##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522843_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 693987 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.599759073296763 29.0 27.0 30.0 25.0 30.0 2 32.147786630008916 34.0 34.0 35.0 26.0 35.0 3 31.315275358183943 34.0 33.0 34.0 24.0 34.0 4 31.733892709805804 34.0 32.0 35.0 25.0 35.0 5 32.06038585737197 35.0 34.0 35.0 23.0 36.0 6 32.41487952944364 35.0 34.0 36.0 25.0 36.0 7 32.57265914203004 36.0 33.0 36.0 24.0 36.0 8 33.03910303795316 35.0 35.0 36.0 23.0 38.0 9 33.12338127371262 36.0 34.0 37.0 23.0 38.0 10-11 33.24243825892992 36.0 34.5 37.0 23.0 37.0 12-13 33.460947395268214 36.0 34.5 36.5 23.5 38.5 14-15 33.472877013546366 36.0 34.0 37.0 23.5 38.0 16-17 33.51822800715287 36.5 34.5 37.0 24.5 38.0 18-19 33.55965457566208 36.0 35.0 37.5 24.0 37.5 20-21 33.82517323811541 37.0 34.5 37.5 24.0 38.0 22-23 33.849041120366806 37.0 34.5 38.0 23.5 38.0 24-25 33.6379326990275 36.5 35.0 37.0 23.5 38.0 26-27 33.80182193614578 37.0 35.0 38.0 23.0 38.0 28-29 33.77481350515211 37.0 35.0 37.5 22.5 38.5 30-31 33.81480777017437 37.5 34.0 38.0 23.5 38.5 32-33 33.70542459729073 37.5 34.5 37.5 23.0 38.5 34-35 33.29729951713793 36.5 34.0 37.5 22.5 38.0 36-37 32.99083268130383 36.5 35.0 37.0 20.0 37.5 38-39 33.009938946983155 36.5 34.0 37.5 15.0 38.0 40-41 32.95261294519926 37.0 33.5 37.5 12.0 39.0 42-43 32.21799831985325 36.5 33.0 37.5 11.5 38.0 44-45 31.887474116950315 36.5 32.5 37.5 11.5 38.0 46-47 31.178450749077435 36.0 30.5 37.0 10.5 38.0 48-49 30.779089521849833 36.0 29.5 38.0 10.5 38.0 50-51 30.4560769870329 36.0 29.0 37.0 10.0 38.5 52-53 30.312600956502067 36.0 26.5 37.0 10.0 38.5 54-55 30.35248859128485 36.5 25.0 38.0 10.0 38.5 56-57 30.085516011106836 36.0 23.5 37.5 10.0 38.0 58-59 30.139347711124273 36.5 25.0 37.5 7.0 38.5 60-61 29.769587902943428 36.5 23.5 37.0 8.0 38.5 62-63 29.55807529535856 35.5 23.0 37.0 7.0 38.0 64-65 29.41176779968501 36.0 23.0 37.0 7.0 38.0 66-67 29.5863215016996 36.0 23.0 38.0 7.0 38.0 68-69 29.422089318675997 36.0 23.0 37.5 7.0 38.0 70-71 28.91253150275149 35.5 22.0 37.5 7.0 38.0 72-73 28.963712576748556 36.0 21.5 37.0 7.0 38.0 74-75 28.58551817253061 35.0 22.0 37.0 7.0 38.0 76-77 28.293988215917587 35.0 19.0 37.0 6.5 37.5 78-79 28.161735018091115 35.0 20.5 37.0 7.0 37.5 80-81 27.988582639156064 35.5 19.0 37.0 7.0 37.5 82-83 28.03276286155216 35.0 19.5 37.0 7.0 38.0 84-85 27.58139129407323 35.0 19.5 37.0 6.5 37.5 86-87 27.58054257500501 34.5 15.0 37.0 6.5 38.0 88-89 27.290560918288094 34.5 11.0 37.0 6.5 37.5 90-91 27.05046780415195 35.0 11.0 37.0 6.0 38.0 92-93 26.956891843795344 34.0 11.0 37.0 7.0 37.5 94-95 26.741503082910775 34.0 11.0 37.0 6.0 38.0 96-97 26.61982357018215 33.5 11.5 37.0 7.0 37.5 98-99 26.672604818245873 33.0 12.0 36.5 7.0 37.5 100 26.024252615683004 33.0 11.0 36.0 7.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.015091244365748935 1101 2 0.04311355196485067 1101 3 0.25688663630935693 1101 4 0.24520511194733174 1101 5 0.015274997036243576 1101 6 0.2967279849345914 1101 7 0.08037055498449774 1101 8 -0.16657299849067897 1101 9 0.01715701728600294 1101 10-11 0.11485896280655794 1101 12-13 0.055759768393109255 1101 14-15 -0.004538659227094399 1101 16-17 -0.01573880703497821 1101 18-19 0.13049792040082053 1101 20-21 -0.13864280324215628 1101 22-23 -0.20342462855252563 1101 24-25 0.11039228335333462 1101 26-27 -0.14928609843826024 1101 28-29 -0.19708338471146902 1101 30-31 0.07188536743126406 1101 32-33 -0.11032863248419034 1101 34-35 -0.8272795241204136 1101 36-37 0.16719350822799584 1101 38-39 -0.019352865649828743 1101 40-41 -0.00565317828319678 1101 42-43 -0.28999167982635754 1101 44-45 -0.16039837778557242 1101 46-47 -0.19995650097764184 1101 48-49 0.31422888827982476 1101 50-51 -0.04273759048092174 1101 52-53 -0.00423217446764923 1101 54-55 -0.02493817998860237 