##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522845_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7399099
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44959217331732	34.0	31.0	34.0	30.0	35.0
2	32.55696254368269	34.0	31.0	34.0	30.0	34.0
3	32.789621817467236	34.0	31.0	34.0	31.0	36.0
4	35.6001821302837	37.0	35.0	37.0	32.0	37.0
5	35.69984358906402	37.0	35.0	37.0	35.0	37.0
6	35.783624871082274	37.0	35.0	37.0	35.0	37.0
7	35.61867140850528	37.0	35.0	37.0	35.0	37.0
8	35.65222657515462	37.0	35.0	37.0	33.0	37.0
9	36.85612788800366	38.0	35.0	39.0	34.0	39.0
10-11	36.94759260553211	38.0	36.0	39.0	33.5	39.0
12-13	36.8167125483792	38.0	35.5	39.0	33.0	39.0
14-15	37.86741304583166	39.0	36.0	41.0	33.5	41.0
16-17	37.88980638047957	39.0	37.0	41.0	33.5	41.0
18-19	37.888256326885205	39.0	37.0	41.0	33.5	41.0
20-21	37.76767542912995	39.0	36.5	41.0	33.5	41.0
22-23	37.67226509606102	39.0	36.5	41.0	33.5	41.0
24-25	37.51866416438001	38.0	36.0	41.0	33.0	41.0
26-27	37.377200386155124	38.0	36.5	41.0	33.0	41.0
28-29	37.33562782441484	38.0	36.0	40.5	33.5	41.0
30-31	37.16709162831853	38.0	36.0	40.0	33.0	41.0
32-33	36.98678278260637	38.0	36.0	40.0	32.0	41.0
34-35	36.79323482494287	38.0	36.0	40.0	31.5	41.0
36-37	36.79945321720929	38.0	35.5	40.0	32.0	41.0
38-39	36.910087430915574	38.0	36.0	40.0	32.0	41.0
40-41	36.82870049447912	38.0	36.5	40.0	31.5	41.0
42-43	36.85298777594407	38.0	36.5	40.0	32.5	41.0
44-45	36.66445245563007	38.0	35.5	40.0	31.0	41.0
46-47	36.41868624814994	38.0	35.5	40.0	30.5	41.0
48-49	36.35862008333717	37.5	35.5	40.0	30.5	41.0
50-51	35.97190583610248	37.0	35.0	40.0	29.5	41.0
52-53	35.993243771978186	37.5	35.0	40.0	29.5	41.0
54-55	35.80270942448533	37.0	35.0	40.0	29.0	41.0
56-57	35.48948446290555	37.0	35.0	40.0	27.5	41.0
58-59	35.36819760081599	36.5	35.0	40.0	28.0	41.0
60-61	35.089684230471846	36.5	34.5	39.5	28.0	41.0
62-63	34.873527303797395	36.5	34.0	39.0	26.5	41.0
64-65	34.73699000378289	37.0	34.0	39.0	26.0	41.0
66-67	34.49577022823995	36.5	34.0	39.0	26.0	40.0
68-69	34.03843474725774	36.0	33.5	38.0	25.5	40.0
70-71	33.768179274260284	36.0	33.0	37.5	25.5	40.0
72-73	33.51908441825147	35.5	33.0	37.0	25.0	39.0
74-75	33.21025228882598	35.0	32.5	37.0	24.5	39.0
76-77	31.774992671404988	34.0	30.5	36.0	22.5	37.5
78-79	32.82635487104579	35.0	32.0	37.0	25.5	37.0
80-81	32.851308179549974	35.0	33.0	36.5	25.0	37.0
82-83	32.63893840317584	35.0	32.5	36.0	24.5	37.5
84-85	32.41784114525296	35.0	32.0	36.0	24.5	37.0
86-87	32.160871749384626	35.0	32.0	36.0	24.5	37.0
88-89	31.989290655524407	35.0	32.0	36.0	23.0	36.5
90-91	31.823544461291842	35.0	31.5	35.5	23.0	36.5
92-93	31.651704890014308	34.5	31.0	35.0	23.0	36.5
94-95	31.5222808885244	35.0	31.0	35.0	21.0	36.0
96-97	31.31021716292754	34.5	31.0	35.0	21.5	36.5
98-99	31.14951266363648	34.0	31.0	35.0	20.0	36.5
100	29.92696394520468	34.0	29.0	35.0	18.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.17010061443357927
1101	2	-0.1858935528914074
1101	3	-0.17605391356892142
1101	4	-0.18552539458960382
1101	5	-0.20205107841016456
1101	6	-0.26500399941945574
1101	7	-0.22392399072436575
1101	8	-0.21968269852112599
1101	9	-0.32457211515168893
1101	10-11	-0.17278065272910936
1101	12-13	-0.227636842758848
1101	14-15	-0.3275116877181006
1101	16-17	-0.23211299792912854
1101	18-19	-0.27589145742111754
1101	20-21	-0.26440687243849226
1101	22-23	-0.227143113314213
