FastQCFastQC Report
Mon 6 Jun 2016
ERR522844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11420093
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT593970.5201095998079875No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT342070.2995334626434303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319360.2796474599637674No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT311840.27306257488446023TruSeq Adapter, Index 1 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275540.24127649398301745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147780.12940349960372477No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC115460.1011025041564898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG137450.042.2339552
ACCTGGG163150.034.773193
TATAACG9900.032.760722
GTACCTG210200.028.1964931
CCTGGGG158100.025.8946864
ATAACGC13050.023.4113883
TAACGCA14050.023.0832564
GTACATG392450.022.4257351
TACATGG398850.021.5547812
ACATGGG401600.020.7510173
GAGTACT300600.020.68417212-13
CATGGGG263350.020.3646354
GTATAAC26100.019.2841511
ATGGGGG148250.019.0234975
GTACTTT326900.018.6964414-15
TGGGGGG134750.018.6598226
AGTACTT314500.017.63309712-13
CTGGGGG97750.017.5513255
AGAGTAC518300.017.15141710-11
GTATCAA774600.016.6209011
Produced by FastQC (version 0.11.5)