FastQCFastQC Report
Mon 6 Jun 2016
ERR522840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522840_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9123970
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265200.2906629460640489No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235490.25810036639752215No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT183060.2006363458012247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125820.13790049726160872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG109950.041.1731382
ACCTGGG126500.034.743653
TATAACG9000.032.3841172
GTACCTG157850.029.1457231
ATAACGC10650.026.9236873
TAACGCA10850.026.8604894
CCTGGGG115100.024.0950934
GTACATG321950.023.8604321
GGTATCA361550.023.1986941
TACATGG330200.022.8781912
GTATCAA474550.022.0560931
CATGGGG195400.022.0354984
ACATGGG325900.022.0246493
GAGTACT263650.021.1323212-13
ATGGGGG120000.020.9570065
TCAACGC524900.019.7014434
CAACGCA530600.019.5165925
ATCAACG534650.019.3774383
TGGGGGG123200.019.265296
GTACTTT286150.019.23258814-15
Produced by FastQC (version 0.11.5)