1101	24-25	-0.37507033849907145
1101	26-27	-0.33319615499980415
1101	28-29	-0.4032966091572874
1101	30-31	-0.3973411956114319
1101	32-33	-0.31276951389565966
1101	34-35	-0.3100225629213753
1101	36-37	-0.3991640312059559
1101	38-39	-0.3031360410964865
1101	40-41	-0.38151409316644447
1101	42-43	-0.42354780844578954
1101	44-45	-0.4885061723043833
1101	46-47	-0.44890120733223426
1101	48-49	-0.5196983152542387
1101	50-51	-0.39521532140304316
1101	52-53	-0.6219771626962185
1101	54-55	-0.5598118967044243
1101	56-57	-0.6543887125918673
1101	58-59	-0.7041981891105991
1101	60-61	-0.725965136253869
1101	62-63	-0.6087588608785808
1101	64-65	-0.6222657763206882
1101	66-67	-0.7099691290321672
1101	68-69	-0.695888124165343
1101	70-71	-0.766201265279669
1101	72-73	-0.813079153200178
1101	74-75	-0.8393013313819466
1101	76-77	-0.7080959797458632
1101	78-79	-0.661485046296157
1101	80-81	-0.3915548265218902
1101	82-83	-0.5491570146715432
1101	84-85	-0.648826288779027
1101	86-87	-0.5718856777647616
1101	88-89	-0.6694626341841854
1101	90-91	-0.7117951290766378
1101	92-93	-0.9955660119268082
1101	94-95	-0.9474325786437738
1101	96-97	-0.690533563368767
1101	98-99	-0.7562108490064468
1101	100	-0.9976629235091501
1102	1	-0.018287241252807007
1102	2	-0.07273222017497716
1102	3	-0.11562289741494425
1102	4	-0.0572676444456377
1102	5	-0.09746189864596744
1102	6	-0.09461982171499983
1102	7	-0.1587521175321669
1102	8	-0.1264258224029433
1102	9	-0.1507458552055354
1102	10-11	-0.1327599632645402
1102	12-13	-0.14545538686999038
1102	14-15	-0.24602786712586067
1102	16-17	-0.20057050813177568
1102	18-19	-0.18846752189298854
1102	20-21	-0.20968421380162283
1102	22-23	-0.14635546233538577
1102	24-25	-0.2436827863730997
1102	26-27	-0.27303138468791843
1102	28-29	-0.17364699239456627
1102	30-31	-0.11793163382917982
1102	32-33	-0.223122944557538
1102	34-35	-0.27930402638392593
1102	36-37	-0.28191287400419185
1102	38-39	-0.29158735474945985
1102	40-41	-0.29574270050297
1102	42-43	-0.2958432191104876
1102	44-45	-0.32965372089835654
1102	46-47	-0.24431917053031071
1102	48-49	-0.28675740214915635
1102	50-51	-0.25813616242523096
1102	52-53	-0.40122573862046096
1102	54-55	-0.39039195908150504
1102	56-57	-0.2826384762579295
1102	58-59	-0.470148202493192
1102	60-61	-0.39105152721176495
1102	62-63	-0.2730903950708097
1102	64-65	-0.30982994482902626
1102	66-67	-0.3466568608568892
1102	68-69	-0.37924841410499965
1102	70-71	-0.32125032259409636
1102	72-73	-0.4317434084889271
1102	74-75	-0.7841608034703711
1102	76-77	-0.37191177291917654
1102	78-79	-0.3925808437097871
1102	80-81	-0.35516639917365467
1102	82-83	-0.24692251780110297
1102	84-85	-0.3093489310720443
1102	86-87	-0.3910966149441393
1102	88-89	-0.3221051952077545
1102	90-91	-0.3453944569920928
1102	92-93	-0.41183105148170895
1102	94-95	-0.41746970649334614
1102	96-97	-0.5902198413553918
1102	98-99	-0.8150682897177646
1102	100	-0.5617825558033331
1103	1	-0.0959008481522261
1103	2	-0.08140409614264144
1103	3	-0.1262636264094823
1103	4	-0.03368731280602333
1103	5	-0.11366008806430727
1103	6	-0.12377622219848661
1103	7	-0.03365939095307624
1103	8	-0.0489302645028431
1103	9	-0.13762122498918217
1103	10-11	-0.10079647967673111
1103	12-13	-0.1464045630482289
1103	14-15	-0.17616927526903936
1103	16-17	-0.20930173145811892
1103	18-19	-0.19586472108509412
1103	20-21	-0.16647769303553162
