FastQCFastQC Report
Mon 6 Jun 2016
ERR522841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522841_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305910
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT300980.6989927796911687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139860.3248093898850649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116960.27162667124951523No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT76530.17773246537897913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71970.16714236944107053No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC62910.1461015209328574No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC56990.13235297532925677No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA46980.10910585683397934No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG45870.10652800453330423No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG42550.040.430192
GTACCTG52400.033.750371
GTACATG159350.030.8155821
ACCTGGG55900.030.0130443
TACATGG163150.029.6738852
ATAACGC4600.028.6057853
ACATGGG164050.028.4750063
TATAACG4800.027.4183352
ATGGGGG84600.027.3854665
CATGGGG122200.027.381834
TATCACG3850.025.6379222
TGGGGGG83100.025.3915316
TAACGCA4900.023.9771524
CCTGGGG49950.022.8625374
GAGTACT131800.019.92246212-13
AGAGTAC199400.018.9329410-11
GTACTTT146200.017.74776614-15
AGTACTT138200.017.7411412-13
GGTTAAG34900.017.3707263
GAGTACA117850.016.72271
Produced by FastQC (version 0.11.5)