##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522838_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9551007
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.948241688023053	34.0	31.0	34.0	30.0	34.0
2	32.25988191611628	34.0	31.0	34.0	30.0	35.0
3	32.111055933683225	34.0	31.0	34.0	28.0	35.0
4	34.72902061531313	35.0	35.0	37.0	32.0	37.0
5	34.74034266753233	37.0	34.0	37.0	32.0	37.0
6	34.912551734073695	37.0	35.0	37.0	33.0	37.0
7	34.76761089171016	37.0	35.0	37.0	32.0	37.0
8	34.90808173420876	37.0	35.0	37.0	33.0	37.0
9	36.16079613385269	39.0	35.0	39.0	32.0	39.0
10-11	36.263548597545785	38.0	35.5	39.0	33.0	39.0
12-13	36.31654463241415	38.0	36.5	39.0	33.0	39.0
14-15	37.399329725127416	39.0	37.0	41.0	32.5	41.0
16-17	37.29673101485529	39.0	36.5	41.0	33.0	41.0
18-19	37.329803182010025	39.0	36.5	41.0	32.5	41.0
20-21	37.35346000688723	39.0	36.0	41.0	33.0	41.0
22-23	37.260081842679	39.0	36.0	41.0	32.0	41.0
24-25	37.25806959412762	39.0	36.5	41.0	32.0	41.0
26-27	37.08186388094994	39.0	36.5	41.0	32.0	41.0
28-29	36.89779543664872	39.0	36.0	41.0	31.0	41.0
30-31	36.81975795850636	38.5	36.0	41.0	31.5	41.0
32-33	36.65311992756366	38.0	35.0	41.0	30.5	41.0
34-35	36.54768732762943	38.5	35.5	41.0	30.5	41.0
36-37	36.43267694181357	38.0	35.5	40.5	30.5	41.0
38-39	36.19486672975949	38.0	35.5	40.0	30.0	41.0
40-41	36.162567674801195	38.0	35.0	40.0	30.0	41.0
42-43	36.117730046685125	38.0	35.0	40.0	29.0	41.0
44-45	36.01197182663566	38.0	35.0	40.0	29.0	41.0
46-47	35.72507171233358	38.0	35.0	40.0	28.0	41.0
48-49	35.83397933851373	38.0	35.5	40.0	28.0	41.0
50-51	34.815173049292085	37.5	34.0	39.0	26.5	40.0
52-53	34.976506927489424	37.0	34.5	39.5	27.5	40.0
54-55	35.56626453105939	37.0	35.0	40.0	28.0	41.0
56-57	35.7103957729274	37.5	35.0	40.0	28.0	41.0
58-59	35.701455877898525	38.0	35.0	40.0	28.0	41.0
60-61	35.42638535392132	37.0	35.0	40.0	28.0	41.0
62-63	35.229588879999774	37.5	34.0	40.0	28.0	41.0
64-65	35.00181839464676	37.0	35.0	39.5	27.5	41.0
66-67	34.755206911690046	37.0	34.0	39.0	26.0	41.0
68-69	34.41106304288124	36.5	34.0	39.0	26.0	41.0
70-71	34.001262746430825	36.0	34.0	38.0	26.0	40.5
72-73	33.74356646372472	36.0	34.0	37.5	26.0	40.0
74-75	33.48880154731329	35.0	33.5	37.0	25.0	39.0
76-77	33.15058474985936	35.0	33.0	37.0	25.5	39.0
78-79	32.84860643490262	35.0	33.0	37.0	24.5	38.5
80-81	32.61608048240359	35.0	33.0	36.0	24.0	38.5
82-83	32.38694181671106	35.0	32.5	36.0	23.0	38.0
84-85	32.095644940894715	35.0	32.0	36.0	22.0	37.0
86-87	32.02149270752288	35.0	32.0	36.0	22.5	37.0
88-89	31.713025862089726	35.0	32.0	35.5	20.0	37.0
90-91	31.612103519555582	35.0	31.5	35.5	20.0	36.5
92-93	31.37154590086679	35.0	31.5	35.0	19.5	36.0
94-95	31.279632346620623	34.5	31.0	35.0	19.0	36.0
96-97	31.019756974316948	34.5	30.5	35.0	19.0	36.0
98-99	30.983602671425118	34.5	31.0	35.0	18.5	36.5
100	29.73569938750961	34.0	29.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.026894059843371565
1101	2	-0.032452234519979584
1101	3	-0.051339279958973805
1101	4	-0.0075839053200184026
1101	5	0.0027022418694357953
