##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522841_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4305910 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.830712903892557 34.0 31.0 34.0 30.0 34.0 2 32.13096000613111 34.0 31.0 34.0 29.0 35.0 3 31.96479280802432 34.0 31.0 34.0 28.0 35.0 4 34.589058294297836 35.0 35.0 37.0 32.0 37.0 5 34.59838245574106 37.0 34.0 37.0 32.0 37.0 6 34.76811219927959 37.0 35.0 37.0 32.0 37.0 7 34.61812160495691 37.0 35.0 37.0 32.0 37.0 8 34.75746729495043 37.0 35.0 37.0 32.0 37.0 9 36.00596761195659 38.0 35.0 39.0 32.0 39.0 10-11 36.09373442547568 38.0 35.0 39.0 32.0 39.0 12-13 36.1297113734379 38.0 35.0 39.0 32.0 39.0 14-15 37.20364104219549 39.0 36.5 41.0 32.0 41.0 16-17 37.08255188798651 39.0 36.5 41.0 32.0 41.0 18-19 37.10963849685665 39.0 36.5 41.0 32.0 41.0 20-21 37.1299135374404 39.0 36.0 41.0 32.0 41.0 22-23 37.03101574347815 39.0 36.0 41.0 31.5 41.0 24-25 37.02906992946903 39.0 36.5 41.0 31.5 41.0 26-27 36.84949766715979 39.0 36.5 41.0 31.5 41.0 28-29 36.641013746223216 38.0 35.0 41.0 30.5 41.0 30-31 36.54586324377425 38.0 36.0 41.0 30.5 41.0 32-33 36.3596881959911 38.0 35.0 41.0 30.0 41.0 34-35 36.25206495258842 38.0 35.0 40.5 30.0 41.0 36-37 36.132456902257594 38.0 35.5 40.0 30.0 41.0 38-39 35.889218887529 38.0 35.0 40.0 29.0 41.0 40-41 35.83642911719009 38.0 35.0 40.0 29.0 41.0 42-43 35.791564152525254 38.0 35.0 40.0 29.0 41.0 44-45 35.69310250330359 38.0 35.0 40.0 28.0 41.0 46-47 35.40594287850884 38.0 34.5 40.0 27.0 41.0 48-49 35.51262265119336 37.5 35.0 40.0 27.0 41.0 50-51 34.502908096081896 37.5 33.5 39.0 25.5 40.0 52-53 34.67568597578677 37.0 34.5 39.5 26.0 40.0 54-55 35.256949982698195 37.0 34.5 40.0 27.0 41.0 56-57 35.39910913140312 37.0 35.0 40.0 27.5 41.0 58-59 35.381884084897266 38.0 35.0 40.0 26.5 41.0 60-61 35.10991172597662 37.0 35.0 40.0 26.0 41.0 62-63 34.88936392075078 37.0 34.0 40.0 26.0 41.0 64-65 34.65829836201871 36.5 34.0 39.5 26.0 41.0 66-67 34.41404627593238 37.0 34.0 39.0 25.5 41.0 68-69 34.06689050630412 36.0 34.0 39.0 25.5 41.0 70-71 33.65820000882508 36.0 33.5 38.0 24.0 40.0 72-73 33.39748659400684 35.5 33.5 37.5 23.5 40.0 74-75 33.14297163665752 35.0 33.0 37.0 23.0 39.0 76-77 32.797800697181316 35.0 33.0 37.0 22.5 39.0 78-79 32.49261665942855 35.0 32.5 37.0 22.0 38.5 80-81 32.27517969488447 35.0 32.0 36.0 20.5 38.0 82-83 32.04918077711796 35.0 32.0 36.0 20.0 37.5 84-85 31.753723370901852 35.0 32.0 35.5 19.5 37.0 86-87 31.646061111356254 35.0 32.0 36.0 19.5 37.0 88-89 31.347043249858913 35.0 31.0 35.0 19.0 37.0 90-91 31.240874170616664 35.0 31.0 35.5 18.0 36.5 92-93 30.993085898218958 35.0 31.0 35.0 18.0 36.0 94-95 30.89354991163308 34.5 31.0 35.0 18.5 36.0 96-97 30.626544563170157 34.0 30.0 35.0 18.0 36.0 98-99 30.562513731127684 34.0 30.0 35.0 18.0 36.5 100 29.319918902160055 34.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.01391227840595377 1101 2 0.02363854282302924 1101 3 -0.008429513977535663 1101 4 0.04291440498610655 1101 5 0.04375016276263466 1101 6 -0.03158172286644145 1101 7 0.0961727950544713 1101 8 0.0401400363616915 1101 9 -0.04291130386031483 1101 10-11 -0.015167404208547453 1101 12-13 -0.019405914616683617 1101 14-15 0.021619561567739254 1101 16-17 -0.12361060030978166 1101 18-19 -0.03828674004873989 1101 20-21 0.017641767470806258 1101 22-23 -0.040220288004121585 1101 24-25 -0.1306735715445626 1101 26-27 -0.1556274874618353 1101 28-29 -0.17551257106193674 1101 30-31 -0.10590348238309844 1101 32-33 -0.20852248554283648 1101 34-35 -0.21048499173466695 1101 36-37 -0.1184847143406671 1101 38-39 -0.2546134680606045 