FastQCFastQC Report
Mon 6 Jun 2016
ERR522845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7399099
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206340.2788717923628269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180540.24400268194816693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114980.1553972990495194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123800.047.764331
GTATCAA201750.035.8617821
TACCTGG67050.030.3011762
TCAACGC249700.028.8141124
ATCAACG252400.028.3947413
CAACGCA256050.028.0995275
TATCAAC260350.027.7103672
AACGCAG268050.027.1392526
GTACCTG81600.026.0577771
GTACATG216250.026.0174141
TACATGG220350.025.1424032
ACGCAGA304100.023.9063747
ACATGGG224600.023.6233563
CGCAGAG316000.022.9655238
ACCTGGG85400.022.892483
CATGGGG160900.022.0223644
GCAGAGT352250.020.4018559
ATGGGGG96300.020.2521175
GAGTACT221100.019.71728712-13
AGAGTAC311350.019.17996410-11
Produced by FastQC (version 0.11.5)