1103	22-23	-0.09451530972051359
1103	24-25	-0.18194782651122665
1103	26-27	-0.24317625857595004
1103	28-29	-0.2689362796562662
1103	30-31	-0.2295690638502208
1103	32-33	-0.18222245904213707
1103	34-35	-0.08809831288279923
1103	36-37	-0.12944137937412847
1103	38-39	-0.19577480106314482
1103	40-41	-0.34661900152944725
1103	42-43	-0.3435426460474602
1103	44-45	-0.4154468144037864
1103	46-47	-0.3643128964989515
1103	48-49	-0.3546070477585417
1103	50-51	-0.17592031754924164
1103	52-53	-0.30003731803564904
1103	54-55	-0.27909884353361036
1103	56-57	-0.11043953217129854
1103	58-59	-0.2665211337813318
1103	60-61	-0.2884808297441097
1103	62-63	-0.32330145975480207
1103	64-65	-0.3607272316920316
1103	66-67	-0.340022049222533
1103	68-69	-0.2006322360150321
1103	70-71	-0.40148894945941294
1103	72-73	-0.4269360963307989
1103	74-75	-0.3524010204457255
1103	76-77	-0.26156395924123643
1103	78-79	-0.31742616048408934
1103	80-81	-0.294909429219679
1103	82-83	-0.2492454884751112
1103	84-85	-0.16013663958246838
1103	86-87	-0.27810150402768485
1103	88-89	-0.20729001057260632
1103	90-91	-0.17441841483398335
1103	92-93	-0.35994232325920805
1103	94-95	-0.015789201795424646
1103	96-97	-0.7650705146544041
1103	98-99	-1.1173666820148433
1103	100	-0.40935805183659824
1104	1	-0.07940138994162993
1104	2	-0.10267612159800876
1104	3	-0.12552400815875586
1104	4	-0.08419363843419347
1104	5	-0.13055617722474722
1104	6	-0.0878140809890553
1104	7	-0.202420822074032
1104	8	-0.09679333797248546
1104	9	-0.1416319035675997
1104	10-11	-0.16079323701261927
1104	12-13	-0.09939317553779148
1104	14-15	-0.21046296731979197
1104	16-17	-0.1229640532182188
1104	18-19	-0.20730189627122542
1104	20-21	-0.25328468164080675
1104	22-23	-0.13763578654327802
1104	24-25	-0.20194577236594569
1104	26-27	-0.15374814510170864
1104	28-29	-0.24728823340434047
1104	30-31	-0.3656108461269696
1104	32-33	-0.25286796732545014
1104	34-35	-0.1491173624467237
1104	36-37	-0.3037752520637227
1104	38-39	-0.3533243374234303
1104	40-41	-0.2373604569463339
1104	42-43	-0.23595089036067662
1104	44-45	-0.40160114243006717
1104	46-47	-0.2917872842328464
1104	48-49	-0.30567150206265836
1104	50-51	-0.19550548783405475
1104	52-53	-0.25219408183853886
1104	54-55	-0.3061461774413985
1104	56-57	-0.3258118554491105
1104	58-59	-0.34519008886201874
1104	60-61	-0.43626304271237615
1104	62-63	-0.27604389541347985
1104	64-65	-0.5132380722032792
1104	66-67	-0.5813385479536635
1104	68-69	-0.42466611933603104
1104	70-71	-0.43224402131756534
1104	72-73	-0.3866779404208316
1104	74-75	-0.41776835434892945
1104	76-77	-0.4413287790721405
1104	78-79	-0.4186276808950993
1104	80-81	-0.20852546401053473
1104	82-83	-0.2179820614611927
1104	84-85	-0.25633057975085194
1104	86-87	-0.2880735549423221
1104	88-89	-0.2708094724827248
1104	90-91	-0.3376737003793515
1104	92-93	-0.45135777076374595
1104	94-95	-0.5425912748791433
1104	96-97	-0.28199474239911737
1104	98-99	-1.1692628189793908
1104	100	-0.5452671848767991
1105	1	-0.08825358721102106
1105	2	-1.084119366146183
1105	3	-0.1759908968566677
1105	4	-0.08777238161520273
1105	5	-0.11466965994323175
1105	6	-0.10494112265794797
1105	7	-0.13771889717797592
1105	8	-0.10052880576188272
1105	9	-0.15245561081817272
1105	10-11	-0.08476811469700607
1105	12-13	-0.0552428277526289
1105	14-15	-0.1678562522934115
1105	16-17	-0.6211869360479554
1105	18-19	-0.13279337157805315
