FastQCFastQC Report
Mon 6 Jun 2016
ERR522846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522846_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9592954
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199190.20764198389776495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166530.17359616234999145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96700.10080315198008871No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141800.045.553561
GTATCAA243300.038.3643231
TCAACGC289100.031.502954
CAACGCA295000.030.8728925
ATCAACG296300.030.7701233
AACGCAG308250.029.6671816
TATCAAC312450.029.4891192
GTACATG327100.027.1837441
TACATGG336200.025.7411522
ACGCAGA357000.025.5499067
ACATGGG338800.024.6908343
CGCAGAG373350.024.423648
CATGGGG224700.021.85544
GCAGAGT420950.021.4271649
GAGTACT221900.019.77326612-13
AGAGTAC368000.019.35018210-11
GTACTTT233150.018.51488314-15
CAGAGTA409350.018.38301710-11
ATGGGGG127450.018.1782745
AGTACTT244000.017.40442712-13
Produced by FastQC (version 0.11.5)