FastQCFastQC Report
Mon 6 Jun 2016
ERR522844_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522844_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11420093
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT340530.29818496224155094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334250.29268588268064016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270150.23655674257643963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148450.12999018484350347No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG145200.041.0052222
ACCTGGG163500.035.3802833
GTACCTG212400.028.6073861
TATAACG8900.028.5348642
CCTGGGG170150.024.4891554
GTACATG399400.024.0210651
ATAACGC11900.023.712553
TACATGG402100.023.3803392
CGTATCA53400.022.43851594
ACATGGG412800.022.1477993
CATGGGG267400.022.0198824
ATGGGGG159550.020.3675145
GAGTACT288950.020.03207212-13
CCGTATC70500.018.92885894
TAACGCA14550.018.7472294
CGCCGTA105200.018.35782894
GTACTTT322450.017.7766814-15
AGTACTT302100.017.49535812-13
GCCGTAT90750.017.44931894
AGAGTAC511300.017.39520510-11
Produced by FastQC (version 0.11.5)