1105	20-21	-0.23867050310691695
1105	22-23	-0.18617870298406558
1105	24-25	-0.23211163971448912
1105	26-27	-0.16155862238593244
1105	28-29	-0.25570866031628725
1105	30-31	-0.15191076250468427
1105	32-33	-0.08484453838217831
1105	34-35	-0.08907326727211284
1105	36-37	-0.20040682301346635
1105	38-39	-0.20482862877999963
1105	40-41	-0.1929356195742713
1105	42-43	-0.26298728042716135
1105	44-45	-0.35315425351157614
1105	46-47	-0.21073053467257097
1105	48-49	-0.19883953080813654
1105	50-51	-0.17335269161573308
1105	52-53	-0.29789903480183
1105	54-55	-0.3238515711867578
1105	56-57	-0.15617126722563057
1105	58-59	-0.2795214316061845
1105	60-61	-0.1389566962518245
1105	62-63	-0.005748685487240834
1105	64-65	-0.1625704938077419
1105	66-67	-0.28211479949052887
1105	68-69	-0.22424367176444804
1105	70-71	-0.259673797126716
1105	72-73	-0.23101406521683288
1105	74-75	-0.1721426309257268
1105	76-77	-0.054478874887568196
1105	78-79	-0.12852307810657493
1105	80-81	0.008833789495433564
1105	82-83	-0.05740093988574557
1105	84-85	-0.02642443069958489
1105	86-87	-0.07708730694697863
1105	88-89	-0.05300805121506613
1105	90-91	-0.10437598027653294
1105	92-93	-0.17624202069471906
1105	94-95	-0.17044298923011425
1105	96-97	-0.1450780751904297
1105	98-99	-0.09313114919302734
1105	100	0.022271214113363413
1106	1	-0.029987131646308285
1106	2	-0.07079230474622022
1106	3	-0.016845940734626197
1106	4	-0.0581585074760298
1106	5	-0.06467588700535742
1106	6	-0.006745125618692782
1106	7	-0.05568048368844103
1106	8	0.007097457924594153
1106	9	-0.05085241766732196
1106	10-11	-0.04193333574076519
1106	12-13	-0.06615518225330419
1106	14-15	-0.15080382626672417
1106	16-17	0.020070612323330295
1106	18-19	-0.061747581914559646
1106	20-21	-0.03906019576115227
1106	22-23	0.010994323773857673
1106	24-25	0.09339836985249406
1106	26-27	0.04543601104438011
1106	28-29	0.01908715747017453
1106	30-31	3.2150512399198306E-4
1106	32-33	0.13625927168555307
1106	34-35	0.13781900888336196
1106	36-37	-0.019438739366194113
1106	38-39	-0.07133584562965467
1106	40-41	-0.003361368942137233
1106	42-43	-0.02063752512764694
1106	44-45	-0.11469413866817035
1106	46-47	-0.15302085282884548
1106	48-49	-0.10564032986776084
1106	50-51	-0.001676410735406364
1106	52-53	-0.007495337611956643
1106	54-55	-0.07818835047492456
1106	56-57	-0.03053970992446864
1106	58-59	-0.14573316659183178
1106	60-61	0.02196516122573655
1106	62-63	-0.03231854761736486
1106	64-65	-0.019165479756615866
1106	66-67	0.033578818509759856
1106	68-69	0.060101927077013784
1106	70-71	-0.023152647284398142
1106	72-73	-0.10272809442538033
1106	74-75	-0.12148410145562849
1106	76-77	-0.06727119026929529
1106	78-79	-0.06441974257580085
1106	80-81	0.1077076184853425
1106	82-83	0.10122431306805879
1106	84-85	0.08688924463486103
1106	86-87	0.01968080544928341
1106	88-89	0.08256570031075228
1106	90-91	0.07738386749509374
1106	92-93	-0.017871884310292074
1106	94-95	-0.013617107771349168
1106	96-97	-0.013808649745399748
1106	98-99	-0.005115509147529451
1106	100	0.033020168244625836
1107	1	-0.020876482000197427
1107	2	-0.07421054417054052
1107	3	-0.04621429861921911
1107	4	-0.0749417230206646
1107	5	-0.03137701410391003
1107	6	-0.10220358459940115
1107	7	-0.0681697796082048
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2316	78-79	-0.05230840080486843
2316	80-81	0.05970158718263008
2316	82-83	-0.05444176879573348
