##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522843_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 693987 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.85255775684559 34.0 32.0 35.0 29.0 36.0 2 31.592067286563005 33.0 31.0 34.0 28.0 34.0 3 32.583271732755804 34.0 31.0 35.0 30.0 35.0 4 33.44787150191574 35.0 32.0 36.0 30.0 36.0 5 34.35126882780225 36.0 35.0 36.0 30.0 36.0 6 34.631147269329254 36.0 34.0 36.0 31.0 37.0 7 34.69961108781577 36.0 35.0 36.0 32.0 38.0 8 34.62375664097454 36.0 35.0 36.0 31.0 37.0 9 34.73346330694955 36.0 35.0 36.0 31.0 37.0 10-11 34.71119199639186 36.0 34.5 36.5 31.5 37.5 12-13 34.7340295999781 36.0 34.0 36.5 30.5 37.0 14-15 35.241797036543915 37.0 34.0 37.5 31.0 38.0 16-17 35.376250563771364 37.0 35.0 37.0 31.5 37.5 18-19 35.350393451174156 36.5 35.0 37.5 31.0 39.0 20-21 35.36798095641561 37.0 35.0 37.5 31.0 38.0 22-23 35.39097562346269 36.5 35.0 37.5 31.5 37.5 24-25 35.38210441982342 36.5 35.0 37.5 31.5 38.0 26-27 35.34676370018458 36.5 36.0 37.0 29.5 38.0 28-29 35.09613796800228 37.0 34.5 37.0 30.5 38.0 30-31 35.04538485591229 37.0 35.0 37.0 29.5 37.5 32-33 34.99134926158559 36.5 35.5 37.0 29.5 38.0 34-35 34.711547910839826 36.5 35.0 37.0 28.5 38.5 36-37 34.48586428852414 36.0 35.0 37.0 28.0 38.0 38-39 34.39370982453562 37.0 35.0 37.0 28.0 37.5 40-41 34.450396765357276 36.5 34.5 37.5 28.5 38.0 42-43 33.97564435645049 36.0 34.0 37.5 27.0 38.0 44-45 33.62071335630206 36.5 34.0 37.0 26.0 38.0 46-47 33.062732443114925 36.0 34.0 37.0 20.5 37.5 48-49 32.70067955163425 36.5 33.0 37.0 14.5 38.0 50-51 32.32613651264361 36.5 33.0 37.0 13.5 38.0 52-53 32.10900852609631 36.5 32.5 37.0 14.0 37.5 54-55 32.22174550820117 37.0 33.5 37.0 13.5 37.5 56-57 31.881385386181584 36.5 32.0 37.0 13.0 38.5 58-59 31.49868081102384 36.5 31.0 37.0 12.5 38.0 60-61 30.870758385964002 35.5 29.5 37.0 12.0 37.0 62-63 30.98827139413274 36.5 26.5 37.0 13.0 37.0 64-65 30.54281708446988 36.5 28.0 37.0 11.5 37.5 66-67 30.027477459952422 36.0 25.5 37.0 11.5 37.5 68-69 29.377458799660513 35.5 25.0 37.0 9.5 37.5 70-71 28.904387978449165 35.5 24.0 37.0 7.0 37.5 72-73 28.634454247702045 35.5 22.0 37.0 8.5 37.5 74-75 28.608752037141905 36.0 22.0 37.0 7.0 37.0 76-77 28.77861761099271 35.5 23.5 36.5 8.0 37.0 78-79 28.744386422224046 35.5 23.0 37.0 8.0 37.0 80-81 28.576248546442514 35.5 24.0 37.0 7.5 38.0 82-83 28.54166720702261 35.5 22.5 37.0 7.0 37.0 84-85 28.69054463556234 36.0 23.0 37.0 7.0 37.0 86-87 28.63620644190741 35.5 22.0 37.0 7.0 38.0 88-89 28.37951503414329 35.0 22.0 37.0 7.5 37.5 90-91 28.398771879012145 35.5 21.0 37.0 7.0 38.0 92-93 28.425269493520773 35.5 21.0 37.0 7.0 37.5 94-95 28.26631910972396 35.5 22.0 37.0 7.0 37.0 96-97 28.024803058270543 35.5 22.0 37.0 7.0 37.0 98-99 28.11655405648809 34.5 21.0 37.0 7.5 37.5 100 27.765876017850477 34.0 19.0 37.0 7.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11408636115675108 1101 2 0.29658532196761556 1101 3 -0.022225089132120956 1101 4 0.04053231439513638 1101 5 -0.004711517655124453 1101 6 0.2010615459906333 1101 7 0.03859055663353672 1101 8 0.03344189079984261 1101 9 0.007913514379822573 1101 10-11 -0.004428926942800615 1101 12-13 0.09615773442489228 1101 14-15 0.05168286733986349 1101 16-17 -0.0033269779428692914 1101 18-19 0.06803097192609897 1101 20-21 -0.1774214272844148 1101 22-23 -0.05659501851720705 1101 24-25 -0.0455210576133922 1101 26-27 0.10113291517841105 1101 28-29 -0.037162271633597754 1101 30-31 -0.11179960762224539 1101 32-33 -0.046409873595798956 1101 34-35 -0.13536074636288475 1101 36-37 -0.27324041541189104 1101 38-39 -0.0731517598849436 1101 40-41 -0.43842491917204285 1101 42-43 -0.18833421895266866 1101 44-45 -0.26800607974023194 1101 46-47 -0.04048575855733105 1101 48-49 -0.5225390325509096 1101 50-51 -0.593014719857063 1101 52-53 -0.7373906734565097 1101 54-55 -1.118322470534583 1101 56-57 -0.5551978209918502 1101 