FastQCFastQC Report
Mon 6 Jun 2016
ERR522840_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522840_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9123970
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269320.2951785242608207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225860.24754575036963078No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT135470.14847703357200867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126350.13848138474808663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG109500.041.150322
ACCTGGG123150.035.2518543
GTACCTG155500.029.642521
GTACATG320950.025.1037981
TACATGG331400.023.773012
CCTGGGG122150.023.6036574
GTATCAA457350.023.4274221
CATGGGG202750.023.2676434
ATGGGGG127900.023.238925
ACATGGG329900.022.9967633
GGTATCA345450.022.4247631
CCGTATC38950.022.07670294
TATAACG7500.021.9497012
ATAACGC8650.021.7499663
CGTATCA28400.020.84696294
TCAACGC510800.020.6165284
CAACGCA512450.020.5297625
GAGTACT253800.020.50942412-13
ATCAACG518800.020.271533
AACGCAG525800.020.160666
Produced by FastQC (version 0.11.5)