FastQCFastQC Report
Mon 6 Jun 2016
ERR522842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7923900
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT400210.5050669493557466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255380.3222907911508222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215420.2718610785093199No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120960.15265210313103397No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT115070.14521889473617788No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC94050.11869155340173398No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC84990.10725778972475673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG273900.026.7875461
TACATGG280650.025.5745742
ACATGGG285400.024.4359593
CATGGGG198100.022.2044934
GAGTACT243350.020.83544212-13
ATGGGGG117550.020.308715
AGAGTAC364800.019.89135410-11
GTACTTT259950.019.30214914-15
TACCTGG55850.018.6837582
AGTACTT255100.018.52121712-13
GAGTACA213700.017.9811691
TATCACG6650.017.670662
AGTACAT209100.016.8818632
ACTTTTT319000.015.74093616-17
TACTTTT304250.015.42570614-15
GTATCAA694100.015.3952481
TATAACG4903.45608E-1115.348232
GTACCTG69700.015.3176971
TGGGGGG112500.014.9962656
CATGGGA101200.014.9528794
Produced by FastQC (version 0.11.5)