
37	879469.0
38	776213.0
39	160845.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.722090773799415	17.33718942198052	12.449661943425825	23.491057860794236
2	16.586111915645102	19.898548808308842	39.498946785014326	24.01639249103173
3	19.259667444474456	26.063246216745238	29.31617954558473	25.360906793195575
4	13.192472766281968	16.204632762316194	36.54905666251651	34.053837808885326
5	13.715650344114326	36.062670717719854	33.62984733284758	16.591831605318237
6	30.754047229141047	34.75373121815438	19.350098934701588	15.14212261800299
7	28.125905873314366	31.7441574333991	21.882591114522327	18.247345578764207
8	27.077835828707613	33.27591683989046	20.65662958083845	18.98961775056348
9	27.71686772197096	15.736322279483868	20.26347709576946	36.283332902775705
10-11	25.396016622967526	25.275994078998593	28.101927255599534	21.226062042434346
12-13	25.888300604951787	23.26925467625812	27.956600083048887	22.8858446357412
14-15	23.60400384298373	24.56086873661457	26.21668309186827	25.61844432853343
16-17	22.948387897701892	27.05764561145651	26.82838736608857	23.165579124753034
18-19	22.71578724930482	26.2072599521888	28.67887219465304	22.398080603853337
20-21	23.55580596277148	25.677279560565548	28.5329162341289	22.233998242534074
22-23	23.290188572522506	25.499788360467623	28.269166215760556	22.940856851249315
24-25	22.92174934046384	25.573034104970883	28.351747885987805	23.153468668577474
26-27	22.818409597713227	25.538384441527313	28.43071903178891	23.212486928970552
28-29	22.8288501607006	25.93556478428901	28.445373124769464	22.790211930240925
30-31	23.544515867121	25.654357707894913	28.38243898254452	22.41868744243957
32-33	23.111933169483883	25.77291457295161	28.32856418808314	22.78658806948137
34-35	23.269970797400198	25.998529487165257	28.12953470735745	22.601965008077098
36-37	23.406872388335508	25.905763419728416	27.981660521336828	22.705703670599245
38-39	23.283825393266454	26.08422736129977	27.83488922128624	22.797058024147535
40-41	23.292447711158456	25.807294152906866	28.05639110011106	22.84386703582361
42-43	23.120867153822687	25.98053993964421	27.76166259360736	23.136930312925745
44-45	22.98532513881707	26.098522969452997	27.870343466536507	23.045808425193425
46-47	23.040406252359737	25.835497998942834	27.655433058974555	23.468662689722873
48-49	23.120894795603718	25.99261969434805	27.620949471247258	23.265536038800978
50-51	23.00124348026165	26.336061660068427	27.566833005214736	23.095861854455194
52-53	23.008974276379433	26.46192913068556	27.174249053974886	23.354847538960115
54-55	22.915116477655335	26.251257933298017	27.471790305247907	23.36183528379874
56-57	23.06055545237146	26.319019428982145	27.17691105989924	23.44351405874716
58-59	23.020929277915176	26.28653258122222	27.436217269326097	23.256320871536506
60-61	23.325738574673327	26.284960822251684	27.497567296000607	22.89173330707438
62-63	23.08611114699066	26.348597098258374	27.50593542179508	23.05935633295588
64-65	23.016081922250482	26.09737342173465	27.41471473868925	23.47182991732562
66-67	22.959755498629583	26.147641072689183	27.28033769251605	23.612265736165185
68-69	22.783609365394923	26.167928572842204	27.388019644009358	23.660442417753515
