FastQCFastQC Report
Mon 6 Jun 2016
ERR522841_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522841_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305910
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT292790.6799724100132144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150200.3488228969021647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115990.26937395347324955No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT76490.1776395698005764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74720.17352894045625664No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC59640.1385073073984361No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC55220.12824234598493697No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA45040.10460042128144806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG43550.040.3732762
GTACCTG54350.032.9584161
ACCTGGG52500.032.507093
GTACATG166950.030.1608751
TACATGG167450.029.3106772
ATGGGGG87150.029.129425
CATGGGG126050.028.0080134
ACATGGG171500.027.9070823
TATCACG3650.025.7601072
TGGGGGG92300.025.7031526
CCTGGGG50400.024.4374564
TATAACG3850.024.4219192
GAGTACT129400.020.02225512-13
AGAGTAC197450.019.52126910-11
GAGTACA123000.018.6150741
ATAACGC4800.018.6098633
CTGGGGG34800.017.9670945
AGTACTT135000.017.62574412-13
GGGTTAG15300.017.5155741
AGTACAT120450.017.4075872
Produced by FastQC (version 0.11.5)