1101 40-41 -0.28410008013535304 1101 42-43 -0.16093453217149545 1101 44-45 -0.27625414849597263 1101 46-47 -0.22009125462061974 1101 48-49 -0.31940699904093606 1101 50-51 -0.47336874771028903 1101 52-53 -0.277021823741741 1101 54-55 -0.24566351489423255 1101 56-57 -0.21868290429186743 1101 58-59 -0.15116761959736635 1101 60-61 -0.2131778286598376 1101 62-63 -0.22578042028186474 1101 64-65 -0.1956956780340917 1101 66-67 -0.4730087869656572 1101 68-69 -0.2602134901942108 1101 70-71 -0.18589065040068675 1101 72-73 -0.26942907583767806 1101 74-75 -0.3368746757129628 1101 76-77 -0.2852401981098964 1101 78-79 -0.5316539672438658 1101 80-81 -0.24182053598087094 1101 82-83 -0.3979068569067721 1101 84-85 -0.6726218948889517 1101 86-87 -0.9244762953781169 1101 88-89 -0.5292028702187643 1101 90-91 -0.7223271627987344 1101 92-93 -0.5316672143843348 1101 94-95 -0.6965085081042304 1101 96-97 -0.6652628090246324 1101 98-99 -0.6516150193894745 1101 100 -0.7141524311722627 1102 1 -0.0539570109920966 1102 2 -0.07614685808164268 1102 3 0.05194598224314362 1102 4 -0.060064292509302675 1102 5 -0.03305149249115402 1102 6 0.003501208075363138 1102 7 0.1496091186909254 1102 8 0.007663647248513428 1102 9 -0.15669128114711128 1102 10-11 -0.06135059101648466 1102 12-13 -0.11027816601529139 1102 14-15 -0.12888271415544494 1102 16-17 -0.15674716871995287 1102 18-19 -0.12199420900353886 1102 20-21 -0.11205517932241094 1102 22-23 -0.2011954159823901 1102 24-25 -0.1481099448694252 1102 26-27 -0.23381018298439926 1102 28-29 -0.4459202615208895 1102 30-31 -0.2753301009173299 1102 32-33 -0.31683175178329037 1102 34-35 -0.3335558262322067 1102 36-37 -0.2805296010508158 1102 38-39 -0.44485652543257714 1102 40-41 -0.8123610028222359 1102 42-43 -0.28038053326662293 1102 44-45 -0.35220194464309884 1102 46-47 -0.39144038291859573 1102 48-49 -0.5925076320024161 1102 50-51 -0.42853668315175497 1102 52-53 -0.6877415259005701 1102 54-55 -0.3411370760255821 1102 56-57 -0.16853954565768703 1102 58-59 -0.1648457239869927 1102 60-61 -0.7409648160943192 1102 62-63 -0.3049216879789114 1102 64-65 -0.30838460988906746 1102 66-67 -0.2711690853806985 1102 68-69 -0.5764070351686499 1102 70-71 -0.2622642971862206 1102 72-73 -0.49513898290694414 1102 74-75 -0.3127387947173901 1102 76-77 -0.4700713890389352 1102 78-79 -0.42471448556509017 1102 80-81 -0.36675322772723007 1102 82-83 -0.3486070931074927 1102 84-85 -0.36800857712465174 1102 86-87 -0.49272789973919373 1102 88-89 -0.3452579350041063 1102 90-91 -0.3731472518681187 1102 92-93 -0.48868995038778706 1102 94-95 -0.6035035626497951 1102 96-97 -0.47206043380216656 1102 98-99 -0.636088033890033 1102 100 -0.9325549640679434 1103 1 -0.05684445673736249 1103 2 -0.032574161821436576 1103 3 -0.09669352443042811 1103 4 -0.09941564596473995 1103 5 -0.022760188210575905 1103 6 -0.0704563981181181 1103 7 0.07922102519927421 1103 8 -0.07891767155444995 1103 9 -0.1362251018498668 1103 10-11 -0.11299730917964013 1103 12-13 -0.02022125990924195 1103 14-15 -0.07291334235040381 1103 16-17 -0.06795360701496378 1103 18-19 -0.24334443396399053 1103 20-21 -0.2374884221710971 1103 22-23 -0.26746443326901215 1103 24-25 -0.27407963120725043 1103 26-27 -0.4022025224949175 1103 28-29 -0.3105874901759691 1103 30-31 -0.24288560943777782 1103 32-33 -0.36239790819436735 1103 34-35 -0.3957796216976206 1103 36-37 -0.5177473621786248 1103 38-39 -0.40051765510575876 1103 40-41 -0.3984874655083033 1103 42-43 -0.23210079301857434 1103 44-45 -0.3111301315944388 1103 46-47 -0.43480420160381783 1103 48-49 -0.2864882564351561 1103 50-51 -0.42861123263566725 