70-71	22.76970058934703	26.02387382435638	27.459869362227618	23.746556224068975
72-73	22.76376673189682	26.346710237053767	27.157255141171937	23.732267889877477
74-75	22.85239593907019	26.33613802526028	27.115917470880557	23.695548564788975
76-77	22.788506122577246	26.055540052725068	27.247527256569594	23.908426568128093
78-79	22.672829301143803	26.079652692715467	27.360676907759533	23.886841098381197
80-81	22.721354928853863	26.295131143624552	27.02100746684919	23.962506460672397
82-83	22.874848072589764	26.26173589671731	27.021633556751922	23.841782473941002
84-85	22.870221060818448	26.381901138903462	26.831229925839633	23.91664787443845
86-87	22.668996735747868	26.287336980021376	27.077308356249773	23.966357927980983
88-89	22.53361956595808	26.037778354957165	27.247649089439086	24.180952989645675
90-91	23.040916959055846	25.99595512564538	27.118365454972064	23.844762460326706
92-93	22.76847990852095	26.04600085044097	27.138817494310878	24.0467017467272
94-95	22.661114085863854	25.823028635386237	27.186423121040743	24.32943415770917
96-97	22.66270109025904	25.786706762591493	27.219377125198335	24.331215021951127
98-99	22.721640426899047	25.765294349442115	27.049425188469883	24.463640035188952
100	22.734087993904048	25.690104640819516	27.166390431906862	24.409416933369577
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5846.0
1	6266.5
2	7266.0
3	5937.5
4	3096.5
5	2359.0
6	3044.0
7	4872.0
8	7595.5
9	8999.0
10	7998.0
11	6868.5
12	6988.0
13	7396.0
14	7874.0
15	8408.5
16	8867.0
17	9437.5
18	10112.5
19	10739.5
20	11676.5
21	12875.5
22	14337.0
23	16763.0
24	19613.0
25	23057.5
26	27902.0
27	33598.0
28	40004.5
29	48059.5
30	58046.0
31	67315.5
32	77022.0
33	88112.5
34	99699.5
35	111013.5
36	124911.0
37	139385.5
38	150576.5
39	161358.5
40	169771.5
41	176969.0
42	184966.0
43	194934.5
44	205912.0
45	217385.5
46	230351.5
47	243768.0
48	259540.0
49	275327.0
50	290472.0
51	288149.0
52	270669.5
53	260803.5
54	252711.0
55	238734.0
56	219407.5
57	199510.5
58	181118.0
59	159756.5
60	134965.0
61	111647.5
62	91710.5
63	72570.5
64	55001.0
65	42096.0
66	32183.5
67	25202.0
68	19995.0
69	15536.5
70	12230.5
71	9659.5
72	7793.5
73	6192.0
74	4823.0
75	3866.5
76	2904.5
77	1935.5
78	1253.5
79	825.0
80	610.0
81	413.5
82	292.5
83	223.0
84	151.0
85	102.5
86	83.0
87	73.5
88	60.5
89	40.0
90	34.5
91	32.0
92	22.0
93	17.5
94	16.5
95	12.5
96	11.5
97	13.0
98	8.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14772693373124937
2	0.07076730318568876
3	0.31318712610621935
4	0.31085182016758156
5	0.31180100903296337
6	0.2594901560074766
7	0.25994215070527743
8	0.25909842726938254
9	0.27003669895616345
10-11	0.24718836698232974
12-13	0.25686858676023155
14-15	0.23063782779785472
16-17	0.23943665791504487
18-19	0.2535765587112486
20-21	0.25490240982479784
22-23	0.2458625158687805
24-25	0.2456591182547701
26-27	0.2442353349566974
28-29	0.2540210201640861
30-31	0.24022011539123306
32-33	0.25217537514806593
34-35	0.23724448363071068
36-37	0.24451406502034126
38-39	0.2459077153385606
40-41	0.26333211093878395
42-43	0.24868748273003594
44-45	0.23476604603776927
46-47	0.23723695038574735
48-49	0.24681923797912567