58-59 -0.4938546672261239 1101 60-61 -0.5145188977518984 1101 62-63 -0.7231384569324604 1101 64-65 -1.304353985334842 1101 66-67 -0.6943185512073242 1101 68-69 -0.5871506452450639 1101 70-71 -0.38642420436390523 1101 72-73 -0.4173852772289166 1101 74-75 -0.6524383858702549 1101 76-77 -0.0012955571006507682 1101 78-79 -0.31274445480346813 1101 80-81 -0.39996288656340084 1101 82-83 -0.44083534937492885 1101 84-85 -0.3646204349689981 1101 86-87 -0.022361094797741288 1101 88-89 -0.8644949825496546 1101 90-91 -0.7617264020950998 1101 92-93 -0.7206727333454346 1101 94-95 -0.3213448394159464 1101 96-97 -0.780400468493827 1101 98-99 -0.9368215189928009 1101 100 -0.3702707484764751 1102 1 0.1401020216895006 1102 2 0.2865842156505458 1102 3 0.09397508050316361 1102 4 0.05761876692798751 1102 5 0.06739331203350929 1102 6 0.3087184893594923 1102 7 0.42515249195086824 1102 8 0.4104649144873207 1102 9 0.20152391867725328 1102 10-11 -0.5156408358314408 1102 12-13 0.33652478584405543 1102 14-15 0.08464496983398817 1102 16-17 0.12888528742571737 1102 18-19 0.010629096104047164 1102 20-21 0.2971972006365675 1102 22-23 0.19755428511765416 1102 24-25 0.1818492267100993 1102 26-27 0.12887320109991407 1102 28-29 0.033863284290738704 1102 30-31 0.1622019412216531 1102 32-33 -0.1417485910055376 1102 34-35 0.22380754904835953 1102 36-37 0.17433736482437467 1102 38-39 0.14600842253445734 1102 40-41 0.20720943074375242 1102 42-43 0.32740498523659056 1102 44-45 0.31910409714758003 1102 46-47 0.27433274398104857 1102 48-49 -0.1888184882429087 1102 50-51 0.06867966619842747 1102 52-53 0.1883693049088393 1102 54-55 0.1980362712141357 1102 56-57 0.11574537577278576 1102 58-59 -0.09439799183184405 1102 60-61 -0.10666158806916926 1102 62-63 0.07613014455983702 1102 64-65 -0.4066256230528751 1102 66-67 -0.09016371598710649 1102 68-69 0.1229746144321382 1102 70-71 0.3726801705054861 1102 72-73 0.2608522367537027 1102 74-75 0.011463717361415604 1102 76-77 0.1537842821146036 1102 78-79 0.08769315505995934 1102 80-81 0.394932074776964 1102 82-83 0.2743531547614708 1102 84-85 0.11988743832081994 1102 86-87 0.37525048957122564 1102 88-89 0.3094278948581213 1102 90-91 0.13013479198978928 1102 92-93 0.3408237448785556 1102 94-95 0.32204983305273416 1102 96-97 0.07433149546482198 1102 98-99 0.5717413751326532 1102 100 0.36685848164976775 1103 1 0.40377308345216534 1103 2 0.3889436882390278 1103 3 0.01918905228201595 1103 4 0.13859262767284264 1103 5 0.1081636104373942 1103 6 0.37905159137198297 1103 7 0.29548559396335605 1103 8 0.3661091430323111 1103 9 -0.09646358999728477 1103 10-11 0.19276767677554574 1103 12-13 0.28678502179131016 1103 14-15 -0.06781281411234374 1103 16-17 0.034211449812950434 1103 18-19 0.23519536952527886 1103 20-21 0.2913679154179647 1103 22-23 0.21589802788887624 1103 24-25 0.05167920589122588 1103 26-27 0.10706542871962199 1103 28-29 0.21178370992107887 1103 30-31 0.15450474020384064 1103 32-33 0.18140084225840525 1103 34-35 0.10543489117188187 1103 36-37 -0.04356686834703538 1103 38-39 -0.002788709087106156 1103 40-41 -0.45982734927012103 1103 42-43 -0.2756311008147563 1103 44-45 -0.46289335832316425 1103 46-47 -0.20398671010176628 1103 48-49 -0.31539146996856005 1103 50-51 -0.316700856586678 1103 52-53 -0.6266492007585143 1103 54-55 -0.6066074946891717 1103 56-57 -0.27815932004030586 1103 58-59 -0.8815943832267124 1103 60-61 -0.3517865013238506 1103 62-63 -0.5675316643691453 1103 64-65 -0.3648722110704554 1103 66-67 -1.1116476391885861 1103 68-69 -0.10528122641912319 1103 70-71 0.03255178743817311 1103 72-73 0.23818857725797926 1103 74-75 0.3042407240697216 1103 76-77 0.22744840425200508 1103 78-79 0.49479470490960153 1103 80-81 0.3305670504882734 1103 82-83 0.30439594911723944 1103 84-85 0.04067161597522784 1103 86-87 0.1673219677114055 1103 88-89 0.16370733043526542 1103 90-91 0.3455638921517128 1103 92-93 -0.008331934048115386 