1103 52-53 -0.27964348828974295 1103 54-55 -0.24539729230249208 1103 56-57 -0.2426474672441401 1103 58-59 -0.15017129235729954 1103 60-61 -0.23748870746617 1103 62-63 -0.27436326368739117 1103 64-65 -0.23927732518806266 1103 66-67 -0.17456729066703502 1103 68-69 -0.18690900690693013 1103 70-71 -0.17282185955363616 1103 72-73 -0.23248672775029888 1103 74-75 -0.2517052712568102 1103 76-77 -0.4052713788904754 1103 78-79 -0.562355423623508 1103 80-81 -0.46686901402357606 1103 82-83 -0.4148258894131054 1103 84-85 -0.4896532638578144 1103 86-87 -0.5522892881112469 1103 88-89 -0.4683387853964973 1103 90-91 -0.29819895428117604 1103 92-93 -0.4963697636561015 1103 94-95 -0.5159237325774377 1103 96-97 -0.5442340656323275 1103 98-99 -0.5150341822275166 1103 100 -0.555292695681807 1104 1 0.14275519566683315 1104 2 0.18544477407701976 1104 3 0.21992817330505332 1104 4 0.14355066572922226 1104 5 0.1125477532771555 1104 6 0.14670996071377118 1104 7 0.23762776796399265 1104 8 0.09136396595026497 1104 9 0.08665313476488734 1104 10-11 0.026162655643211963 1104 12-13 0.06058267253506244 1104 14-15 0.06219764983332965 1104 16-17 0.09821920753208957 1104 18-19 0.04799053319024438 1104 20-21 0.04585038004309183 1104 22-23 0.09911998560527735 1104 24-25 0.13426180975644542 1104 26-27 0.08459329095209966 1104 28-29 -0.057238917049325266 1104 30-31 0.02839346958180755 1104 32-33 0.07778839680008076 1104 34-35 0.0036955919193033537 1104 36-37 -0.0898329532376394 1104 38-39 -0.10877640751890993 1104 40-41 0.08835059692810177 1104 42-43 0.04677921845970445 1104 44-45 0.048283702969072806 1104 46-47 -0.07280221772814599 1104 48-49 0.06274275976294774 1104 50-51 0.03544456247580996 1104 52-53 -0.01932203408475175 1104 54-55 0.11078082642104903 1104 56-57 -0.42581669935528055 1104 58-59 0.1841395381841835 1104 60-61 0.18284225435628798 1104 62-63 0.1828195748601189 1104 64-65 0.09425977180239187 1104 66-67 0.0738918826005559 1104 68-69 0.029034335023503388 1104 70-71 0.020729257025969616 1104 72-73 -0.013846583821880643 1104 74-75 -0.03906651362878932 1104 76-77 -0.06035990864651808 1104 78-79 0.021628665934834146 1104 80-81 -0.05771808590287719 1104 82-83 0.1193749139730258 1104 84-85 -0.13924658326319062 1104 86-87 -0.09294934433343016 1104 88-89 -0.12069597743297322 1104 90-91 -0.3670738251274379 1104 92-93 -0.4595316606235471 1104 94-95 -0.14358681077674973 1104 96-97 -0.16309871204917314 1104 98-99 -0.20671544030905054 1104 100 -0.21040825087238346 1105 1 0.08536620926105343 1105 2 -0.02470780805540329 1105 3 0.05555828434761523 1105 4 0.1174459064774922 1105 5 0.01072754656194519 1105 6 8.260374354378541E-4 1105 7 0.16596146669597545 1105 8 0.06410478258612073 1105 9 -0.03177919147415054 1105 10-11 -0.005896991089677783 1105 12-13 -0.0011385764237843432 1105 14-15 -0.015212845651873863 1105 16-17 -0.012559996587931721 1105 18-19 -0.07140811990244345 1105 20-21 0.007230591550964505 1105 22-23 -0.08069954531556078 1105 24-25 -0.060251208091848696 1105 26-27 -0.1732266650053873 1105 28-29 -0.1613144658077985 1105 30-31 -0.005808973706855625 1105 32-33 -0.019977999672441626 1105 34-35 -0.1678110714688188 1105 36-37 -0.09949332485038553 1105 38-39 0.08487164683757698 1105 40-41 -0.15815620551235554 1105 42-43 -0.166603230863835 1105 44-45 -0.3168156255935273 1105 46-47 -0.15296253117066527 1105 48-49 -0.13287584006342712 1105 50-51 -0.17548367098935813 1105 52-53 -0.02405554954574285 1105 54-55 0.029763776750165505 1105 56-57 -3.4817408413800877E-4 1105 58-59 0.0856618020860509 1105 60-61 0.08138204835488239 1105 62-63 0.09041657197848707 1105 64-65 