FastQCFastQC Report
Mon 6 Jun 2016
ERR522839_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522839_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6775036
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177920.26261115070089663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146820.21670733557725744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88130.13008048960920648No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91850.051.6355631
GTATCAA166750.041.0266571
TCAACGC203150.032.9188234
CAACGCA205600.032.5491685
ATCAACG206600.032.3485263
TACCTGG62400.032.1647262
TATCAAC214000.031.5191652
AACGCAG214450.031.3085386
GTACATG207600.029.259471
TACATGG217100.027.5399992
ACGCAGA244150.027.3652557
GTACCTG78850.026.3747371
ACATGGG220700.026.1292783
CGCAGAG258200.025.8022378
CATGGGG157400.024.602474
ACCTGGG83400.023.950143
GCAGAGT285450.022.9757799
ATGGGGG95300.020.71145
AGAGTAC258250.020.32943310-11
CAGAGTA277300.020.18655810-11
Produced by FastQC (version 0.11.5)