1101	6	-0.056306439984965095
1101	7	0.07999627583284763
1101	8	0.042145710995733054
1101	9	-0.12139027979998929
1101	10-11	0.022959973457048477
1101	12-13	-0.0379686285470342
1101	14-15	3.6730473346580084E-4
1101	16-17	-0.13047152628735859
1101	18-19	-0.09474076429494716
1101	20-21	-0.04834636585685814
1101	22-23	-0.07632460196334279
1101	24-25	-0.11070346080335725
1101	26-27	-0.20055353571393653
1101	28-29	-0.19482609833713838
1101	30-31	-0.19497967561794383
1101	32-33	-0.15270080635648497
1101	34-35	-0.316201599251805
1101	36-37	-0.23923643670075023
1101	38-39	-0.2564955526161228
1101	40-41	-0.2910038532966013
1101	42-43	-0.25390450846681745
1101	44-45	-0.3091338376468258
1101	46-47	-0.3952306485243895
1101	48-49	-0.3486858151308567
1101	50-51	-0.5278458953885092
1101	52-53	-0.46805162169793135
1101	54-55	-0.37224169173885713
1101	56-57	-0.29655720573340716
1101	58-59	-0.30614338881989767
1101	60-61	-0.23176131066158234
1101	62-63	-0.24447348282312475
1101	64-65	-0.2251959668853445
1101	66-67	-0.5796381465112788
1101	68-69	-0.33296836641980576
1101	70-71	-0.3346382427264203
1101	72-73	-0.3807259395114997
1101	74-75	-0.44912705155316957
1101	76-77	-0.3724251999787853
1101	78-79	-0.6604356453706401
1101	80-81	-0.3851469061185284
1101	82-83	-0.39557094388022307
1101	84-85	-0.7859617880133953
1101	86-87	-0.9786870420127727
1101	88-89	-0.5193815715568242
1101	90-91	-0.746226431659359
1101	92-93	-0.5614493493042829
1101	94-95	-0.7071845697909183
1101	96-97	-0.6501190543436799
1101	98-99	-0.6989696916243027
1101	100	-0.7332831140650065
1102	1	-0.0454421910699736
1102	2	-0.05142975001376726
1102	3	0.04353349115594085
1102	4	-0.007265568708135106
1102	5	0.02263688239231243
1102	6	0.049263921924634246
1102	7	0.20325917322481502
1102	8	0.06463021720382756
1102	9	0.01519516343879701
1102	10-11	0.0919253265885942
1102	12-13	-0.008757278419061265
1102	14-15	0.02980709575235352
1102	16-17	-0.007275786286932373
1102	18-19	-0.06239681269057229
1102	20-21	-0.0027977904396365716
1102	22-23	-0.07259075675319338
1102	24-25	-0.01251142239144798
1102	26-27	-0.07484335951832577
1102	28-29	-0.35656647949499387
1102	30-31	-0.17724589386360634
1102	32-33	-0.1146389581004641
1102	34-35	-0.11977733375272948
1102	36-37	-0.1883512282484361
1102	38-39	-0.2684395211420494
1102	40-41	-0.6818223273960626
1102	42-43	-0.17153200175107486
1102	44-45	-0.19894177808298252
1102	46-47	-0.22781678977700182
1102	48-49	-0.4851099748702623
1102	50-51	-0.3856752838860871
1102	52-53	-0.4208457922248314
1102	54-55	-0.1985129229929541
1102	56-57	-0.07361317375168142
1102	58-59	-0.10497844603587936
1102	60-61	-0.5734618204231978
1102	62-63	-0.2071360883183999
1102	64-65	-0.18801509669476246
1102	66-67	-0.16126716273481634
1102	68-69	-0.3633504761137374
1102	70-71	-0.06030082706976003
1102	72-73	-0.3304721162993687
1102	74-75	-0.1491085436106232
1102	76-77	-0.38046875181160544
1102	78-79	-0.19088491245612005
1102	80-81	-0.2853859229766229
1102	82-83	-0.283956945530079
1102	84-85	-0.2711776435033819