0.12327909706481677 1105 66-67 -0.04862131114894197 1105 68-69 -0.0237942271150402 1105 70-71 -0.10700961055958658 1105 72-73 -0.07338795560102795 1105 74-75 -0.09765479749579953 1105 76-77 -0.011429591945550044 1105 78-79 -0.11240035011689997 1105 80-81 0.08970033788322951 1105 82-83 0.11365169348376014 1105 84-85 0.049806428587313434 1105 86-87 0.09275215849790897 1105 88-89 0.11859986035969072 1105 90-91 -0.07415911542932108 1105 92-93 -0.04683938020689027 1105 94-95 0.04976167269525433 1105 96-97 0.0037463085560673903 1105 98-99 0.04686289783935749 1105 100 0.04576138556600284 1106 1 0.07697078486865294 1106 2 -0.027533070662492776 1106 3 0.05489398072141327 1106 4 0.10215869376894915 1106 5 0.0499413243496889 1106 6 0.0018882989451824983 1106 7 0.17937824329954566 1106 8 -0.016155865071610265 1106 9 -0.0043660681148693925 1106 10-11 0.010521212021508575 1106 12-13 0.043088040500038005 1106 14-15 0.020133385368581003 1106 16-17 -0.033263653322585185 1106 18-19 -0.047127240637998113 1106 20-21 -0.0695090198725552 1106 22-23 -0.05551253433851855 1106 24-25 -0.011230452931009438 1106 26-27 -0.008765092884459591 1106 28-29 -0.004743067072674023 1106 30-31 -0.10019153597786357 1106 32-33 -0.09592439113598772 1106 34-35 -0.09689028875398975 1106 36-37 -0.1218709642938407 1106 38-39 -0.06634270075866056 1106 40-41 -0.11758345717105811 1106 42-43 0.037440791950075436 1106 44-45 -0.02230218898146319 1106 46-47 -0.08083533220789008 1106 48-49 -0.04460436036895743 1106 50-51 -0.09942524745762427 1106 52-53 -0.1073767895829647 1106 54-55 -0.11089938044859338 1106 56-57 -0.07915498715488667 1106 58-59 0.12773773247293718 1106 60-61 0.10201589787379817 1106 62-63 0.05790990553126818 1106 64-65 0.06958548661008734 1106 66-67 0.060978628237954524 1106 68-69 0.12096877029329534 1106 70-71 0.030666138328534487 1106 72-73 0.06261937245401583 1106 74-75 0.154752742705071 1106 76-77 0.06071352646763728 1106 78-79 0.04194229718270748 1106 80-81 0.08336218321711186 1106 82-83 0.16474104076075236 1106 84-85 0.07171601916232007 1106 86-87 0.12989416595479497 1106 88-89 0.07822560855257876 1106 90-91 0.1601664262647411 1106 92-93 -0.024182483523556897 1106 94-95 0.11894025459288571 1106 96-97 0.12656699680413652 1106 98-99 0.056661787791117746 1106 100 0.039881461355072645 1107 1 -0.026398192271269494 1107 2 -0.03770582408654377 1107 3 0.08849173999957216 1107 4 0.19202626565708414 1107 5 0.0931022100770349 1107 6 0.08555654750912822 1107 7 0.2814846007770413 1107 8 0.05940843593231193 1107 9 -0.02176025284213523 1107 10-11 0.09890976240654226 1107 12-13 0.06337239262536087 1107 14-15 0.05161891972964128 1107 16-17 0.09313633110203057 1107 18-19 0.03941466648257119 1107 20-21 0.043639938950285284 1107 22-23 -0.05069611318863565 1107 24-25 -0.020154215154619237 1107 26-27 0.05063723904940076 1107 28-29 0.12121293886517748 1107 30-31 0.0671200512018757 1107 32-33 0.05030444540941659 1107 34-35 0.025082792499908635 1107 36-37 0.06513969791072327 1107 38-39 0.15901591981695873 1107 40-41 0.03472634043961165 1107 42-43 0.04979513942944891 1107 44-45 -0.013764984347034215 1107 46-47 -0.005241667335774025 1107 48-49 -0.08526689031290147 1107 50-51 -0.1529852897672015 1107 52-53 -0.08588401160101711 1107 54-55 0.05625922657856819 1107 56-57 0.0031655905425509445 1107 58-59 0.0721291536361548 1107 60-61 0.0645642975829901 1107 62-63 -0.016542248965883743 1107 64-65 0.05005045986256107 1107 66-67 0.18303478710262056 1107 68-69 0.24235129375104947 1107 70-71 0.161362928785735 1107 72-73 0.10927655876069053 1107 74-75 0.12095645060685456 1107 76-77 