1101 56-57 -0.15537276808820621 1101 58-59 -1.2280134078165261 1101 60-61 -0.3520763870260275 1101 62-63 -0.32734727767026683 1101 64-65 -0.4746210913202731 1101 66-67 -0.5402865938740611 1101 68-69 -0.4254573025633306 1101 70-71 -0.5035401132051618 1101 72-73 -0.5314635657350593 1101 74-75 -0.6721871156507824 1101 76-77 -0.7857637123278636 1101 78-79 -0.4476038275233982 1101 80-81 -0.7616454454712347 1101 82-83 -1.4302406788017166 1101 84-85 -0.9293691204488539 1101 86-87 -0.7297549480071801 1101 88-89 -1.423819222618409 1101 90-91 -0.9981337289013403 1101 92-93 -0.8072770818434378 1101 94-95 -1.3714118711097925 1101 96-97 -1.1840479644503183 1101 98-99 -1.2490875253756073 1101 100 -2.5241424137427657 1102 1 0.2283722656095435 1102 2 0.23521690656405525 1102 3 0.5173112162161821 1102 4 0.5944890789544992 1102 5 0.8159486212080438 1102 6 0.2609742756993043 1102 7 0.7766447249393877 1102 8 0.5857816130076401 1102 9 -1.0860869502128594 1102 10-11 0.5285527096566227 1102 12-13 0.43442997527440497 1102 14-15 0.43355675447403286 1102 16-17 0.40772740727093293 1102 18-19 0.2698041366735211 1102 20-21 0.506455597515064 1102 22-23 0.48174457649719216 1102 24-25 0.5072872201088714 1102 26-27 0.39148291484983844 1102 28-29 0.5204259267905371 1102 30-31 0.6317063899140791 1102 32-33 0.30956540692528733 1102 34-35 0.3716756208685794 1102 36-37 0.6071794948784373 1102 38-39 0.3934066578962856 1102 40-41 0.440797387782375 1102 42-43 0.10646441782456151 1102 44-45 0.28743508461808176 1102 46-47 -0.14087179396727834 1102 48-49 0.39562309363686055 1102 50-51 0.22960325351607835 1102 52-53 0.3243218691212455 1102 54-55 0.2503368627275293 1102 56-57 0.46039593788959365 1102 58-59 0.23500855872352844 1102 60-61 0.30764137919688395 1102 62-63 0.5185501298600634 1102 64-65 0.8165418937690312 1102 66-67 0.852051545792321 1102 68-69 0.5425037550757921 1102 70-71 0.6069465433817278 1102 72-73 0.598942944326275 1102 74-75 0.5942029717482633 1102 76-77 0.40907347467659605 1102 78-79 0.5250418682885751 1102 80-81 0.5343718130750652 1102 82-83 0.2742411345748863 1102 84-85 0.10249035269837137 1102 86-87 0.20336818508276266 1102 88-89 0.14882278546999927 1102 90-91 0.06723608831049432 1102 92-93 0.1816781860696821 1102 94-95 0.16986850651309737 1102 96-97 -0.3211710483706156 1102 98-99 -0.7575287246233096 1102 100 -0.3628758036211934 1103 1 0.24316523345594732 1103 2 0.21549680478288735 1103 3 0.25018190786549965 1103 4 0.42815782750874476 1103 5 0.37727177642432963 1103 6 0.28913758821376234 1103 7 0.6283796312544467 1103 8 0.30949199931344396 1103 9 0.34352211756414874 1103 10-11 0.40054553871052434 1103 12-13 0.3568796629912683 1103 14-15 0.33011585694916334 1103 16-17 0.39680328050710756 1103 18-19 0.2868293506452986 1103 20-21 0.2667956391528108 1103 22-23 0.47592685732069384 1103 24-25 0.40832816389790594 1103 26-27 0.17916508001292186 1103 28-29 -0.13503902810189317 1103 30-31 0.47200248059186123 1103 32-33 0.21610600373306 1103 34-35 0.3488037726150637 1103 36-37 0.46055099615070105 1103 38-39 0.6368834101516612 1103 40-41 0.20925679097459948 1103 42-43 0.2964597914077203 1103 44-45 0.19670526736155125 1103 46-47 0.15210761847778542 1103 48-49 0.4095370422836311 1103 50-51 0.34050424819570324 1103 52-53 0.3339101180224695 1103 54-55 0.19303869016217945 1103 56-57 0.47474361311513036 1103 58-59 0.3356851721864018 1103 60-61 0.5673973357085664 1103 62-63 0.2826549182014908 1103 64-65 0.4387371285596835 1103 66-67 0.26821508962762053 1103 68-69 0.059951129584234764 1103 70-71 0.06474783877844104 1103 72-73 -0.020557402654986845 1103 74-75 0.14619233098953188 1103 76-77 -0.5497514154460035 1103 78-79 -0.20715220989787042 1103 80-81 0.4140791922099254 1103 82-83 0.10944238370743165 1103 84-85 -0.27184459988085763 1103 86-87 -0.455907780681752 1103 88-89 -0.11868820226999688 1103 90-91 -0.20203872084981 1103 92-93 0.027231042882078782 1103 94-95 -0.32522170861528465 1103 96-97 -0.7601312611857871 1103 98-99 -0.40399529876163953 1103 100 -0.5980481376484903 1104 1 0.04574121483547344 1104 2 -0.09545838970258558 1104 3 -0.06606176967802924 1104 4 -0.2028904693445739 1104 5 0.052407177704488106 1104 6 0.1354000652738776 1104 7 0.06148692278123491 1104 8 -0.20261578458163143 1104 9 0.06455519576118007 1104 10-11 -0.17238203838987687 1104 12-13 -0.07828214713361348 1104 14-15 -0.21414245736085036 1104 16-17 0.08760382658830679 1104 18-19 0.2310171667681189 1104 20-21 -0.04653187528253255 1104 22-23 0.08352499527334345 1104 24-25 0.025637147381189607 1104 26-27 -0.2990207666367368 1104 28-29 -0.14954486938026434 1104 30-31 -0.07520668641051742 1104 32-33 -0.7248601571370941 1104 34-35 -0.195266546529119 1104 36-37 0.06373908083804736 1104 38-39 -0.07947888338936338 1104 40-41 0.08009739793041604 1104 42-43 0.02263364217537145 1104 44-45 0.2068156026195993 1104 46-47 0.022661756609469563 1104 48-49 -0.2562142669684171 1104 50-51 -0.28700459205316875 1104 52-53 -0.26675010309565295 1104 54-55 -0.3731400842883801 1104 56-57 -0.3776163976274489 1104 58-59 -0.16090741543277076 1104 60-61 -0.2499194809066303 1104 62-63 -0.2931776318186472 1104 64-65 -0.27938873867991987 1104 66-67 1.1495982143472361E-4 1104 68-69 -0.2402626030150259 1104 70-71 -0.39238143027844785 1104 72-73 -0.5467424436110733 1104 74-75 -0.04718979156541181 1104 76-77 -0.2790025680354127 1104 78-79 -0.3471761569011349 1104 80-81 -0.21929106874526738 1104 82-83 -0.621291468850643 1104 84-85 -0.8770793700343873 1104 86-87 -0.56419075816709 1104 88-89 -0.4734104617928452 1104 90-91 -0.3650741713667962 1104 92-93 -0.46324722577196553 1104 94-95 -0.5058212789120518 1104 96-97 -0.6956768947742589 1104 98-99 -0.2605404399818205 1104 100 0.3961486306250883 1105 1 0.06404113949035306 1105 2 -0.042476298002895874 1105 3 0.06978718251400196 1105 4 -0.12002573724542387 1105 5 0.19014401465679498 1105 6 0.32781106651260217 1105 7 0.397006825385958 1105 8 -0.14208428223401626 1105 9 0.5067480366978501 1105 10-11 -0.17041114629515874 1105 12-13 0.17862448877067294 1105 14-15 -0.020641718808413145 1105 16-17 0.057241233524308655 1105 18-19 -0.03603764787012409 1105 20-21 0.07551976804985117 1105 22-23 0.07562120715913778 1105 24-25 0.15376961005981116 1105 26-27 -0.02827531820557283 1105 28-29 -0.03653889710897573 1105 30-31 0.22067508560451898 1105 32-33 0.2750855688365448 1105 34-35 -0.0635466771273272 1105 36-37 0.05020037175108172 1105 38-39 -0.02443437868773657 1105 40-41 -0.159448998912751 1105 42-43 -0.2132817158842144 1105 44-45 -0.23385756557880555 1105 46-47 -0.3403559218243757 1105 48-49 0.0688637386420261 1105 50-51 -0.1344913853734191 1105 52-53 -0.19389094738666302 1105 54-55 0.2274679976462437 1105 56-57 -0.12193571620108301 1105 58-59 0.0865693744394811 1105 60-61 0.24946222087143255 1105 62-63 0.4055983292572982 1105 64-65 0.043431041755038535 1105 66-67 -0.001968737112402863 1105 68-69 0.15506727664562447 1105 70-71 0.2708673916559192 1105 72-73 0.07028619179091322 1105 74-75 0.2912364319414138 1105 76-77 0.2796346643723524 1105 78-79 -0.07472054454112964 1105 80-81 0.43989571478437384 1105 82-83 0.39775126502784275 1105 84-85 -0.04055912262996131 1105 86-87 0.14695223154425818 1105 88-89 0.05884214442461655 1105 90-91 -0.5640704211379948 1105 92-93 0.049410328533987524 1105 94-95 -0.34616092789744357 1105 96-97 0.4600001683836794 1105 98-99 0.13381144300763737 1105 100 -0.04308787216949028 1106 1 0.07245595728815601 1106 2 -0.0018976639216248259 1106 3 -0.3393906738468182 1106 4 0.07719183571305877 1106 5 -0.12432938805893201 1106 6 -0.20312786103858826 1106 7 -0.3498061567566708 1106 8 0.00871126621830598 1106 9 0.1236200400201497 1106 10-11 -0.19488584940645381 1106 12-13 0.0683855515713887 1106 