1103 94-95 0.4576443276166948 1103 96-97 0.21592876587888554 1103 98-99 0.05487808575028197 1103 100 0.1808418265491447 1104 1 0.1550219084823823 1104 2 0.2015796490286057 1104 3 0.15486337303757836 1104 4 0.06315509130472918 1104 5 0.11879205142033555 1104 6 0.2833821942993353 1104 7 0.18947646276959063 1104 8 0.3038044118206429 1104 9 -0.07108424260907498 1104 10-11 0.20048666876336796 1104 12-13 0.341776964855633 1104 14-15 0.37986298374809735 1104 16-17 0.010077570398415503 1104 18-19 0.24495768992276368 1104 20-21 0.3197551854595986 1104 22-23 0.32646156577885677 1104 24-25 0.18064724671302912 1104 26-27 0.31199023065329357 1104 28-29 0.24432253987497887 1104 30-31 0.1802925294702078 1104 32-33 -0.05692443413925474 1104 34-35 0.10437741836404513 1104 36-37 0.02944239712899588 1104 38-39 -0.008211474406472519 1104 40-41 0.15879760037402946 1104 42-43 -0.02727270145931726 1104 44-45 0.06604558800869853 1104 46-47 0.27811458348401885 1104 48-49 0.2437251765557633 1104 50-51 0.3084949717110703 1104 52-53 0.5920417709974473 1104 54-55 0.4785599105299312 1104 56-57 0.3766032241609416 1104 58-59 0.30956312986581125 1104 60-61 0.19667260972757816 1104 62-63 0.1331554132012407 1104 64-65 0.0535692194832329 1104 66-67 0.05815339926089891 1104 68-69 -0.24653049905687396 1104 70-71 -0.04317333677292012 1104 72-73 -0.35157799541110535 1104 74-75 -0.18111467631521805 1104 76-77 0.0792209301013429 1104 78-79 0.10545224038249756 1104 80-81 -0.35162040754851986 1104 82-83 -0.19515308628851358 1104 84-85 -0.4729378912088755 1104 86-87 -0.09228928514209045 1104 88-89 -0.04902486597492128 1104 90-91 -0.5203731742284852 1104 92-93 0.24282065536149133 1104 94-95 0.19098101800656408 1104 96-97 0.15793337623652448 1104 98-99 -0.017580622454367756 1104 100 -0.13889095175084165 1105 1 0.22105105044195028 1105 2 0.39515275294953156 1105 3 0.03461128614805631 1105 4 -0.017232191120278628 1105 5 -0.04545179433075219 1105 6 0.37827873310633464 1105 7 0.15494328324813722 1105 8 0.24022930721011448 1105 9 0.06112090044023688 1105 10-11 0.18549320293046634 1105 12-13 0.10012665134331655 1105 14-15 -0.13891795776791582 1105 16-17 -0.21748490858369962 1105 18-19 0.0640400119145923 1105 20-21 0.04487852584500729 1105 22-23 0.06345282659641072 1105 24-25 -0.10376355019995032 1105 26-27 0.29650084920692166 1105 28-29 0.11604534645938003 1105 30-31 0.31072472099154425 1105 32-33 -0.06023089296122919 1105 34-35 0.05510085920960961 1105 36-37 0.3957048109822807 1105 38-39 0.13648733667309187 1105 40-41 -0.024498557024905665 1105 42-43 -0.2507358951558629 1105 44-45 0.04313449950584669 1105 46-47 0.22362878199499647 1105 48-49 0.4570061372273173 1105 50-51 0.3461451830605142 1105 52-53 0.31947122540513817 1105 54-55 -0.12128088565939521 1105 56-57 -0.014400696339318841 1105 58-59 0.24351696076071505 1105 60-61 0.15918415626063975 1105 62-63 -0.01244477239464814 1105 64-65 0.11289392289164368 1105 66-67 0.14975092862208683 1105 68-69 0.2358549188956971 1105 70-71 0.6016702050436216 1105 72-73 0.19129529282867352 1105 74-75 0.22618422245414394 1105 76-77 0.3172178567015749 1105 78-79 0.3641467265670961 1105 80-81 0.2611407995058279 1105 82-83 0.3499443728356937 1105 84-85 0.43630955152670836 1105 86-87 0.4576481400980974 1105 88-89 0.5822384630874353 1105 90-91 0.14602673382493236 1105 92-93 0.47158248390665136 1105 94-95 -0.21247400266013372 1105 96-97 0.49182825064004376 1105 98-99 0.41062867521844737 1105 100 0.10459084410219788 1106 1 0.15050459066402055 1106 2 0.3121539568641438 1106 3 -0.04502955298462297 1106 4 0.026517730226764513 1106 5 0.1439780870815639 1106 6 0.3009848982397898 1106 7 0.17229517448473075 1106 8 0.3293022090638047 1106 9 0.2744507940175396 1106 10-11 0.19675735521936843 1106 12-13 0.3312835000234102 1106 14-15 0.0870006039106812 1106 16-17 -0.0762126602836517 1106 18-19 0.20941794462122232 1106 20-21 0.19323373518771803 