0.14530101612627533 1107 78-79 0.10338476453706846 1107 80-81 0.08889253841589806 1107 82-83 -0.0012134609789384854 1107 84-85 0.044549853806135076 1107 86-87 0.0177784365760445 1107 88-89 0.06093826456646312 1107 90-91 0.021987930217523655 1107 92-93 0.00893155869960438 1107 94-95 0.04452666517603632 1107 96-97 -0.024163126044051353 1107 98-99 0.15441954574643546 1107 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0.4651376664338862 2316 98-99 0.4701746243816629 2316 100 0.524007642805028 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 9945.0 1 805.0 2 550.0 3 447.0 4 379.0 5 444.0 6 446.0 7 735.0 8 1218.0 9 1553.0 10 2613.0 11 5171.0 12 8357.0 13 10899.0 14 12282.0 15 13332.0 16 14912.0 17 17552.0 18 21210.0 19 25175.0 20 29597.0 21 34598.0 22 40139.0 23 43723.0 24 45072.0 25 46267.0 26 48461.0 27 53434.0 28 60462.0 29 71578.0 30 85986.0 31 106831.0 32 139343.0 33 192126.0 34 302878.0 35 534658.0 36 653799.0 37 701343.0 38 768581.0 39 198684.0 40 325.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.60804332917321 17.678806260240798 12.226147044867634 23.487003365718355 2 16.1250687801513 20.903977673933806 39.45075922817328 23.520194317741613 3 20.23162374614254 26.037088983117023 29.632888669881385 24.098398600859056 4 12.926712098712024 15.91788946806187 37.7594420049149 33.3959564283112 5 13.016129216491331 37.87378551832221 33.94695268512956 15.163132580056892 6 30.402070102643208 35.834808306314805 18.802399589632156 14.960722001409824 7 27.392655304047004 32.75054706838448 22.20589715487161 17.650900472696907 8 26.216780760005143 33.65116699499953 20.748897118436528 19.383155126558805 9 27.666262639819152 16.168739692538832 20.373228537996 35.79176912964602 10-11 25.510890304492463 25.081942544364043 28.798084392842494 20.609082758301 12-13 26.312415828220033 23.295300870505976 28.021756708873795 22.3705265924002 14-15 23.26283048465084 24.88733593614156 26.926743289682353 24.923090289525245 16-17 22.60145653709383 27.380486688587897 27.237954046935968 22.780102727382303 18-19 22.26992461479179 27.457532626619336 28.376212679569303 21.896330079019577 20-21 23.46851349504847 26.10577249581485 28.000578716175013 22.425135292961667 22-23 22.79988929938297 25.94035538345813 28.343341504647817 22.91641381251108 24-25 23.089975684891854 25.780421317432957 28.281042765655428 22.848560232019764 26-27 22.63070387183377 25.507071330609822 29.02380404683355 22.838420750722857 28-29 22.322038655207834 27.43954247623588 28.138273040953475 22.10014582760281 30-31 23.497880023296446 25.849726266744323 28.27439720442633 22.377996505532906 32-33 23.376642739969654 25.965224576763273 28.335506316794778 22.322626366472292 34-35 23.58373894340058 26.252885184928488 28.068488451397556 22.09488742027338 36-37 23.093462307490505 26.153239676561217 28.565447519481026 22.187850496467252 38-39 22.86455188456962 26.838205783797896 28.157071914016008 22.140170417616474 40-41 22.98518771346171 26.535423996181535 28.010196869832242 22.46919142052451 42-43 22.546271147602745 26.871054710384712 27.223123044865705 23.359551097146838 44-45 22.55523925557168 26.403665300778766 27.847317183397823 23.19377826025173 46-47 22.927600906015194 26.405799988235078 27.638181003850388 23.028418101899337 48-49 22.86668209269177 26.49856726713984 27.709941327509657 22.92480931265873 50-51 22.236538370805466 26.81816672656538 28.391815641660845 22.55347926096831 52-53 22.674097336728213 27.24824769418112 27.21577104369257 22.861883925398093 54-55 22.143419731093978 26.31459657862118 28.046226358038812 23.495757332246033 56-57 22.915890481807004 