FastQCFastQC Report
Mon 6 Jun 2016
ERR522846_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522846_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9592954
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219500.22881377310888804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171170.17843304575420668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101070.10535857880690348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144300.046.303181
GTATCAA249250.038.5297851
TCAACGC292250.032.2083054
CAACGCA294700.031.876095
ATCAACG301450.031.2269693
AACGCAG307700.030.7520146
TATCAAC316000.029.9424062
GTACATG332150.027.3691731
ACGCAGA351200.026.8226387
TACATGG340950.026.2623922
CGCAGAG363450.025.891658
ACATGGG344700.025.1819733
CATGGGG228150.022.5957934
GCAGAGT411750.022.5227349
GAGTACT219050.020.47298212-13
ATGGGGG132500.019.541965
AGAGTAC362650.019.5352510-11
CAGAGTA400150.019.37751210-11
GTACTTT233150.018.95223414-15
GAGTACA256850.017.897971
Produced by FastQC (version 0.11.5)