1106 22-23 0.04905933528599604 1106 24-25 -0.08108842676723782 1106 26-27 0.18299833860450576 1106 28-29 0.05400284449829229 1106 30-31 0.20308552623004061 1106 32-33 -0.4838399940523104 1106 34-35 -5.099282008771411E-4 1106 36-37 0.05246330235562624 1106 38-39 0.1426327544566348 1106 40-41 0.38950439613149257 1106 42-43 0.4349385696894075 1106 44-45 0.43910809728091493 1106 46-47 0.5547965372185999 1106 48-49 0.4018011990800048 1106 50-51 0.3381886498312099 1106 52-53 0.26473296147517544 1106 54-55 0.4087855184112641 1106 56-57 0.37287283557700235 1106 58-59 -0.04507857007334337 1106 60-61 0.18197358865894486 1106 62-63 0.4370799539979302 1106 64-65 0.31050161452170855 1106 66-67 0.13989728604629548 1106 68-69 0.25841468465670303 1106 70-71 0.27515136398971407 1106 72-73 0.5410104642501743 1106 74-75 0.6120315140657517 1106 76-77 0.37177154835681137 1106 78-79 0.3415460612679446 1106 80-81 0.2932175731824671 1106 82-83 0.6095798845413505 1106 84-85 0.2117249440154545 1106 86-87 0.5244643522555386 1106 88-89 0.5801358073440923 1106 90-91 0.48671778920080655 1106 92-93 0.6054036753206837 1106 94-95 0.13537027706754046 1106 96-97 0.6907898218534783 1106 98-99 0.5190040616666494 1106 100 0.7293994552377789 1107 1 0.2957085673231319 1107 2 0.43029266184606385 1107 3 0.0747120239340262 1107 4 0.27490738818114835 1107 5 0.15347152832595867 1107 6 0.45867035970042735 1107 7 0.5026703446964973 1107 8 0.36576701204501916 1107 9 0.38447482642500574 1107 10-11 0.36026523298277624 1107 12-13 0.4966823824951234 1107 14-15 0.3577248202962551 1107 16-17 0.18839033544345085 1107 18-19 0.372585398850795 1107 20-21 0.3432740737757456 1107 22-23 0.31834182065525596 1107 24-25 0.343541375979207 1107 26-27 0.4523000328251925 1107 28-29 0.22874363171971623 1107 30-31 0.28488108428985726 1107 32-33 0.21705577871978932 1107 34-35 0.3895258002627955 1107 36-37 0.21552404074387255 1107 38-39 0.24041754702238194 1107 40-41 0.12433687360377377 1107 42-43 0.017843972499022698 1107 44-45 -0.1947359780690121 1107 46-47 0.0578656848151482 1107 48-49 0.034486340393122816 1107 50-51 -0.04641737662577583 1107 52-53 -0.1094693376109106 1107 54-55 -0.15409038813980658 1107 56-57 -0.17512901701000416 1107 58-59 -0.2836471691504663 1107 60-61 -0.3220944192124158 1107 62-63 -0.2181670504884039 1107 64-65 -0.2723013410802331 1107 66-67 -0.22618820614850677 1107 68-69 -0.46398112866741315 1107 70-71 -0.2637092096292726 1107 72-73 -0.6578769163881582 1107 74-75 -0.17789515080809082 1107 76-77 -0.3489445576248258 1107 78-79 -0.40613393634162165 1107 80-81 -0.13570860933577222 1107 82-83 -0.006478928693120167 1107 84-85 -0.07457765088694046 1107 86-87 -0.24022935193668715 1107 88-89 -0.2497335297797889 1107 90-91 -0.08718293091769169 1107 92-93 -0.11708071215172922 1107 94-95 -0.012614714416539385 1107 96-97 -0.21957954301652194 1107 98-99 -0.3998139963611571 1107 100 -0.5527358860608267 1108 1 0.25392587184025217 1108 2 0.3560177906072468 1108 3 0.2051325205784309 1108 4 0.21563617236344612 1108 5 0.3353472111504061 1108 6 0.46831820710919203 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0.4616226639689849 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 2.0 13 8.0 14 28.0 15 61.0 16 207.0 17 787.0 18 2560.0 19 5835.0 20 10226.0 21 15636.0 22 20971.0 23 23613.0 24 19682.0 25 16669.0 26 17352.0 27 20281.0 28 26167.0 29 32821.0 30 32024.0 31 26301.0 32 27933.0 33 37112.0 34 56992.0 35 112837.0 36 163297.0 37 24409.0 38 175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.541161611428386 21.075419820032845 14.224066461833035 18.159352106705732 2 13.381357373212788 25.87807907683174 35.415832082056845 25.32473146789863 3 27.736254425515178 28.723592805052544 33.73492587036933 9.805226899062951 4 10.359271859559328 21.382244912368677 34.70093820201243 33.557545026059564 5 7.436949419751645 44.103198175024175 34.06799274275248 14.3918596624717 