14-15 -0.02646413511117629 1106 16-17 0.03633947211753252 1106 18-19 -0.29320240798908515 1106 20-21 -0.08047841139634926 1106 22-23 -0.05722783369425599 1106 24-25 -0.2200825811067233 1106 26-27 -0.17071582510816086 1106 28-29 -0.022927740553932097 1106 30-31 -0.19418780655913537 1106 32-33 -0.1550234127020289 1106 34-35 -0.13780136169749824 1106 36-37 -0.03613750457710552 1106 38-39 -0.1913883908275622 1106 40-41 -0.28020331224096395 1106 42-43 0.03592651100544941 1106 44-45 0.03511667400439933 1106 46-47 0.28262898939216896 1106 48-49 0.009993572652824412 1106 50-51 -0.06639994659058956 1106 52-53 0.14965049118642426 1106 54-55 0.2536447507682382 1106 56-57 0.3280881942981395 1106 58-59 0.36944526216919726 1106 60-61 0.04841162709403335 1106 62-63 0.4349673437671946 1106 64-65 0.4335035702693588 1106 66-67 0.3769057075977109 1106 68-69 0.7529807709763219 1106 70-71 0.669177506636057 1106 72-73 0.5671096609946105 1106 74-75 0.48270814414290086 1106 76-77 0.569224368595286 1106 78-79 0.7928907965861853 1106 80-81 0.46957409759566104 1106 82-83 0.48776380755287363 1106 84-85 0.46524022213846195 1106 86-87 0.27632061683048903 1106 88-89 0.7654655624514568 1106 90-91 0.17623638832049338 1106 92-93 0.5674354890908937 1106 94-95 0.09860057535983913 1106 96-97 0.34263107836694573 1106 98-99 0.48646718635150066 1106 100 0.4528913886185464 1107 1 0.10978302426728703 1107 2 -0.08865764291994793 1107 3 0.20316333504047535 1107 4 -0.1996525341725821 1107 5 0.10415349685443687 1107 6 -0.2000608477588699 1107 7 0.18864356087321 1107 8 -0.021397541253513452 1107 9 0.01145955646932606 1107 10-11 -0.1496255267831259 1107 12-13 0.08063332868041329 1107 14-15 -0.0968880531192653 1107 16-17 0.026593114329202194 1107 18-19 -0.2639526630399516 1107 20-21 -0.18569702946889066 1107 22-23 0.18638629036077958 1107 24-25 -0.10398855164461906 1107 26-27 5.727046463519514E-4 1107 28-29 0.34348003348167566 1107 30-31 0.10460267609527563 1107 32-33 0.21207374566854043 1107 34-35 0.023559393338423718 1107 36-37 -0.38282632545242024 1107 38-39 -0.1563717218131515 1107 40-41 0.12113362381916915 1107 42-43 -0.14039260350919136 1107 44-45 -0.21763979617706042 1107 46-47 -0.12462747438634025 1107 48-49 -0.12213451196856795 1107 50-51 -0.24260914378865905 1107 52-53 0.06577717569598107 1107 54-55 0.16322441841242608 1107 56-57 0.4495969855491495 1107 58-59 -0.24972538499834585 1107 60-61 0.12724582055704659 1107 62-63 0.32686312934518824 1107 64-65 -0.024801483366022836 1107 66-67 -0.17081228085136146 1107 68-69 -0.21763929074812438 1107 70-71 -0.4603499129322124 1107 72-73 -0.6840471387253686 1107 74-75 -0.4195945702812409 1107 76-77 -0.29129589247435206 1107 78-79 -0.44745035261536614 1107 80-81 -0.36316909713513823 1107 82-83 -0.6319799036825984 1107 84-85 -0.6793714816012866 1107 86-87 -0.7544903808772538 1107 88-89 -0.6683460712827021 1107 90-91 -0.8153085155712176 1107 92-93 -0.9005060050162612 1107 94-95 -0.5642979549495948 1107 96-97 -0.5788751517038726 1107 98-99 -0.8076854258388657 1107 100 -0.9616356254295262 1108 1 0.07992357315546172 1108 2 -0.12862084227593584 1108 3 -0.13227289223635808 1108 4 -0.04134815670311909 1108 5 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0.6864768501129994 2316 100 0.5116651780814259 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 33.0 1 20.0 2 9.0 3 14.0 4 12.0 5 21.0 6 21.0 7 65.0 8 102.0 9 97.0 10 122.0 11 153.0 12 184.0 13 642.0 14 2426.0 15 5823.0 16 10271.0 17 15458.0 18 19925.0 19 23002.0 20 25578.0 21 28569.0 22 29222.0 23 21912.0 24 14530.0 25 11168.0 26 9105.0 27 8244.0 28 8574.0 29 8840.0 30 10529.0 31 14510.0 32 20981.0 33 32115.0 34 52380.0 35 98336.0 36 180589.0 37 37784.0 38 2621.