1102	86-87	-0.34895271030814
1102	88-89	-0.1901966613467856
1102	90-91	-0.2823031708771637
1102	92-93	-0.34840495139409455
1102	94-95	-0.44898872403082635
1102	96-97	-0.37939756186319684
1102	98-99	-0.4574970939552472
1102	100	-0.7098118701197471
1103	1	-0.04349208333924892
1103	2	-0.0411834828242732
1103	3	0.0021346234096029093
1103	4	-0.04462123346464608
1103	5	0.007119333844300968
1103	6	-0.028025046068648862
1103	7	0.06241593886998231
1103	8	-0.07708287716737061
1103	9	-0.1450665844568917
1103	10-11	-0.08729711530398987
1103	12-13	-0.10788000957311539
1103	14-15	-0.1541149080102926
1103	16-17	-0.13337040482446127
1103	18-19	-0.25595582992806243
1103	20-21	-0.2440157328375676
1103	22-23	-0.291970150520676
1103	24-25	-0.24125214621968638
1103	26-27	-0.3559180664659607
1103	28-29	-0.4198582657263401
1103	30-31	-0.2954712399020707
1103	32-33	-0.42355616197934154
1103	34-35	-0.48056793370648876
1103	36-37	-0.49104054690709376
1103	38-39	-0.3682820042357875
1103	40-41	-0.31852362241335186
1103	42-43	-0.26695005865435206
1103	44-45	-0.2970113023092722
1103	46-47	-0.3710224029599871
1103	48-49	-0.2929332088445662
1103	50-51	-0.3882695850258955
1103	52-53	-0.24339201380822573
1103	54-55	-0.21480479513212458
1103	56-57	-0.12826222873673032
1103	58-59	-0.09497012623734946
1103	60-61	-0.054284800133380884
1103	62-63	-0.07054321590074863
1103	64-65	-0.07745065342650292
1103	66-67	-0.10219907241973658
1103	68-69	-0.0958698183480422
1103	70-71	-0.06531836322167806
1103	72-73	-0.09606917283191052
1103	74-75	-0.13357681162229085
1103	76-77	-0.34712221519440334
1103	78-79	-0.24265112554097357
1103	80-81	-0.2688813857171297
1103	82-83	-0.19709917141923938
1103	84-85	-0.3048214846327042
1103	86-87	-0.43133978907869874
1103	88-89	-0.2552474536303002
1103	90-91	-0.32651384800958994
1103	92-93	-0.3540899164284568
1103	94-95	-0.48690342389826924
1103	96-97	-0.478240746633638
1103	98-99	-0.4625436312066675
1103	100	-0.4405920688839444
1104	1	-0.03056327901033029
1104	2	-0.013540481784694691
1104	3	-0.0038380506704172035
1104	4	-0.032337264915007324
1104	5	-0.044959623665732806
1104	6	-0.038145243882588886
1104	7	0.08243330587168884
1104	8	-0.019035243963500648
1104	9	-0.036030830300269656
1104	10-11	-0.1056399773714034
1104	12-13	-0.06697898679947656
1104	14-15	-0.023659945877497535
1104	16-17	-0.08606704523129594
1104	18-19	-0.11241913084209187
1104	20-21	-0.16548637768012497
1104	22-23	-0.06541070649061709
1104	24-25	-0.06630807279937301
1104	26-27	-0.10723303854729238
1104	28-29	-0.25112090751656524
1104	30-31	-0.09391790640007258
1104	32-33	-0.08269321423350817
1104	34-35	-0.1226421044479693
1104	36-37	-0.2575238996149096
1104	38-39	-0.26391976490459257
1104	40-41	-0.015039943735409622
1104	42-43	-0.11175360800143608
1104	44-45	-0.11256857117206209
1104	46-47	-0.18620097156485116
1104	48-49	-0.20604957588827943
1104	50-51	-0.2729997820582284
1104	52-53	-0.1489149598010968
1104	54-55	-0.16684198418982987
1104	56-57	-0.7239742080372906
1104	58-59	-0.03527492637903151
1104	60-61	-0.02844006763444895