26.356330236440716 27.40995274774976 23.31782653400252 58-59 23.297641056426766 26.544413219107483 27.469482275937878 22.68846344852787 60-61 23.169888078697905 26.601399627899823 28.229052472648775 21.999659820753497 62-63 22.72840091393925 26.730299360783206 27.914323322323924 22.626976402953616 64-65 22.410587173995165 27.07081365782359 27.4361530048736 23.082446163307644 66-67 22.66968526646557 26.404014153647772 27.18988390033634 23.736416679550317 68-69 22.05396693601469 26.58907622860881 27.816307205188707 23.540649630187797 70-71 22.78605403158973 26.30670469209924 27.754556717832244 23.152684558478786 72-73 22.456359622490833 26.502912155624514 27.81587046146251 23.22485776042214 74-75 22.093976118147904 27.486550901011555 27.244488095199248 23.17498488564129 76-77 22.227431738506727 26.28123418308519 27.333050279496245 24.158283798911842 78-79 22.222429380973526 26.434593450238452 27.733983770380682 23.608993398407335 80-81 22.720153757458377 26.365099145334803 27.341909314378736 23.572837782828078 82-83 22.655782447389942 26.268835259015148 27.817496248070135 23.257886045524774 84-85 22.39347017164806 26.923771665775153 27.387322819321447 23.295435343255335 86-87 22.047028178169697 27.140539370121868 27.274895937464823 23.537536514243616 88-89 21.700677846496177 26.73383839725636 27.16835836979386 24.397125386453602 90-91 22.671689208709555 25.869055509049815 27.388637467848202 24.07061781439242 92-93 22.823623749868478 26.381056481073994 27.419309547204584 23.376010221852937 94-95 22.444610710402383 26.17401053302613 27.37494799332916 24.006430763242324 96-97 21.556395662307857 26.085268038110637 28.185639815652046 24.172696483929464 98-99 21.97604580369204 26.435738515076636 27.20249748478958 24.38571819644175 100 21.797060810606038 25.736301026557516 27.465600741534757 25.001037421301685 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6641.0 1 5167.5 2 3397.5 3 2643.0 4 1764.0 5 1511.0 6 1924.0 7 3054.5 8 4758.5 9 5578.0 10 5096.0 11 4500.5 12 4548.5 13 4895.5 14 5214.0 15 5490.0 16 5758.0 17 6083.0 18 6472.5 19 6852.0 20 7396.5 21 8168.5 22 9255.5 23 10960.5 24 13252.0 25 16215.0 26 20142.5 27 24713.0 28 29742.0 29 35631.5 30 42512.5 31 48960.5 32 55281.0 33 62118.0 34 69162.5 35 75667.5 36 84094.0 37 92890.5 38 99858.5 39 106371.5 40 111448.5 41 116356.0 42 121388.0 43 127335.0 44 133746.0 45 141008.0 46 151079.0 47 174273.5 48 218094.0 49 224076.0 50 192679.5 51 178526.0 52 171194.5 53 163065.0 54 155490.0 55 144642.0 56 131589.5 57 119664.0 58 105752.0 59 88856.5 60 72869.5 61 59798.5 62 48377.5 63 37795.5 64 28231.0 65 21101.5 66 15813.5 67 12109.5 68 9501.0 69 7187.0 70 5430.5 71 4212.0 72 3276.0 73 2608.5 74 2009.0 75 1461.0 76 1028.5 77 667.5 78 468.5 79 336.0 80 224.5 81 150.0 82 113.5 83 95.0 84 78.0 85 62.5 86 47.5 87 38.5 88 26.5 89 21.5 90 20.5 91 14.0 92 11.5 93 11.5 94 7.0 95 3.5 96 4.0 97 6.0 98 5.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20341809280732762 2 0.18103025841227524 3 0.3680755055261257 4 0.3644525779684201 5 0.37429950927910705 6 0.3059980352585168 7 0.32378753852263514 8 0.3139406072119482 9 0.3278284961831529 10-11 0.3010513457085726 12-13 0.3100157690244339 14-15 0.29555889463551255 16-17 0.2904728617179644 18-19 0.314823115206774 20-21 0.3170177732465379 22-23 0.30773982735356753 24-25 0.31391738331734753 26-27 0.30301376480232983 28-29 0.32974446748770875 30-31 0.31143242659507514 32-33 0.311769173066785 34-35 0.31369675631864113 36-37 0.3201181631757282 38-39 0.3260983160354025 40-41 0.352573555880174 42-43 0.32154643269366984 44-45 0.3070198866209466 