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.4137518555349 16.76927954804214 14.34505022554657 15.47191837087639 2 15.838997772667968 25.296148661798735 32.16108872566333 26.703764839869965 3 30.410204272931136 25.47362045469294 30.836632128461932 13.279543143913994 4 12.86360679477915 16.531587472612745 39.20628532937543 31.39852040323267 5 9.85948403437463 39.86949410213731 33.128275989395675 17.142745874092384 6 24.89830936159475 33.564471360080745 15.150715547384738 26.386503730939765 7 19.95458215583367 36.328363804140956 22.58939385200992 21.127660188015458 8 24.82864220030047 21.147729599266412 21.055310289542057 32.96831791089106 9 22.55299148503032 21.707405687988214 26.360073932889467 29.379528894092 10-11 25.71893319265291 22.93494539264397 23.715894726069553 27.63022668863357 12-13 33.520870541666845 23.10109567280595 24.163902619034232 19.21413116649297 14-15 26.69185505098702 24.660735239424195 32.20802472517176 16.43938498441703 16-17 17.943027093810887 28.70666644561235 31.655074530145445 21.695231930431323 18-19 17.5658092499508 31.760293361802557 31.155923066775564 19.517974321471076 20-21 22.304784477911475 24.532451579976296 28.344636384868906 24.818127557243326 22-23 18.871406287393206 25.4944913946964 31.368847330467304 24.26525498744309 24-25 25.004180722544184 25.845154686734134 27.86703860681025 21.283625983911424 26-27 21.99334092132873 21.353238966350677 36.73795970385193 19.915460408468665 28-29 17.738024530467026 31.846719179780532 31.17673007836602 19.23852621138643 30-31 20.009456510144126 25.56717437747044 30.19315210277612 24.230217009609316 32-33 19.659817368053105 26.67615622454937 33.37724956576263 20.286776841634897 34-35 22.301715998933354 25.04623323459673 31.172659329888365 21.47939143658155 36-37 19.90634276271554 23.910340995652245 36.24245376772919 19.940862473903024 38-39 19.900622997956972 26.659916621326058 33.551016290189494 19.888444090527475 40-41 18.48365278243228 28.976141314335752 31.705566305957646 20.834639597274325 42-43 17.837266973283928 28.696131720468866 27.85694718781989 25.60965411842731 44-45 20.32790761719792 24.144364709631187 30.1016222357757 25.426105437395197 46-47 23.137143499865893 28.013794237163975 30.790883056131555 18.058179206838574 48-49 20.915199044723774 26.204767151419357 31.08527109424123 21.79476270961564 50-51 14.653533309154726 27.85519658576928 35.34859373524137 22.142676369834614 52-53 18.99625685258401 29.9505864168159 26.838770701941073 24.214386028659014 54-55 17.041607552741155 24.589518668466038 29.83234714003945 28.536526638753358 56-57 21.997849170950545 22.460666641679378 28.431956245332902 27.109527942037175 58-59 25.483743801182364 22.264430515947744 28.1900713697324 24.06175431313749 60-61 20.055345168647825 23.96265642396705 33.419185604491034 22.56281280289409 62-63 16.94973026806008 24.42151398671446 33.018778185654554 25.609977559570908 64-65 16.577077269323816 28.745981448905216 29.71879330684687 24.958147974924096 66-67 19.316851749348462 23.84950356327406 27.112883138448762 29.72076154892871 68-69 17.51445778269794 23.44719377753177 30.226279434488802 28.812069005281487 70-71 22.18228625750361 23.2787915522822 28.436128602615206 26.10279358759898 72-73 19.76180215136215 19.71394926152325 34.983633446863834 25.540615140250765 74-75 17.372365791985146 26.7380222644458 29.973603858939946 25.916008084629112 76-77 18.773037264690153 21.74119556646625 30.313832953973762 29.17193421486984 78-79 19.934120305747502 21.813226526845867 32.05181882579642 26.200834341610214 80-81 22.185110962666073 20.752400161704205 30.387780235047586 26.674708640582136 82-83 19.9006735377558 19.657617706973966 33.64914498103761 26.792563774232626 84-85 19.933489700991892 22.287939874113228 31.418081721917773 26.360488702977108 86-87 20.31007472635167 22.81776714490571 30.05094650976775 26.82121161897487 88-89 20.22188370561353 22.413266328182086 27.4458585599465 29.918991406257884 90-91 22.1373952283735 19.326478152805333 28.659881628149815 