6 21.072645662370224 36.659293788770654 14.200883304394187 28.067177244464936 7 16.228705974575416 38.63948793470649 19.324210993495434 25.807595097222663 8 23.13767588636642 23.975590250509736 22.58017104116083 30.306562821963013 9 18.021138785420433 22.234774526826946 23.146897987708666 36.59718870004395 10-11 23.826931985939108 23.987022708818607 24.426731836511493 27.759313468730795 12-13 32.78452303072396 22.275665503338686 23.059038654958457 21.880772810978897 14-15 25.245285574513645 25.88405834691428 28.38583431678115 20.48482176179093 16-17 18.358276271499054 31.20597881219164 27.75431422050076 22.68143069580855 18-19 18.258332859019184 32.3431575200887 27.24052194249295 22.157987678399156 20-21 21.907286314133138 24.463173608689512 24.724922404774734 28.904617672402615 22-23 17.730460174657576 29.17578367878914 27.522581491138514 25.57117465541477 24-25 24.91743349289467 29.255345208692972 25.550227238013445 20.276994060398913 26-27 21.847747773711056 23.182584512521977 35.80729992218796 19.16236779157901 28-29 15.08397558148164 31.696594217569203 32.27888639682758 20.94054380412158 30-31 19.234795589815047 25.630111335339624 27.485484185885994 27.64960888895934 32-33 20.880948212447198 26.4353630042386 33.94646383646779 18.73722494684641 34-35 22.152848124521782 27.860985067357557 30.881231961013455 19.104934847107206 36-37 17.880954542376156 25.356094566612917 34.90994788086809 21.853003010142842 38-39 18.41545180892162 26.008570889236964 34.19645031463579 21.379526987205626 40-41 16.8832806298196 28.58828793194006 33.18498537054209 21.343446067698256 42-43 18.593195451722462 27.561432151209626 29.866407676335093 23.97896472073282 44-45 18.36747486217063 24.480672186566068 31.435759841146787 25.716093110116518 46-47 23.899868152769873 26.14544982960727 30.42580171041161 19.528880307211253 48-49 20.607667057647028 24.234850471626064 31.09972592949097 24.05775654123594 50-51 12.574875753440645 26.405473909303257 37.39630509681073 23.62334524044537 52-53 17.583652684386056 29.218838145753768 30.869304906226425 22.32820426363375 54-55 15.173066199027929 24.696498329209792 30.278695587644076 29.8517398841182 56-57 22.382400195969655 24.838505155010555 25.915761868051902 26.863332780967887 58-59 22.406912546317677 28.150916915291337 28.331396450497092 21.110774087893894 60-61 18.78378602311445 33.79729863115948 27.84505062454835 19.573864721177724 62-63 13.156690776339138 36.0849676828561 27.41982315090532 23.33851838989944 64-65 12.842567288620032 39.89786294642632 24.26381851075588 22.99575125419777 66-67 16.498767232447655 33.27420468120242 22.205738889601378 28.02128919674855 68-69 15.320890984388463 31.549885154198858 25.234374774847474 27.894849086565205 70-71 20.08840407941237 30.204510985665863 23.685592728818506 26.021492206103268 72-73 18.27639820916255 25.34803026613274 30.23861021330678 26.136961311397926 74-75 15.750723359616359 31.22471123253562 25.478469244247705 27.546096163600314 76-77 17.374758277639927 25.930577049992937 26.159258650850603 30.535406021516533 78-79 18.17567440995883 25.67332923137222 27.796310252357063 28.35468610631188 80-81 20.14264346482835 23.43090747700182 26.69654796762963 29.7299010905402 82-83 17.76431401453827 22.6635051079923 30.143819217636715 29.428361659832714 84-85 17.90787768226583 24.674792266918548 28.005222056271116 29.412107994544506 86-87 18.043630657457047 25.299556402196306 26.139632801597152 30.517180138749495 88-89 17.293843605518685 24.576584417614892 24.32700572575596 33.80256625111046 90-91 19.204333631397787 20.80862172450342 25.81632072215661 34.170723921942184 92-93 20.607813280313735 23.037653954886743 26.680685440196932 29.673847324602587 94-95 19.27355865326833 21.90982495024621 27.556393632091446 31.260222764394012 96-97 15.534654228663944 22.909120777694596 30.111960757894856 31.4442642357466 98-99 18.84702981675338 24.126042664118952 