2316	84-85	0.115877953174234
2316	86-87	-0.09449825313915028
2316	88-89	-0.00590947647981821
2316	90-91	-0.02987443746761187
2316	92-93	-0.10303428140247917
2316	94-95	0.0010127428919979309
2316	96-97	0.10802881040475754
2316	98-99	0.25903336391563414
2316	100	-0.0607428526395104
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	7.0
12	48.0
13	205.0
14	674.0
15	2101.0
16	4501.0
17	8863.0
18	14558.0
19	21246.0
20	29223.0
21	39084.0
22	49542.0
23	56467.0
24	61904.0
25	68213.0
26	79059.0
27	93490.0
28	113724.0
29	139640.0
30	172997.0
31	216579.0
32	277421.0
33	373987.0
34	566717.0
35	1058742.0
36	1411046.0
37	1181791.0
38	1145162.0
39	211997.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69806474290079	19.17146932181507	13.166953947235008	23.963511988049127
2	16.387608502958738	21.13962627727907	39.12166802496822	23.35109719479397
3	19.05998697110667	25.911563007674072	30.17830932538985	24.850140695829403
4	13.189094130122347	17.059863242789863	37.54071104342718	32.210331583660604
5	13.43851246844842	37.32619939245811	34.76160819657154	14.47367994252193
6	30.93159439892937	36.73085359751988	18.21034532439052	14.127206679160222
7	26.936387795324805	32.40316152007156	22.616037439153065	18.04441324545056
8	25.648780597245086	35.205315584803664	20.716568143063327	18.429335674887923
9	26.466733233357154	15.71524413252646	20.387058094664535	37.43096453945184
10-11	24.80497455196738	25.843857869982408	28.94454642008077	20.406621157969447
12-13	25.323673052155232	23.744851481488716	28.671839506847764	22.25963595950829
14-15	22.689964820691223	25.258296306098238	26.397274918046282	25.654463955164257
16-17	22.353246463632818	27.912386957043257	27.061712627191802	22.672653952132126
18-19	22.18220754173824	27.09378450593249	28.79796704729906	21.926040905030213
20-21	23.044911811985223	26.379982749201996	28.668963889825193	21.90614154898759
22-23	22.717572036330793	26.25232000589272	28.56430787715702	22.465800080619466
24-25	22.49506203532467	26.30065844259341	28.70262204589629	22.501657476185628
26-27	22.448646516024105	26.450343870365273	28.650369469896425	22.4506401437142
28-29	22.415097068367366	26.672197050154274	28.624424673980513	22.28828120749785
30-31	22.856082777720896	26.395512253209912	28.598957810625116	22.149447158444076
32-33	22.614774195641242	26.52331437438163	28.515856045328476	22.346055384648654
34-35	22.8441385738253	26.595883289265203	28.445298550259224	22.11467958665027
36-37	22.75748546677136	26.546292074527283	28.379212605576793	22.31700985312456
38-39	22.632405955863938	26.726741419290693	28.30508563916251	22.33576698568286
40-41	22.79547984113758	26.506263469144887	28.394954354423756	22.303302335293772
42-43	22.58168715384988	26.647087829929966	28.190125781603143	22.581099234617007
44-45	22.507482177560163	26.804337247035765	28.167196114315818	22.52098446108825
46-47	22.52422054401737	26.61908483705811	27.96133255977546	22.89536205914906
48-49	22.603342508850474	26.7132721747841	27.920771762666476	22.762613553698944
50-51	22.584589049497676	26.96188113883559	27.815170457475404	22.638359354191337
52-53	22.62341180926945	27.00451203123854	27.606454100312188	22.76562205917982
54-55	22.49246961389362	26.823244095875904	27.82786321923854	22.85642307099194
56-57	22.557948558258335	26.989452398068746	27.56100778314927	22.891591260523647