29.876244990671353 92-93 24.14247897503238 21.17919996655897 29.068400603379995 25.60992045502866 94-95 22.087747911235905 19.86345648387113 30.019538133232626 28.02925747166034 96-97 19.316955052212077 20.31629924834788 32.917822731170865 27.448922968269184 98-99 22.434114705884472 21.95429377753403 27.97821563979847 27.633375876783024 100 21.830412416455147 16.411329669395162 29.452614555722274 32.30564335842742 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4292.0 1 2431.0 2 561.0 3 514.0 4 389.0 5 363.0 6 586.5 7 1043.0 8 1355.0 9 1210.0 10 808.5 11 504.5 12 439.5 13 424.0 14 450.0 15 507.0 16 547.5 17 615.0 18 754.0 19 948.5 20 1220.5 21 1593.5 22 2095.0 23 2752.0 24 3647.5 25 4883.0 26 6267.5 27 7696.5 28 9126.0 29 10370.5 30 11359.0 31 11777.5 32 11568.5 33 10940.0 34 9738.5 35 8284.5 36 6990.0 37 5816.0 38 4774.0 39 4071.0 40 3689.0 41 3515.5 42 3546.5 43 3713.5 44 4073.0 45 5833.5 46 16245.0 47 48360.5 48 103406.5 49 113559.0 50 73379.5 51 42994.0 52 24533.0 53 13807.5 54 8225.0 55 6251.5 56 5605.0 57 5330.0 58 5152.0 59 5018.5 60 4803.0 61 4540.5 62 4251.0 63 3950.0 64 3691.5 65 3427.0 66 3118.0 67 2877.0 68 2549.0 69 2235.5 70 2005.5 71 1727.5 72 1466.0 73 1224.5 74 977.0 75 797.0 76 666.0 77 532.5 78 399.0 79 280.0 80 211.5 81 155.5 82 104.0 83 70.5 84 46.5 85 30.0 86 15.5 87 11.5 88 9.0 89 6.5 90 3.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016859105429928804 2 0.047839512843900533 3 0.07954039484889486 4 0.09942549356111857 5 0.09870501897009598 6 0.0665718522104881 7 0.06152853007332991 8 0.05893482154564855 9 0.05547654350874008 10-11 0.031124502332176254 12-13 0.03717648889676608 14-15 0.04049067201547003 16-17 0.049208414566843474 18-19 0.053675357031183576 20-21 0.053171024817467764 22-23 0.049712746780559286 24-25 0.047119038252877936 26-27 0.05007298407607058 28-29 0.045966278907241774 30-31 0.04106705168828811 32-33 0.03487097020549376 34-35 0.03198907184140337 36-37 0.0260091327359158 38-39 0.023703614044643488 40-41 0.030908359954869468 42-43 0.05720568252719432 44-45 0.06311357417357961 46-47 0.07334431336610052 48-49 0.08285457796759882 50-51 0.07730692361672481 52-53 0.06527499794664741 54-55 0.05922301138205759 56-57 0.043372570379560424 58-59 0.029611505691028796 60-61 0.022983139453620887 62-63 0.021614237730677954 64-65 0.025000468308484163 66-67 0.035015065123698286 68-69 0.035015065123698286 70-71 0.03422254307357342 72-73 0.02795441413167682 74-75 0.046830848416468895 76-77 0.039482007588038395 78-79 0.021109905516962135 80-81 0.018372102071076257 82-83 0.016354773216212985 84-85 0.015778393543394904 86-87 0.0175795800209514 88-89 0.015850441002497166 90-91 0.02255085469900733 92-93 0.03292568880973275 94-95 0.03105245487307399 96-97 0.026225275113222583 98-99 0.02687370224514292 100 0.028530793804494897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 693987.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.491039338809074 #Duplication Level Percentage of deduplicated Percentage of total 1 89.53166868269535 30.880403066040657 2 4.836761572005914 3.3364986730499195 3 1.7029605346030208 1.7621063637429633 4 0.9160080177363793 1.2637627429763991 5 0.5771442125622261 0.9953151869824862 6 0.41871670045082693 0.8665184512239481 7 0.297627194041015 0.7185829880577607 8 0.21292614414865269 0.5875235211273689 9 0.1730703074627593 0.5372437304770031 >10 1.0755641146643882 7.178146704871866 >50 0.11358939880287354 2.748355395926763 >100 0.11192508526694647 7.937811793595145 >500 0.013314508284543818 3.17343130228105 >1k 0.011234116365083846 9.368173562230574 >5k 0.004992940606703931 13.12784223030632 >10k+ 0.0024964703033519657 15.518284287109802 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35964 5.182229638307346 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 24847 3.5803264326277007 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 