24.901812868404384 32.12511465072328 100 18.612486040563418 19.465110414640296 25.49472243236428 36.427681112432005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3976.0 1 2258.5 2 517.0 3 436.0 4 322.5 5 335.5 6 576.5 7 1006.5 8 1277.0 9 1183.0 10 806.5 11 450.5 12 371.0 13 371.0 14 363.5 15 363.5 16 393.5 17 430.5 18 482.0 19 554.5 20 645.5 21 819.5 22 1016.0 23 1288.5 24 1665.5 25 2130.0 26 2779.5 27 3568.0 28 4454.5 29 5465.0 30 6625.5 31 7775.5 32 8707.0 33 9595.5 34 10289.5 35 10476.0 36 10484.5 37 10286.0 38 10099.0 39 10221.5 40 10264.5 41 10434.5 42 11047.0 43 11844.0 44 12616.0 45 14033.5 46 21311.5 47 51396.0 48 95443.0 49 95512.0 50 73904.5 51 64500.5 52 45284.0 53 22642.0 54 7848.0 55 3488.0 56 2231.0 57 1705.5 58 1348.0 59 1025.5 60 784.0 61 621.5 62 488.0 63 369.0 64 248.5 65 150.5 66 100.0 67 77.5 68 62.0 69 53.5 70 54.5 71 50.5 72 35.5 73 34.0 74 32.0 75 21.0 76 13.0 77 7.0 78 6.0 79 7.0 80 4.5 81 1.5 82 3.5 83 4.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05994348597308019 2 0.004178752627931071 3 0.0 4 0.0 5 0.00864569509227118 6 2.8818983640903936E-4 7 0.001873233936658756 8 2.8818983640903936E-4 9 2.8818983640903936E-4 10-11 7.925220501248582E-4 12-13 1.4409491820451968E-4 14-15 0.0 16-17 4.3228475461355904E-4 18-19 3.602372955112992E-4 20-21 1.4409491820451968E-4 22-23 7.204745910225984E-5 24-25 1.4409491820451968E-4 26-27 0.0010086644274316379 28-29 7.925220501248582E-4 30-31 3.602372955112992E-4 32-33 0.0010807118865338975 34-35 5.763796728180787E-4 36-37 0.0 38-39 0.0014409491820451968 40-41 0.0015129966411474567 42-43 0.0014409491820451968 44-45 0.001873233936658756 46-47 2.8818983640903936E-4 48-49 7.204745910225984E-4 50-51 5.763796728180787E-4 52-53 2.1614237730677952E-4 54-55 5.763796728180787E-4 56-57 2.8818983640903936E-4 58-59 3.602372955112992E-4 60-61 7.925220501248582E-4 62-63 7.925220501248582E-4 64-65 0.0025216610685790944 66-67 0.0028818983640903936 68-69 0.001873233936658756 70-71 0.0020893763139655354 72-73 0.0014409491820451968 74-75 4.3228475461355904E-4 76-77 0.0012968542638406772 78-79 0.0014409491820451968 80-81 0.002737803445885874 82-83 0.0036744204142152514 84-85 0.0010807118865338975 86-87 3.602372955112992E-4 88-89 0.0025216610685790944 90-91 0.009438217142396038 92-93 0.022406759780802813 94-95 0.010086644274316377 96-97 0.005547654350874008 98-99 0.0045389899234423705 100 0.0017291390184542362 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 693987.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.98123574733258 #Duplication Level Percentage of deduplicated Percentage of total 1 81.44853979808163 17.08891014779551 2 8.531872607659901 3.5801846109504307 3 3.119033980811557 1.9632356176604544 4 1.6173566643297408 1.3573656584728695 5 1.0034060899511714 1.0526349861787365 6 0.6901438563825162 0.8688042570200888 7 0.5379141138154767 0.7900271983646006 8 0.39822280204128596 0.6684165191673261 9 0.2828148088086522 0.5340423758806011 >10 1.9053446992632654 7.685076570462429 >50 0.2045346202046206 3.044870161521157 >100 0.20041647348909133 8.686462738505305 >500 0.021277091363567917 3.067767170397522 >1k 0.024708880293175646 12.250043755200469 >5k 0.008236293431058547 12.772642781693106 >10k+ 0.0061772200732939115 24.589515450729387 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 37170 5.356008109661996 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 30982 4.464348755812429 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 22391 3.2264293135174005 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 21444 3.08997142597772 RNA PCR Primer, Index 26 (100% over 22bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14699 2.118051202688235 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 12234 1.7628572293140938 RNA PCR Primer, Index 26 (100% over 24bp) ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10768 