FastQCFastQC Report
Mon 6 Jun 2016
ERR522847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522847_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6637246
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222410.3350938024596346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199490.3005614075476485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116220.17510274592805508No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73410.11060310255187167No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72040.10853899343191437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG125150.053.700762
GTACCTG145700.046.4067041
ACCTGGG142450.046.384053
CCTGGGG125000.035.6405264
TATAACG7350.031.9711232
ATAACGC8850.028.1435173
TAACGCA9400.026.9967634
CTGGGGG72900.026.5590295
GGTATCA245750.026.0403021
GTATCAA330850.024.0890671
TATCACG7050.023.9987492
GTACCCG13100.023.330471
TGGGGGG95700.021.6553846
CATGGGG165300.021.4928864
GTACCGG10600.020.8484331
TCAACGC381900.020.5254274
GAGTACT214550.020.41939212-13
CAACGCA386050.020.3898375
TATCAAC385850.020.3532222
ATCAACG385650.020.2527263
Produced by FastQC (version 0.11.5)