1104	62-63	-0.03487052921564526
1104	64-65	-0.09282143258773345
1104	66-67	-0.12507005037078756
1104	68-69	-0.15544109647171922
1104	70-71	-0.2162250424693326
1104	72-73	-0.13282484613529988
1104	74-75	-0.08911516382634943
1104	76-77	-0.17440474590630117
1104	78-79	-0.15957175938232382
1104	80-81	-0.19761668035300772
1104	82-83	-0.05820602749946602
1104	84-85	-0.1750116124139005
1104	86-87	-0.1956178906178323
1104	88-89	-0.20739400532717767
1104	90-91	-0.3806052587983686
1104	92-93	-0.5413677236231109
1104	94-95	-0.34812445988239205
1104	96-97	-0.3160237002257027
1104	98-99	-0.3683447756487084
1104	100	-0.3199457015194511
1105	1	0.013621616878491949
1105	2	-0.04642226321021781
1105	3	0.022681400826222387
1105	4	0.05031008708125029
1105	5	-0.0038264375257810457
1105	6	-0.01693041232567083
1105	7	0.1332400911871332
1105	8	0.030061429896910852
1105	9	-0.031601711686164435
1105	10-11	-0.09579557296118679
1105	12-13	-0.03456801367047291
1105	14-15	-0.040574353061238355
1105	16-17	-0.08065107679616546
1105	18-19	-0.12546865972716859
1105	20-21	-0.09736417392620922
1105	22-23	-0.05807689171400199
1105	24-25	-0.08074166907779556
1105	26-27	-0.1691473678430384
1105	28-29	-0.1933343396858831
1105	30-31	-0.11717160164816676
1105	32-33	-0.14025954705015664
1105	34-35	-0.30090885220170804
1105	36-37	-0.212795544732856
1105	38-39	-0.1020447466635801
1105	40-41	-0.30336759542316827
1105	42-43	-0.2968386773566678
1105	44-45	-0.36580043047662514
1105	46-47	-0.1876619421602541
1105	48-49	-0.25417878239942837
1105	50-51	-0.3346141754369967
1105	52-53	-0.3052380861069466
1105	54-55	-0.29558183883114
1105	56-57	-0.23075536498016191
1105	58-59	-0.22899007485951728
1105	60-61	-0.27859660459463953
1105	62-63	-0.1963200507237417
1105	64-65	-0.2634697946211517
1105	66-67	-0.20839812775671618
1105	68-69	-0.13246852539982257
1105	70-71	-0.2625075090047986
1105	72-73	-0.19973971885647046
1105	74-75	-0.2560300723580937
1105	76-77	-0.15584525702700347
1105	78-79	-0.1461217675919002
1105	80-81	-0.20408299522856055
1105	82-83	-0.20289003546517392
1105	84-85	-0.18116426025650156
1105	86-87	-0.12260080700569631
1105	88-89	-0.12978713673424735
1105	90-91	-0.11383454153232009
1105	92-93	-0.1373684683934684
1105	94-95	-0.19083495173521925
1105	96-97	-0.2040740572302404
1105	98-99	-0.19716551070365895
1105	100	-0.22113697151092637
1106	1	0.06801440423323157
1106	2	0.02263771495212552
1106	3	0.08505421369162747
1106	4	0.10062614222025701
1106	5	0.10523140420231414
1106	6	0.10919520401861149
1106	7	0.23981565661659232
1106	8	0.055558797667153215
1106	9	0.045111476992168775
1106	10-11	0.06831993346458631
1106	12-13	0.11217624224750722
1106	14-15	0.0712682388406094
1106	16-17	0.05994289763511773
1106	18-19	0.03559840856872398
1106	20-21	0.04078632701335039
1106	22-23	-0.019274777202518578
1106	24-25	0.021597573308120843
1106	26-27	-0.005398430571588619
1106	28-29	0.009970369226415698
1106	30-31	0.04744442889978018
1106	32-33	0.04086151327152976
1106	34-35	0.03884501809822183
1106	36-37	-0.009313996798880453