1.5516140792262678 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10678 1.5386455365878613 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10387 1.496713915390346 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9966 1.436049954826243 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9417 1.3569418447319619 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9182 1.3230795389538996 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8901 1.2825888669384298 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7973 1.1488687828446353 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7057 1.0168778377692953 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6639 0.9566461619598062 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6520 0.9394988666934683 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6201 0.8935325877862265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5890 0.848719068224621 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5691 0.8200441795019214 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5258 0.7576510799193645 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4868 0.7014540618196018 No Hit CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT 4818 0.6942493159093758 RNA PCR Primer, Index 26 (100% over 27bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4660 0.6714823188330618 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3939 0.567589882807603 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3610 0.520182654718316 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3518 0.5069259222435002 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3388 0.48819358287691267 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3349 0.48257388106693644 No Hit CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG 3325 0.4791156030300279 RNA PCR Primer, Index 26 (96% over 27bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3231 0.4655706807188031 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3197 0.4606714534998494 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3098 0.446406056597602 No Hit TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 3006 0.4331493241227862 TruSeq Adapter, Index 1 (96% over 28bp) CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2859 0.4119673711467218 No Hit TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC 2284 0.32911279317912295 RNA PCR Primer, Index 26 (95% over 22bp) AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2024 0.29164811444594785 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1960 0.2824260396808586 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1923 0.27709452770729137 No Hit TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT 1858 0.2677283580239976 RNA PCR Primer, Index 26 (100% over 21bp) CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1835 0.2644141749052936 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 1660 0.23919756421950264 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1647 0.23732433028284391 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1626 0.234298337000549 No Hit CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC 1581 0.22781406568134563 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1499 0.215998282388575 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1479 0.2131163840244846 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1424 0.20519116352323602 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1412 0.2034620245047818 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1361 0.19611318367635128 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1353 0.19496042433071514 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1311 0.18890843776612531 No Hit ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC 1287 0.18545015972921683 RNA PCR Primer, Index 26 (96% over 