58-59	22.513756113753246	26.887201489087825	27.73166576833396	22.867376628824964
60-61	22.658045986720953	26.877180652464055	27.77687824162457	22.68789511919042
62-63	22.53210449952932	26.940275460424107	27.72652230707735	22.801097732969225
64-65	22.418663503831308	26.738566613052456	27.703439988922597	23.13932989419364
66-67	22.327836255681756	26.813461891164646	27.57618327370511	23.28251857944849
68-69	22.272694127705382	26.841447565426265	27.618167209797562	23.2676910970708
70-71	22.363495289338506	26.63533794193241	27.611825923457644	23.389340845271438
72-73	22.2947572766457	26.8824823315758	27.40142334050552	23.42133705127298
74-75	22.31852320511429	26.937075483441852	27.28760301155779	23.456798299886074
76-77	22.285213109883884	26.812650121286442	27.39385459494302	23.50828217388666
78-79	22.18503886456803	26.795839038110387	27.43391807016572	23.585204027155864
80-81	22.289064747433308	26.938287003946648	27.128412365047993	23.644235883572055
82-83	22.36779721400641	26.912437768718313	27.204442971575123	23.515322045700156
84-85	22.35320997135033	26.945969192211205	27.087724106836593	23.613096729601875
86-87	22.2193351970076	26.95455361289168	27.171373336194982	23.65473785390574
88-89	22.10186683007585	26.79598616734421	27.214670389145144	23.887476613434792
90-91	22.34300163291763	26.72647048408804	27.142667156460863	23.787860726533467
92-93	22.246677995115874	26.776645338313422	27.12020873971881	23.8564679268519
94-95	22.175059845731006	26.664523591219595	27.09503983228058	24.065376730768815
96-97	22.121335603941127	26.600433848274747	27.1882036518942	24.09002689588993
98-99	22.0954732660461	26.572010416444925	27.078675109839295	24.253841207669677
100	22.16883327343577	26.576554963457983	27.19340298750747	24.061208775598775
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2553.0
1	1835.0
2	2364.0
3	3218.5
4	2158.0
5	1628.0
6	2301.0
7	4128.5
8	6193.5
9	7148.0
10	6430.5
11	5375.0
12	5490.0
13	6056.0
14	6690.5
15	7237.5
16	7791.0
17	8499.0
18	9333.5
19	10304.0
20	11516.5
21	13192.0
22	15484.0
23	18783.0
24	23249.5
25	28854.0
26	36254.5
27	45397.0
28	55537.5
29	66550.0
30	78642.0
31	91656.5
32	104881.0
33	117547.0
34	130495.0
35	144443.5
36	162122.5
37	178937.0
38	191518.0
39	202969.5
40	212669.5
41	222679.5
42	233319.0
43	245177.5
44	256141.5
45	265545.0
46	274731.5
47	283774.5
48	294677.5
49	301854.0
50	301538.0
51	295539.0
52	285184.5
53	273260.5
54	259997.0
55	241268.5
56	217932.5
57	195104.0
58	173937.0
59	150511.5
60	125448.0
61	103649.0
62	84476.5
63	65913.0
64	49515.5
65	37625.5
66	28490.5
67	21857.0
68	17061.0
69	13060.0
70	10211.0
71	7987.0
72	6359.5
73	5221.0
74	4147.0
75	3149.5
76	2255.5
77	1471.0
78	962.0
79	637.5
80	433.0
81	275.0
82	182.0
83	128.5
84	80.5
85	50.5
86	33.0
87	24.5
88	20.5
89	16.5
90	9.0
91	4.5
92	5.5
93	5.0
94	1.5
95	1.5
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029044076853141174
2	0.0684002200808504
3	0.002148910292996485
4	1.351515907544959E-5
5	0.001392061384771308
6	0.0011487885214132153
7	0.0
8	3.513941359616894E-4
9	4.0545477226348776E-5
10-11	6.757579537724795E-6
12-13	6.081821583952316E-5
14-15	0.009676853898021908
16-17	0.0011690612600263896
18-19	1.2163643167904632E-4
20-21	2.838183405844414E-4
22-23	0.0019123950091761172
24-25	6.554852151593052E-4
26-27	0.007108973673686486
28-29	0.00779148920699669
30-31	0.009886338863691377
32-33	0.00890648983072128