1106	38-39	0.006083054887255912
1106	40-41	0.035885807047648655
1106	42-43	0.06639142028230083
1106	44-45	0.025146248128081083
1106	46-47	0.05035987959714561
1106	48-49	-0.07331404127219088
1106	50-51	-0.02234503187264636
1106	52-53	0.06445048063951475
1106	54-55	0.09321572269757183
1106	56-57	0.10132958903977851
1106	58-59	0.1631323012111423
1106	60-61	0.12544317573002672
1106	62-63	0.1485148425165832
1106	64-65	0.16551170155698713
1106	66-67	0.06824744394852189
1106	68-69	0.13896828878041134
1106	70-71	0.1306494040521642
1106	72-73	0.08697673774663883
1106	74-75	0.1253522913456493
1106	76-77	0.0744022622109739
1106	78-79	0.10978618915530802
1106	80-81	0.14369041268151506
1106	82-83	0.13732503262454543
1106	84-85	0.11467441369334352
1106	86-87	0.0649272367899485
1106	88-89	0.11272810410536849
1106	90-91	0.15772402890945258
1106	92-93	0.012081232033999356
1106	94-95	0.029588797883874918
1106	96-97	0.005538705730689486
1106	98-99	0.0341632856491465
1106	100	-0.08042259185156198
1107	1	-0.04074789451848915
1107	2	-0.006432011906106538
1107	3	0.011661688253759905
1107	4	0.10470506148987369
1107	5	0.09384473289863138
1107	6	0.059052695978984104
1107	7	0.23987747019387484
1107	8	0.04628453045851444
1107	9	-0.008965326521881423
1107	10-11	0.0609704600402452
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2316	94-95	0.2684832290213741
2316	96-97	0.44151330329735927
2316	98-99	0.4056363864519703
2316	100	0.298167870504809
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	22026.0
1	1804.0
2	1222.0
3	1083.0
4	864.0
5	1018.0
6	1088.0
7	1568.0
8	2512.0
9	3332.0
10	4788.0
11	7913.0
12	12696.0
13	16917.0
14	20196.0
15	23576.0
16	26678.0
17	31609.0
18	37964.0
19	46259.0
20	56066.0
21	68830.0
22	80361.0
23	88127.0
24	90767.0
25	93651.0
26	99350.0
27	108622.0
28	125758.0
29	148349.0
30	180238.0
31	226513.0
32	296609.0
33	413805.0
34	654564.0
35	1203898.0
36	1505167.0
37	1585653.0
38	1791079.0
39	467981.0
40	506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.076371413109825	18.510612135871092	13.41221468390001	23.000801767119068
2	15.389586407730127	21.215983741067095	39.374847973711816	24.019581877490964
3	20.159120252564623	25.54614829911699	29.937140480349466	24.357590967968925
4	12.656889684821431	16.428305489997488	39.07956160052357	31.835243224657507
5	13.122611635497572	38.498231397727054	34.48996709560058	13.889189871174795
6	30.268309166516573	37.06062962727632	17.91470294323524	14.756358262971863
7	26.95866553220097	32.97069296946397	22.293280391600753	17.777361106734315
8	25.634616000668004	33.29411091265913	20.618596006791364	20.452677079881504
9	26.328496757168594	16.644815288184507	21.003889548871367	36.02279840577553
10-11	25.03788146306436	25.41447232829814	28.426033545514333	21.12161266312317
12-13	26.597088356513847	23.531469985689014	27.903388361198054	21.968053296599077
14-15	23.261917188495985	25.078828343969445	26.968225031215248	24.69102943631932
16-17	22.182711357486976	28.025229969298927	27.102045033775173	22.690013639438927