50-51	0.23971538797868874
52-53	0.25683092053541484
54-55	0.26754319487329536
56-57	0.2632191122643337
58-59	0.25934702435317303
60-61	0.24125970319617504
62-63	0.26933610717457207
64-65	0.2615467318824705
66-67	0.2671740658700913
68-69	0.2716036139085398
70-71	0.29121265054813394
72-73	0.29993614821569065
74-75	0.27896359423773054
76-77	0.2769672843224434
78-79	0.276070828171805
80-81	0.26193846062056464
82-83	0.2731705288609161
84-85	0.28352874068551925
86-87	0.27529490394058015
88-89	0.2840485345879903
90-91	0.27871499715394005
92-93	0.25840536873275455
94-95	0.2799655158178558
96-97	0.2780068721273854
98-99	0.26448469741817615
100	0.28751382727113023
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6637246.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.505115639467085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4204959315145	44.87905331990727
2	14.997352302000492	17.84838366032734
3	4.608998665432625	8.227769957061536
4	1.8674302306584833	4.44486607295879
5	0.8882019648122883	2.6426280313678543
6	0.5194370460359502	1.8545496895075446
7	0.3455419469691424	1.4393059458879796
8	0.23522481646979385	1.1197663924246004
9	0.17178933037770658	0.9200109572776841
>10	0.8652782807192326	9.09662257349599
>50	0.051957317662505394	2.1120162437991503
>100	0.0263546586552796	2.7650137466618463
>500	0.001033339873540369	0.4267141436589293
>1k	7.427101008851303E-4	0.87838623509907
>5k	6.458348703176651E-5	0.2871141059362714
>10k+	9.687523054764977E-5	1.0577989246280108
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23932	0.3605712369256767	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19721	0.2971262478443619	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11397	0.17171278569454862	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7873	0.11861847519287368	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	7069	0.10650501729181049	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.51994697800865E-5	0.0
5	0.0	0.0	0.0	4.51994697800865E-5	0.0
6	3.0132979853391E-5	0.0	0.0	4.51994697800865E-5	1.50664899266955E-5
7	3.0132979853391E-5	0.0	0.0	4.51994697800865E-5	1.50664899266955E-5
8	3.0132979853391E-5	0.0	0.0	4.51994697800865E-5	1.50664899266955E-5
9	3.0132979853391E-5	0.0	0.0	4.51994697800865E-5	1.50664899266955E-5
10-11	4.51994697800865E-5	0.0	0.0	4.51994697800865E-5	1.50664899266955E-5
12-13	4.51994697800865E-5	0.0	0.0	6.0265959706782E-5	1.50664899266955E-5
14-15	4.51994697800865E-5	0.0	0.0	6.779920467012975E-5	1.50664899266955E-5
16-17	4.51994697800865E-5	0.0	0.0	7.53324496334775E-5	9.0398939560173E-5
18-19	4.51994697800865E-5	0.0	0.0	9.793218452352074E-5	1.054654294868685E-4
20-21	4.51994697800865E-5	0.0	0.0	1.5819814423030274E-4	1.1299867445021625E-4
22-23	4.51994697800865E-5	0.0	0.0	3.3899602335064877E-4	1.355984093402595E-4
24-25	4.51994697800865E-5	0.0	0.0	8.964561506383822E-4	1.355984093402595E-4
26-27	4.51994697800865E-5	0.0	0.0	0.0018230452811301557	1.50664899266955E-4
28-29	6.0265959706782E-5	0.0	0.0	0.004791143796689169	1.50664899266955E-4
30-31	6.779920467012975E-5	0.0	0.0	0.013770771792999688	1.50664899266955E-4
32-33	7.53324496334775E-5	1.50664899266955E-5	0.0	0.036415706152823024	1.50664899266955E-4
