FastQCFastQC Report
Mon 6 Jun 2016
ERR522839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522839_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6775036
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168200.24826436346611294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147170.21722393799826303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86450.1276007979883797No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96400.047.924231
GTATCAA169450.040.285291
TCAACGC205150.032.6651344
TACCTGG62950.032.2753332
CAACGCA210700.031.7824085
ATCAACG211700.031.6334443
TATCAAC216950.031.1732442
AACGCAG220250.030.6603646
GTACATG208150.027.4612661
ACGCAGA250300.026.8284157
TACATGG210250.026.4625382
GTACCTG80350.025.9966751
CGCAGAG261600.025.6396718
ACATGGG214450.025.1356333
ACCTGGG80900.024.3979473
CATGGGG148500.022.974534
GCAGAGT298650.022.1913039
GAGTACT177150.020.17843412-13
AGAGTAC266300.019.6979410-11
ATGGGGG86000.019.398425
Produced by FastQC (version 0.11.5)