18-19	21.939122138717465	28.18282127393042	28.300571330922768	21.577485256429345
20-21	23.365382595507082	26.17072586642243	27.957122120502987	22.506769417567504
22-23	22.77405824167584	26.092018293760077	28.156346826938368	22.977576637625717
24-25	23.374830297934125	26.163815349868262	27.942617542640562	22.518736809557048
26-27	22.774268251910602	25.81097562928737	29.122033568469202	22.292722550332826
28-29	22.088647948631984	27.60630600597334	28.168732600452472	22.136313444942203
30-31	22.984623769181823	26.11764652749905	27.99035495320343	22.907374750115704
32-33	22.905100690391535	26.429402244456483	28.15015326600668	22.515343799145306
34-35	23.62674683172176	26.277333853725594	28.01347082136027	22.08244849319238
36-37	23.12326849074629	25.966811743617672	28.774814907405123	22.135104858230918
38-39	22.704644913788936	26.914590437053427	28.312739110430414	22.068025538727223
40-41	22.802554329409723	26.89240767349555	27.89252062718191	22.412517369912816
42-43	22.302826982360298	26.996955709363206	27.21936896567227	23.48084834260423
44-45	22.817589805753357	26.18469510290596	27.595449759210787	23.402265332129897
46-47	23.48140150287809	26.741819323783634	27.47971471678851	22.297064456549766
48-49	23.049349524040444	26.720775646200245	27.597875001208127	22.631999828551187
50-51	22.00600142953205	27.014594745941885	28.404079098112234	22.575324726413836
52-53	22.84458009992701	27.488575608501893	26.938117027860947	22.728727263710148
54-55	22.228129648486306	26.48492294643883	27.656894441028996	23.630052964045873
56-57	23.01872539457472	26.38300011915893	27.283727072677067	23.31454741358929
58-59	23.864223291076467	26.37568580728264	27.09747761604878	22.662613285592112
60-61	23.113076412591806	26.746494929804054	28.220600597556462	21.919828060047674
62-63	22.442586428801718	26.838503781691024	27.993423484094016	22.72548630541324
64-65	22.200569980168893	27.508388164374924	27.417606345954322	22.873435509501856
66-67	22.741872876989202	26.56950344067598	26.945333144942254	23.743290537392568
68-69	22.225564355299728	26.757038038470693	27.445355773976736	23.572041832252843
70-71	23.21836564513467	26.467413346021722	27.204857680415355	23.10936332842825
72-73	22.758228861455674	26.125141577968293	28.075097641246423	23.041531919329614
74-75	22.033030016957426	27.872190292170263	27.159234556474075	22.93554513439824
76-77	22.3375323520162	26.448239111623657	27.10080812343624	24.1134204129239
78-79	22.47924008686469	26.511654271947705	27.506509939547012	23.502595701640594
80-81	23.11976445543191	26.529752191995364	27.061948875009257	23.28853447756347
82-83	22.724623358966152	26.176313437891725	27.953569310520017	23.145493892622106
84-85	22.320670075411908	26.90919935117587	27.549039176331924	23.22109139708029
86-87	22.17880627757747	27.18199491604198	27.263247665842037	23.37595114053852
88-89	21.899832744979644	26.95147229911615	26.84122099211782	24.307473963786382
90-91	22.59781599647059	26.011669812308604	27.179092377003546	24.211421814217264
92-93	23.141002282056462	26.50338342462067	27.22168253586203	23.133931757460836