28bp) CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1275 0.1837210207107626 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1161 0.16729420003544737 No Hit CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC 1147 0.1652768711805841 RNA PCR Primer, Index 26 (95% over 22bp) ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1093 0.15749574559754 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 971 0.13991616557658862 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 935 0.1347287485212259 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 918 0.13227913491174906 No Hit CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA 863 0.12435391441050049 RNA PCR Primer, Index 1 (96% over 28bp) CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 851 0.12262477539204625 No Hit TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 841 0.12118382621000105 RNA PCR Primer, Index 26 (100% over 29bp) CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 828 0.1193105922733423 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 817 0.11772554817309258 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC 810 0.11671688374566094 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 806 0.11614050407284286 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 758 0.10922394799902592 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 722 0.1040365309436632 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 694 0.10000187323393665 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.4409491820451968E-4 0.0 10-11 0.0 0.0 0.0 3.602372955112992E-4 0.0 12-13 0.0 0.0 0.0 4.3228475461355904E-4 0.0 14-15 0.0 0.0 0.0 5.763796728180787E-4 0.0 16-17 0.0 0.0 0.0 8.645695092271181E-4 0.0 18-19 0.0 0.0 0.0 0.002233471232170055 0.0 20-21 0.0 0.0 0.0 0.012536257883793213 0.0 22-23 0.0 0.0 0.0 0.04827179759851409 0.0 24-25 0.0 0.0 0.0 0.10655819201224231 0.0 26-27 0.0 0.0 0.0 0.1985627972858281 0.0 28-29 0.0 0.0 0.0 0.4832223081988568 0.0 30-31 0.0 0.0 0.0 0.9763871657538253 0.0 32-33 0.0 0.0 0.0 1.7935494468916564 0.0 34-35 0.0 0.0 0.0 2.936078053335293 0.0 36-37 0.0 0.0 0.0 4.227744900120608 0.0 38-39 0.0 0.0 0.0 5.6926138385877545 0.0 40-41 0.0 0.0 0.0 7.078086477124211 0.0 42-43 0.0 0.0 0.0 8.300155478416743 0.0 44-45 0.0 0.0 0.0 9.580006541909288 0.0 46-47 0.0 0.0 0.0 11.042714056603366 0.0 48-49 0.0 0.0 0.0 12.155342967519564 0.0 50-51 0.0 0.0 0.0 13.155145557481625 0.0 52-53 0.0 0.0 0.0 14.02346153458206 0.0 54-55 0.0 0.0 0.0 14.99235576458925 0.0 56-57 0.0 0.0 0.0 16.324441235930934 0.0 58-59 0.0 0.0 0.0 17.21674901691242 0.0 60-61 0.0 0.0 0.0 18.066548797023575 0.0 62-63 0.0 0.0 0.0 18.85251452837013 0.0 64-65 0.0 0.0 0.0 19.624791242487248 0.0 66-67 0.0 0.0 0.0 20.56976571607249 0.0 68-69 0.0 0.0 0.0 21.64348899907347 0.0 70-71 0.0 0.0 0.0 22.645020728053986 0.0 72-73 0.0 0.0 0.0 23.39532296714492 0.0 74-75 0.0 0.0 0.0 24.31832296570397 0.0 76-77 0.0 0.0 0.0 25.028206580238535 0.0 78-79 0.0 0.0 0.0 26.268143351388424 0.0 80-81 0.0 0.0 0.0 27.336823312252246 0.0 82-83 0.0 0.0 0.0 28.124229992780844 0.0 84-85 0.0 0.0 0.0 29.011278309247867 0.0 86-87 0.0 0.0 0.0 29.789607009929583 0.0 88 0.0 0.0 0.0 30.485009085184593 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGAAC 20 0.0021626244 70.49995 94 CTTTACA 340 0.0 66.37681 1 ATAACGC 30 1.370785E-4 62.666622 3 CTTAATA 65 7.3305273E-10 57.866962 1 GTACCCA 300 0.0 56.420284 1 GCGCTTC 25 0.005235184 56.399956 94 TATAACG 35 2.9367095E-4 53.714245 2 ACCCATG 345 0.0 47.68112 3 CTATACA 300 0.0 45.449673 1 CGGAGAT 65 2.7764436E-6 43.40022 1 TACAGGG 130 0.0 43.384583 2 CTATGAT 45 0.0010136814 41.77775 3 GTACAGG 150 0.0 40.747982 1 TACCCAT 405 0.0 40.61725 2 GCTTCCG 60 8.8279965E-5 39.160995 9 ATGGTAC 50 0.0017027741 37.59455 8 GAGATGT 180 0.0 36.568703 1 TAGGCAT 470 0.0 36.494713 18-19 TAACGCA 80 1.1579945E-5 35.249973 4 AGACTAG 480 0.0 35.244896 14-15 >>END_MODULE