15632 2.2524917613730517 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11248 1.6207796399644374 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10417 1.5010367629364816 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10026 1.4446956499185144 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9598 1.3830230249269797 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9510 1.370342672124982 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9387 1.3526189971858262 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8808 1.2691880395454094 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8282 1.193394112569832 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 7058 1.0170219326874999 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7038 1.0141400343234095 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7020 1.0115463257957282 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6638 0.9565020670416017 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6325 0.9114003576435871 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6214 0.8954058217228854 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5599 0.8067874470271057 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4887 0.7041918652654877 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4655 0.6707618442420391 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3803 0.5479929739317884 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3791 0.5462638349133341 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3665 0.5281078752195647 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3596 0.5181653258634528 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3345 0.4819975013941183 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3318 0.4781069386025963 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3264 0.4703258130195522 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3090 0.44525329725196583 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3090 0.44525329725196583 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2256 0.3250781354693964 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1997 0.2877575516544258 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1857 0.2675842631057931 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1735 0.2500046830848417 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 1702 0.2452495507840925 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1603 0.23098415388184504 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1550 0.22334712321700553 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1527 0.22003294009830154 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1469 0.21167543484243942 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1418 0.20432659401400888 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1368 0.19712184810378292 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1318 0.18991710219355692 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1275 0.1837210207107626 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1271 0.1831446410379445 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1245 0.179398173164627 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1184 0.1706083831541513 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 987 0.14222168426786091 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 973 0.14020435541299764 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 935 0.1347287485212259 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 933 0.13444055868481686 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 865 0.12464210424690952 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 864 0.12449800932870501 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 856 0.12334524998306884 