94-95	22.632020844027597	26.249603972933027	27.400743663960984	23.71763151907839
96-97	21.558734683359766	26.314449273540724	28.3418955326212	23.784920510478308
98-99	22.149850927731542	26.70910587375474	27.064514968043184	24.076528230470533
100	22.095907256369294	25.32275287984529	27.23346716555957	25.34787269822585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13952.0
1	10653.0
2	5351.0
3	2637.5
4	1750.5
5	1703.5
6	2249.0
7	3785.0
8	6250.5
9	7946.5
10	7682.0
11	7100.5
12	7484.5
13	8131.0
14	8802.5
15	9496.5
16	10108.5
17	10672.5
18	11429.5
19	12217.5
20	13238.5
21	14841.0
22	16935.0
23	20008.0
24	24260.0
25	30057.0
26	38420.5
27	48826.5
28	60400.5
29	74984.5
30	93087.5
31	109375.5
32	126725.0
33	146816.5
34	167388.0
35	187278.0
36	209248.0
37	230443.5
38	244728.0
39	258679.5
40	267997.5
41	275858.5
42	286207.5
43	297809.0
44	311833.0
45	326340.0
46	344499.0
47	414054.0
48	548571.5
49	542203.0
50	415369.5
51	357120.0
52	337655.0
53	325306.5
54	312004.5
55	293547.5
56	268625.5
57	241405.0
58	215478.0
59	185718.0
60	155502.5
61	130494.0
62	107355.5
63	83587.0
64	61825.5
65	46229.5
66	34570.5
67	26305.5
68	19909.5
69	14764.5
70	11473.0
71	8645.5
72	6345.0
73	4905.5
74	3795.5
75	2762.5
76	1979.5
77	1317.0
78	878.0
79	634.5
80	449.5
81	309.0
82	244.0
83	193.0
84	142.0
85	124.0
86	97.0
87	72.5
88	64.0
89	54.5
90	43.5
91	34.5
92	29.0
93	21.5
94	21.0
95	16.0
96	12.5
97	10.5
98	7.5
99	7.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20480562939593697
2	0.1810699123139581
3	0.36839047442850786
4	0.36484111047138795
5	0.3755206126432532
6	0.3072136791439897
7	0.32569340594138396
8	0.3150662542703612
9	0.32869832468974214
10-11	0.3022665568143757
12-13	0.3104279998957178
14-15	0.29752883648813155
16-17	0.29199015349899754
18-19	0.31402447930359595
20-21	0.316689119796478
22-23	0.3089621858721284
24-25	0.31315022593952657
26-27	0.3043186964474008
28-29	0.3302531345647637
30-31	0.31253772507966965
32-33	0.3121712715737723
34-35	0.3139354834521637
36-37	0.32218592238493804
38-39	0.32820622998182286
40-41	0.35422442890053374
42-43	0.323431864304989
44-45	0.30783141505393097
46-47	0.31349573924508695
48-49	0.3364147885139232
50-51	0.32570911109163675
52-53	0.3534758167384863
54-55	0.3538475052944679
56-57	0.35917678628023203
58-59	0.345795998264895
60-61	0.3288082607415113
62-63	0.35080594119552
64-65	0.346895358782587
66-67	0.3329701255584882
68-69	0.34342975562681505
70-71	0.36326536039602947
72-73	0.36696654080559254
74-75	0.35531855436814147
76-77	0.34000079782163284
78-79	0.3501777351854103
80-81	0.36128651146418383
82-83	0.36662626255011643
84-85	0.3602395014473343
86-87	0.36023426639725004
88-89	0.3476701461950557
90-91	0.36018715094649184
92-93	0.34151896234606466
94-95	0.3623911070319601
96-97	0.37209688988815526
98-99	0.36278373578827866
100	0.38327895686810826
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9551007.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.6563739416316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.96171233231698	35.237013231120216