46-47 0.3132322784266276 48-49 0.33584073982038637 50-51 0.3237991504699355 52-53 0.3514936447812425 54-55 0.35194651072595573 56-57 0.3582634100573398 58-59 0.3455483277634693 60-61 0.32641183861251166 62-63 0.3491480314265742 64-65 0.3444335808226368 66-67 0.3319971852639744 68-69 0.3424479378342789 70-71 0.3628385172936731 72-73 0.3666820718500851 74-75 0.3550469006551461 76-77 0.33948689127269266 78-79 0.34955444958208604 80-81 0.36035356057140067 82-83 0.36657756432438204 84-85 0.35869305210745234 86-87 0.3590646344210632 88-89 0.3476152543829295 90-91 0.36042323225520273 92-93 0.3416583254178559 94-95 0.3621766362975538 96-97 0.3710946118242137 98-99 0.36028388888759866 100 0.38152214049991756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4305910.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.037087873684435 #Duplication Level Percentage of deduplicated Percentage of total 1 78.5817152280125 48.74980772865059 2 13.081106438612366 16.23027499234429 3 3.807568831003874 7.086314464622678 4 1.6322626096693735 4.050432757959536 5 0.8147813725133164 2.527333180222491 6 0.4815885027552796 1.7925808958631242 7 0.32239717930472295 1.4000407499928564 8 0.23955581607946635 1.1889076170219233 9 0.15216163199375943 0.8495698081500077 >10 0.8314962076319902 8.787051588519638 >50 0.03665706146341272 1.5415557527648234 >100 0.016418828111804593 1.9116575832845077 >500 0.0012723849487283427 0.5574919865740223 >1k 7.210180954053818E-4 1.1561353986607812 >5k 1.6965131656597045E-4 0.7000749784289323 >10k+ 1.2723848742447782E-4 1.4707705169399201 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 29279 0.6799724100132144 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15020 0.3488228969021647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11599 0.26937395347324955 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7649 0.1776395698005764 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7472 0.17352894045625664 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5964 0.1385073073984361 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5522 0.12824234598493697 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4504 0.10460042128144806 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.3223894600676745E-5 0.0 0.0 0.0 3 0.0 2.3223894600676745E-5 0.0 0.0 0.0 4 0.0 2.3223894600676745E-5 0.0 0.0 2.3223894600676745E-5 5 0.0 2.3223894600676745E-5 0.0 0.0 2.3223894600676745E-5 6 2.3223894600676745E-5 2.3223894600676745E-5 0.0 0.0 9.289557840270698E-5 7 2.3223894600676745E-5 2.3223894600676745E-5 0.0 0.0 9.289557840270698E-5 8 2.3223894600676745E-5 2.3223894600676745E-5 0.0 2.3223894600676745E-5 9.289557840270698E-5 9 2.3223894600676745E-5 2.3223894600676745E-5 0.0 2.3223894600676745E-5 1.1611947300338373E-4 10-11 2.3223894600676745E-5 4.644778920135349E-5 0.0 2.3223894600676745E-5 1.1611947300338373E-4 12-13 2.3223894600676745E-5 4.644778920135349E-5 0.0 4.644778920135349E-5 1.8579115680541396E-4 14-15 2.3223894600676745E-5 4.644778920135349E-5 0.0 9.289557840270698E-5 1.8579115680541396E-4 16-17 2.3223894600676745E-5 4.644778920135349E-5 0.0 1.3934336760406047E-4 1.8579115680541396E-4 18-19 2.3223894600676745E-5 4.644778920135349E-5 0.0 1.8579115680541396E-4 1.8579115680541396E-4 20-21 2.3223894600676745E-5 4.644778920135349E-5 0.0 3.599703663104896E-4 1.8579115680541396E-4 22-23 2.3223894600676745E-5 4.644778920135349E-5 0.0 5.92209312317257E-4 1.9740310410575233E-4 24-25 2.3223894600676745E-5 4.644778920135349E-5 0.0 0.0010218513624297768 2.090150514060907E-4 26-27 2.3223894600676745E-5 4.644778920135349E-5 