FastQCFastQC Report
Mon 6 Jun 2016
ERR522842_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522842_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7923900
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT399530.5042087860775628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280490.353979732202577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223690.282297858377819No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124780.1574729615467131No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT116730.14731382273880286No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC90280.11393379522709776No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC80960.10217191029669734No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG275650.028.1308631
TACATGG282950.026.8037472
ACATGGG288300.025.4597363
CATGGGG198100.022.7135264
ATGGGGG115350.021.3985825
GAGTACT240950.020.902412-13
AGAGTAC360250.019.6573610-11
TACCTGG58200.018.9044342
GTACTTT264550.018.85251814-15
AGTACTT254900.018.23787312-13
GAGTACA214550.016.9422631
AGTACAT205850.016.2401312
GTACCTG71650.016.1450481
CATGGGA104000.015.8683174
ACTTTTT323200.015.60433216-17
GTGTAGC41200.015.5225141
TGGGGGG117900.015.3814626
GTATCAA716950.015.315051
GTATAGG22050.014.9282831
TACTTTT307800.014.89835514-15
Produced by FastQC (version 0.11.5)