34-35	0.004432972176747466
36-37	0.0037639718025127113
38-39	0.002784122769542616
40-41	0.0032976988144097002
42-43	0.0016691221458180244
44-45	0.004737063255945082
46-47	0.016853403367085643
48-49	0.01800894946803658
50-51	0.013021855769195683
52-53	0.007386034434733202
54-55	0.001027152089734169
56-57	0.002243516406524632
58-59	0.0027976379286180656
60-61	0.003027395632900709
62-63	0.0028922440421462127
64-65	0.0034125776665510222
66-67	0.0026760014969390193
68-69	0.006534579412979878
70-71	4.324850904143869E-4
72-73	0.0051019725509822205
74-75	0.007244125264440981
76-77	0.006730549219573897
78-79	0.004446487335822915
80-81	4.527578290275613E-4
82-83	0.0011758188395641145
84-85	8.244247036024251E-4
86-87	0.0012839401121677112
88-89	0.001162303680488665
90-91	9.866066125078202E-4
92-93	3.784244541125886E-4
94-95	0.0010136369306587194
96-97	0.0026895166560144687
98-99	0.0029936077352120845
100	0.004811396630860054
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7399099.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.155326059143356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.17841130302841	45.22381844542062
2	14.822356781038684	17.832874102566727
3	4.682674493069714	8.450634328783273
4	1.9167536540693313	4.612117641423804
5	1.029845581235066	3.09753483648817
6	0.576296315011923	2.080037564173501
7	0.3682125226279625	1.550496105042135
8	0.26928673790338287	1.2959225217584862
9	0.18377688103122022	0.9949642380508905
>10	0.9098264443095406	9.455202920724558
>50	0.043407098161076865	1.778526013043729
>100	0.01781789917462801	1.841854862716018
>500	7.709284804646381E-4	0.2988175575885686
>1k	4.744091441145445E-4	0.5920613940688159
>5k	0.0	0.0
>10k+	8.895171449186427E-5	0.8951374681506961
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20634	0.2788717923628269	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18054	0.24400268194816693	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11498	0.1553972990495194	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0545477226348776E-5
2	0.0	0.0	0.0	0.0	4.0545477226348776E-5
3	0.0	0.0	0.0	0.0	4.0545477226348776E-5
4	0.0	0.0	0.0	0.0	4.0545477226348776E-5
5	0.0	0.0	0.0	0.0	4.0545477226348776E-5
6	0.0	0.0	0.0	0.0	4.0545477226348776E-5
7	0.0	0.0	0.0	0.0	4.0545477226348776E-5
8	0.0	0.0	0.0	0.0	4.0545477226348776E-5
9	0.0	0.0	0.0	1.3515159075449592E-5	6.757579537724795E-5
10-11	0.0	0.0	0.0	2.7030318150899184E-5	6.757579537724795E-5
12-13	0.0	6.757579537724796E-6	0.0	4.0545477226348776E-5	6.757579537724795E-5
14-15	0.0	1.3515159075449592E-5	0.0	4.0545477226348776E-5	1.0136369306587193E-4
16-17	1.3515159075449592E-5	1.3515159075449592E-5	0.0	5.406063630179837E-5	1.0812127260359674E-4
18-19	1.3515159075449592E-5	1.3515159075449592E-5	0.0	8.109095445269754E-5	1.1487885214132153E-4
20-21	1.3515159075449592E-5	1.3515159075449592E-5	0.0	1.621819089053951E-4	1.2163643167904632E-4
22-23	1.3515159075449592E-5	1.3515159075449592E-5	0.0	5.203336244048093E-4	1.419091702922207E-4
24-25	2.7030318150899184E-5	1.3515159075449592E-5	0.0	0.0012771825326299865	1.621819089053951E-4
26-27	2.7030318150899184E-5	1.3515159075449592E-5	0.0	0.0024665165312695502	1.621819089053951E-4
28-29	2.7030318150899184E-5	1.3515159075449592E-5	0.0	0.006271033811008611	1.621819089053951E-4
30-31	2.7030318150899184E-5	1.3515159075449592E-5	0.0	0.017481858264094048	1.6893948844311988E-4