34-35	7.53324496334775E-5	1.50664899266955E-5	0.0	0.07279374608082931	1.50664899266955E-4
36-37	7.53324496334775E-5	1.50664899266955E-5	0.0	0.1267317800183992	1.50664899266955E-4
38-39	7.53324496334775E-5	2.259973489004325E-5	0.0	0.208309289726492	1.657313891936505E-4
40-41	7.53324496334775E-5	3.7666224816738754E-5	0.0	0.30768785728297554	1.80797879120346E-4
42-43	7.53324496334775E-5	4.51994697800865E-5	0.0	0.4030210722941413	1.9586436904704148E-4
44-45	7.53324496334775E-5	4.51994697800865E-5	0.0	0.5188221138707229	2.1846410393708474E-4
46-47	7.53324496334775E-5	4.51994697800865E-5	0.0	0.6558970392237986	2.259973489004325E-4
48-49	7.53324496334775E-5	4.51994697800865E-5	0.0	0.7990964927320758	2.41063838827128E-4
50-51	7.53324496334775E-5	4.51994697800865E-5	0.0	0.9504167843108422	2.41063838827128E-4
52-53	7.53324496334775E-5	4.51994697800865E-5	0.0	1.1052701677774186	2.561303287538235E-4
54-55	7.53324496334775E-5	4.51994697800865E-5	0.0	1.2865275748405287	2.561303287538235E-4
56-57	7.53324496334775E-5	4.51994697800865E-5	0.0	1.489405394948447	2.71196818680519E-4
58-59	7.53324496334775E-5	4.51994697800865E-5	0.0	1.7123216466588702	2.862633086072145E-4
60-61	7.53324496334775E-5	4.51994697800865E-5	0.0	1.9427108773729347	2.862633086072145E-4
62-63	9.0398939560173E-5	4.51994697800865E-5	0.0	2.1838500486496963	3.0132979853391E-4
64-65	9.0398939560173E-5	4.51994697800865E-5	0.0	2.43156574277946	3.088630434972577E-4
66-67	9.0398939560173E-5	6.0265959706782E-5	0.0	2.7173318572190936	3.163962884606055E-4
68-69	9.0398939560173E-5	6.0265959706782E-5	0.0	3.0424968428170356	3.163962884606055E-4
70-71	9.0398939560173E-5	6.0265959706782E-5	0.0	3.3949020422024434	3.465292683139965E-4
72-73	9.0398939560173E-5	6.0265959706782E-5	0.0	3.7251745678855355	4.82127677654256E-4
74-75	9.0398939560173E-5	6.0265959706782E-5	0.0	4.0607429647778615	4.82127677654256E-4
76-77	9.0398939560173E-5	6.0265959706782E-5	0.0	4.428478317663682	4.82127677654256E-4
78-79	1.054654294868685E-4	6.0265959706782E-5	0.0	4.847145939746696	4.82127677654256E-4
80-81	1.054654294868685E-4	6.0265959706782E-5	0.0	5.270777970260557	4.82127677654256E-4
82-83	1.054654294868685E-4	6.0265959706782E-5	0.0	5.708150639587563	4.971941675809515E-4
84-85	1.054654294868685E-4	6.0265959706782E-5	0.0	6.155610323920493	4.971941675809515E-4
86-87	1.054654294868685E-4	6.0265959706782E-5	0.0	6.617066777395324	4.971941675809515E-4
88	1.20531919413564E-4	6.0265959706782E-5	0.0	6.988380421638734	4.971941675809515E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	12255	0.0	53.409092	2
GTACCTG	14015	0.0	47.254158	1
ACCTGGG	13890	0.0	46.445232	3
CCTGGGG	12555	0.0	34.37918	4
TATAACG	675	0.0	32.04375	2
GGTATCA	24210	0.0	25.41227	1
GTATCAA	33295	0.0	25.160273	1
CTGGGGG	8040	0.0	24.969425	5
ATAACGC	855	0.0	23.647848	3
TAACGCA	950	0.0	22.766895	4
GTACATG	31860	0.0	21.894037	1
CATGGGG	17505	0.0	21.783533	4
TCAACGC	38000	0.0	21.702791	4
CAACGCA	38375	0.0	21.538094	5
ATCAACG	38555	0.0	21.379152	3
TATCAAC	38805	0.0	21.31412	2
TGGGGGG	11090	0.0	21.312624	6
AACGCAG	39405	0.0	21.118725	6
TACATGG	32670	0.0	21.085201	2
GTACCCG	1195	0.0	20.86116	1
>>END_MODULE