FastQCFastQC Report
Mon 6 Jun 2016
ERR522845_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522845_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7399099
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228780.3091998093281358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181010.24463789442471304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117350.15860039175040094No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119600.045.436311
GTATCAA207500.039.045171
TCAACGC243700.032.6286854
ATCAACG247600.032.0972823
CAACGCA248500.031.9030085
TATCAAC255500.031.2926032
AACGCAG257050.031.0549226
TACCTGG66800.029.2011472
GTACATG218100.028.6480241
TACATGG223500.027.276632
ACGCAGA292900.027.1736977
CGCAGAG302650.026.3284428
ACATGGG226250.025.8824143
GTACCTG82100.024.2982461
CATGGGG165050.024.2593294
GCAGAGT340650.023.2116979
ACCTGGG84150.022.6191483
ATGGGGG101400.021.7822555
GAGTACT218250.020.35381512-13
AGAGTAC300050.019.76781310-11
Produced by FastQC (version 0.11.5)