0.0 0.0015792248328460187 2.090150514060907E-4 28-29 2.3223894600676745E-5 4.644778920135349E-5 0.0 0.003657763399606587 2.090150514060907E-4 30-31 2.3223894600676745E-5 4.644778920135349E-5 0.0 0.011565499511137018 2.2062699870642908E-4 32-33 2.3223894600676745E-5 4.644778920135349E-5 0.0 0.03124775018521056 2.554628406074442E-4 34-35 4.644778920135349E-5 4.644778920135349E-5 0.0 0.060254394541455814 2.554628406074442E-4 36-37 4.644778920135349E-5 4.644778920135349E-5 0.0 0.1039153163907281 2.554628406074442E-4 38-39 4.644778920135349E-5 4.644778920135349E-5 0.0 0.17301801477504175 2.7868673520812094E-4 40-41 4.644778920135349E-5 4.644778920135349E-5 0.0 0.257413647753901 2.7868673520812094E-4 42-43 4.644778920135349E-5 4.644778920135349E-5 0.0 0.33646778497460467 2.902986825084593E-4 44-45 4.644778920135349E-5 5.805973650169186E-5 0.0 0.4380490999579647 3.135225771091361E-4 46-47 4.644778920135349E-5 6.967168380203024E-5 0.0 0.556247111528109 3.2513452440947443E-4 48-49 4.644778920135349E-5 6.967168380203024E-5 0.0 0.6776267966585461 3.2513452440947443E-4 50-51 6.967168380203024E-5 6.967168380203024E-5 0.0 0.813416908388703 3.2513452440947443E-4 52-53 6.967168380203024E-5 6.967168380203024E-5 0.0 0.95710314428309 3.9480620821150467E-4 54-55 6.967168380203024E-5 1.1611947300338373E-4 0.0 1.1308062639488516 4.4125399741285816E-4 56-57 6.967168380203024E-5 1.1611947300338373E-4 0.0 1.3228794842437486 4.4125399741285816E-4 58-59 6.967168380203024E-5 1.1611947300338373E-4 0.0 1.542310452378243 4.5286594471319656E-4 60-61 6.967168380203024E-5 1.1611947300338373E-4 0.0 1.7733649797603759 4.644778920135349E-4 62-63 1.1611947300338373E-4 1.277314203037221E-4 0.0 2.0261338485941414 5.341495758155651E-4 64-65 1.1611947300338373E-4 1.6256726220473722E-4 0.0 2.2779505377492795 5.341495758155651E-4 66-67 1.1611947300338373E-4 1.6256726220473722E-4 0.0 2.567041577738504 5.457615231159035E-4 68-69 1.1611947300338373E-4 1.6256726220473722E-4 0.0 2.9150400263823446 5.573734704162419E-4 70-71 1.1611947300338373E-4 1.9740310410575233E-4 0.0 3.2839632040613944 5.573734704162419E-4 72-73 1.1611947300338373E-4 2.3223894600676745E-4 0.0 3.6249015887466296 7.896124164230093E-4 74-75 1.1611947300338373E-4 2.3223894600676745E-4 0.0 3.9882045839323164 7.896124164230093E-4 76-77 1.1611947300338373E-4 2.3223894600676745E-4 0.0 4.394390500498153 7.896124164230093E-4 78-79 1.3934336760406047E-4 2.554628406074442E-4 0.0 4.863838305956232 7.896124164230093E-4 80-81 1.3934336760406047E-4 2.554628406074442E-4 0.0 5.3257499576163925 7.896124164230093E-4 82-83 1.3934336760406047E-4 2.554628406074442E-4 0.0 5.799726422521604 8.592841002250395E-4 84-85 1.3934336760406047E-4 2.554628406074442E-4 0.0 6.306018936763657 8.592841002250395E-4 86-87 1.3934336760406047E-4 2.554628406074442E-4 0.0 6.820242411011842 8.941199421260547E-4 88 1.3934336760406047E-4 2.554628406074442E-4 0.0 7.228762328985046 9.754035732284233E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4355 0.0 40.373276 2 GTACCTG 5435 0.0 32.958416 1 ACCTGGG 5250 0.0 32.50709 3 GTACATG 16695 0.0 30.160875 1 TACATGG 16745 0.0 29.310677 2 ATGGGGG 8715 0.0 29.12942 5 CATGGGG 12605 0.0 28.008013 4 ACATGGG 17150 0.0 27.907082 3 TATCACG 365 0.0 25.760107 2 TGGGGGG 9230 0.0 25.703152 6 CCTGGGG 5040 0.0 24.437456 4 TATAACG 385 0.0 24.421919 2 GAGTACT 12940 0.0 20.022255 12-13 AGAGTAC 19745 0.0 19.521269 10-11 GAGTACA 12300 0.0 18.615074 1 ATAACGC 480 0.0 18.609863 3 CTGGGGG 3480 0.0 17.967094 5 AGTACTT 13500 0.0 17.625744 12-13 GGGTTAG 1530 0.0 17.515574 1 AGTACAT 12045 0.0 17.407587 2 >>END_MODULE