FastQCFastQC Report
Mon 6 Jun 2016
ERR522838_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522838_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9551007
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT905960.9485491948650022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293060.3068367555379239No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT271640.2844098009770069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230880.24173367269021998No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC195100.20427165428734373No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA158600.1660557886723358No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG151960.15910364216045492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134170.14047733396070175No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC119540.1251595774141931No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG383400.028.5095771
TACATGG392900.027.388322
ACATGGG407200.025.7931543
CATGGGG276100.025.0291274
ATGGGGG161850.022.4530495
GAGTACT260850.021.46946112-13
AGAGTAC445150.019.84615910-11
GTACTTT283150.019.53774314-15
AGTACTT276400.018.93576612-13
GAGTACA294800.016.4259191
ACTTTTT350400.016.082816-17
CATGGGA130300.016.0189254
ATGGGGA99550.015.8202035
AGTACAT285550.015.7390082
TACTTTT329250.015.6392114-15
TGGGGGG151900.015.1563086
GTATAGA33450.014.6169631
GTATAGG29250.014.4656041
ATGGGGC90100.014.4531825
GTATATG39000.014.34505751
Produced by FastQC (version 0.11.5)