32-33	2.7030318150899184E-5	1.3515159075449592E-5	0.0	0.04568123767501962	1.8921222705629428E-4
34-35	2.7030318150899184E-5	2.0272738613174388E-5	0.0	0.09490344702780704	1.8921222705629428E-4
36-37	3.378789768862398E-5	4.0545477226348776E-5	0.0	0.16881109443190312	1.8921222705629428E-4
38-39	4.0545477226348776E-5	5.406063630179837E-5	0.0	0.26990448431626607	2.027273861317439E-4
40-41	4.0545477226348776E-5	5.406063630179837E-5	0.0	0.38163430439300783	2.1624254520719347E-4
42-43	4.7303056764073576E-5	6.757579537724795E-5	0.0	0.49580361068286827	2.1624254520719347E-4
44-45	5.406063630179837E-5	6.757579537724795E-5	0.0	0.6413145708686963	2.3651528382036784E-4
46-47	5.406063630179837E-5	6.757579537724795E-5	0.0	0.8115650297421348	2.838183405844414E-4
48-49	5.406063630179837E-5	8.109095445269755E-5	0.0	0.9892758564252215	2.838183405844414E-4
50-51	5.406063630179837E-5	8.109095445269755E-5	0.0	1.1712574733761503	2.838183405844414E-4
52-53	5.406063630179837E-5	8.109095445269755E-5	0.0	1.3558204857104899	3.1084865873534065E-4
54-55	5.406063630179837E-5	8.109095445269755E-5	0.0	1.5700763025335922	3.378789768862398E-4
56-57	5.406063630179837E-5	8.109095445269755E-5	0.0	1.8168901375694526	3.378789768862398E-4
58-59	5.406063630179837E-5	8.109095445269755E-5	0.0	2.0908964726651176	3.378789768862398E-4
60-61	6.757579537724795E-5	8.109095445269755E-5	0.0	2.377796269518762	3.378789768862398E-4
62-63	6.757579537724795E-5	8.109095445269755E-5	0.0	2.664391975293208	3.64909295037139E-4
64-65	6.757579537724795E-5	8.109095445269755E-5	0.0	2.960874019931346	3.64909295037139E-4
66-67	6.757579537724795E-5	8.109095445269755E-5	0.0	3.295766146661911	3.716668745748638E-4
68-69	6.757579537724795E-5	8.109095445269755E-5	0.0	3.6725214786286813	3.9193961318803816E-4
70-71	6.757579537724795E-5	8.109095445269755E-5	0.0	4.078618220948253	4.3248509041438695E-4
72-73	6.757579537724795E-5	8.784853399042234E-5	0.0	4.469915864080208	4.3248509041438695E-4
74-75	6.757579537724795E-5	9.460611352814714E-5	0.0	4.865997873524871	4.3248509041438695E-4
76-77	6.757579537724795E-5	1.0812127260359674E-4	0.0	5.298962752086436	4.3248509041438695E-4
78-79	6.757579537724795E-5	1.0812127260359674E-4	0.0	5.784129932576926	4.3248509041438695E-4
80-81	6.757579537724795E-5	1.0812127260359674E-4	0.0	6.278561754613635	4.662729881030109E-4
82-83	6.757579537724795E-5	1.0812127260359674E-4	0.0	6.782521763798538	5.338487834802589E-4
84-85	6.757579537724795E-5	1.0812127260359674E-4	0.0	7.292320321704034	5.406063630179836E-4
86-87	6.757579537724795E-5	1.0812127260359674E-4	0.0	7.818438434193136	5.473639425557084E-4
88	6.757579537724795E-5	1.0812127260359674E-4	0.0	8.233191635900534	5.676366811688829E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12380	0.0	47.76433	1
GTATCAA	20175	0.0	35.861782	1
TACCTGG	6705	0.0	30.301176	2
TCAACGC	24970	0.0	28.814112	4
ATCAACG	25240	0.0	28.394741	3
CAACGCA	25605	0.0	28.099527	5
TATCAAC	26035	0.0	27.710367	2
AACGCAG	26805	0.0	27.139252	6
GTACCTG	8160	0.0	26.057777	1
GTACATG	21625	0.0	26.017414	1
TACATGG	22035	0.0	25.142403	2
ACGCAGA	30410	0.0	23.906374	7
ACATGGG	22460	0.0	23.623356	3
CGCAGAG	31600	0.0	22.965523	8
ACCTGGG	8540	0.0	22.89248	3
CATGGGG	16090	0.0	22.022364	4
GCAGAGT	35225	0.0	20.401855	9
ATGGGGG	9630	0.0	20.252117	5
GAGTACT	22110	0.0	19.717287	12-13
AGAGTAC	31135	0.0	19.179964	10-11
>>END_MODULE