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC 837 0.12060744653718297 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 831 0.11974287702795586 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 798 0.11498774472720671 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 752 0.1083593784897988 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 750 0.10807118865338976 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.3228475461355904E-4 0.0 10-11 0.0 0.0 0.0 5.763796728180787E-4 0.0 12-13 0.0 0.0 0.0 6.484271319203386E-4 0.0 14-15 0.0 0.0 0.0 0.0010086644274316379 0.0 16-17 0.0 0.0 0.0 0.0012968542638406772 0.0 18-19 0.0 0.0 0.0 0.002665755986783614 0.0 20-21 0.0 0.0 0.0 0.012968542638406771 7.204745910225984E-5 22-23 0.0 0.0 0.0 0.04870408235312765 1.4409491820451968E-4 24-25 0.0 0.0 0.0 0.1072786666032649 1.4409491820451968E-4 26-27 0.0 0.0 0.0 0.20158879056812304 1.4409491820451968E-4 28-29 0.0 0.0 0.0 0.492156193127537 1.4409491820451968E-4 30-31 0.0 0.0 0.0 0.9922376067563226 1.4409491820451968E-4 32-33 0.0 0.0 0.0 1.8243857593874235 1.4409491820451968E-4 34-35 0.0 0.0 0.0 2.9847100882293187 1.4409491820451968E-4 36-37 0.0 0.0 0.0 4.29157894888521 2.1614237730677952E-4 38-39 0.0 0.0 0.0 5.778422362378546 2.8818983640903936E-4 40-41 0.0 0.0 0.0 7.180898201263136 2.8818983640903936E-4 42-43 0.0 0.0 0.0 8.416295982489586 2.8818983640903936E-4 44-45 0.0 0.0 0.0 9.712069534443728 2.8818983640903936E-4 46-47 0.0 1.4409491820451968E-4 0.0 11.193509388504395 2.8818983640903936E-4 48-49 0.0 1.4409491820451968E-4 0.0 12.321268265832067 2.8818983640903936E-4 50-51 0.0 1.4409491820451968E-4 0.0 13.337929961224058 2.8818983640903936E-4 52-53 0.0 1.4409491820451968E-4 0.0 14.221087714899559 2.8818983640903936E-4 54-55 0.0 1.4409491820451968E-4 0.0 15.203454819758871 2.8818983640903936E-4 56-57 0.0 1.4409491820451968E-4 0.0 16.551606874480356 2.8818983640903936E-4 58-59 0.0 1.4409491820451968E-4 0.0 17.457027293018456 2.8818983640903936E-4 60-61 0.0 1.4409491820451968E-4 0.0 18.323614131100438 2.8818983640903936E-4 62-63 0.0 1.4409491820451968E-4 0.0 19.121899977953476 2.8818983640903936E-4 64-65 0.0 1.4409491820451968E-4 0.0 19.90750547200452 2.8818983640903936E-4 66-67 0.0 1.4409491820451968E-4 0.0 20.864728013637144 2.8818983640903936E-4 68-69 0.0 1.4409491820451968E-4 0.0 21.95091550706281 2.8818983640903936E-4 70-71 0.0 1.4409491820451968E-4 0.0 22.964839399008916 2.8818983640903936E-4 72-73 0.0 1.4409491820451968E-4 0.0 23.72140976704175 2.8818983640903936E-4 74-75 0.0 1.4409491820451968E-4 0.0 24.647723948719502 2.8818983640903936E-4 76-77 0.0 1.4409491820451968E-4 0.0 25.35840008530419 2.8818983640903936E-4 78-79 0.0 1.4409491820451968E-4 0.0 26.607991215973787 2.8818983640903936E-4 80-81 0.0 2.1614237730677952E-4 0.0 27.681066071842842 2.8818983640903936E-4 82-83 0.0 2.8818983640903936E-4 0.0 28.470273938848997 2.8818983640903936E-4 84-85 0.0 2.8818983640903936E-4 0.0 29.359483679089088 2.8818983640903936E-4 86-87 0.0 2.8818983640903936E-4 0.0 30.136659620425164 2.8818983640903936E-4 88 0.0 4.3228475461355904E-4 0.0 30.831413268548257 2.8818983640903936E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 465 0.0 56.624477 1 GTACGGG 25 0.0052270005 56.42225 1 ACCCATG 555 0.0 48.28931 3 TACCCAT 595 0.0 45.8332 2 GCTTATA 150 0.0 43.883972 1 CACCTAT 50 0.0016982682 37.614834 1 ACATGAG 60 0.004151831 31.345694 3 ATCATTA 525 0.0 29.537466 94 GTATAAC 70 0.0088166725 26.867737 1 TCAAGCA 70 0.0088166725 26.867737 4 AATTGAG 35 0.008839846 26.855145 70-71 TATGGGA 130 1.0836959E-5 25.317677 4 CATTAAA 335 0.0 25.24912 94 ATATGGG 135 1.45191425E-5 24.379984 3 ATGGGCA 235 2.0008883E-10 24.007736 5 GCTTATG 530 0.0 23.95284 1 TATATGG 140 1.9240033E-5 23.50927 2 TCATTAA 405 0.0 23.20564 94 CGTATCA 975 0.0 22.652273 94 GAGTACT 5830 0.0 21.967424 12-13 >>END_MODULE