2	15.373211774553116	15.26755905122207
3	5.719937390207411	8.520940499125787
4	2.764735883845608	5.491470355923394
5	1.4798188361745048	3.6741218747475637
6	0.9081430415795613	2.705705427909923
7	0.5859597388068006	2.036764513345189
8	0.4123224183519036	1.637954895215999
9	0.28918175496908544	1.292374562565797
>10	1.379424368043381	12.0536519829044
>50	0.08007501553333157	2.7252670348224486
>100	0.041836974846746325	3.782123711312336
>500	0.002141484388528836	0.722123467810928
>1k	0.0011930707954699359	1.0041481215241332
>5k	3.059155876346584E-5	0.110496852214204
>10k+	2.7532402887119257E-4	3.7382844182355885
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	90596	0.9485491948650022	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29306	0.3068367555379239	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	27164	0.2844098009770069	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23088	0.24173367269021998	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	19510	0.20427165428734373	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	15860	0.1660557886723358	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	15196	0.15910364216045492	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13417	0.14047733396070175	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	11954	0.1251595774141931	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0470100168495323E-5	1.0470100168495323E-5
5	0.0	0.0	0.0	1.0470100168495323E-5	1.0470100168495323E-5
6	0.0	0.0	0.0	1.0470100168495323E-5	1.0470100168495323E-5
7	0.0	0.0	0.0	1.0470100168495323E-5	1.0470100168495323E-5
8	0.0	0.0	0.0	2.0940200336990645E-5	2.0940200336990645E-5
9	0.0	1.0470100168495323E-5	0.0	2.0940200336990645E-5	5.235050084247661E-5
10-11	0.0	1.0470100168495323E-5	0.0	5.235050084247661E-5	5.235050084247661E-5
12-13	0.0	1.0470100168495323E-5	0.0	6.80556510952196E-5	6.282060101097193E-5
14-15	0.0	1.0470100168495323E-5	0.0	7.329070117946726E-5	1.0993605176920088E-4
16-17	0.0	1.0470100168495323E-5	0.0	1.1517110185344854E-4	1.361113021904392E-4
18-19	0.0	1.5705150252742983E-5	0.0	1.6752160269592516E-4	1.5705150252742984E-4
20-21	0.0	2.0940200336990645E-5	0.0	2.3034220370689708E-4	1.5705150252742984E-4
22-23	0.0	2.0940200336990645E-5	0.0	4.3974420707680356E-4	1.9369685311716346E-4
24-25	0.0	2.0940200336990645E-5	0.0	7.433771119631678E-4	2.408123038753924E-4
26-27	0.0	2.0940200336990645E-5	0.0	0.0013506429217358966	2.408123038753924E-4
28-29	0.0	2.0940200336990645E-5	0.0	0.003120089850211606	2.408123038753924E-4
30-31	0.0	3.1410300505485966E-5	0.0	0.008831529492125803	2.460473539596401E-4
32-33	0.0	3.1410300505485966E-5	0.0	0.024562854995290026	3.08867954970612E-4
34-35	0.0	3.1410300505485966E-5	0.0	0.04941363774521368	3.4027825547609797E-4
36-37	0.0	3.1410300505485966E-5	0.0	0.08665578404455154	3.455133055603456E-4
38-39	0.0	3.1410300505485966E-5	0.0	0.14079667201584084	3.5598340572884097E-4
40-41	0.0	3.1410300505485966E-5	0.0	0.19872773624812545	3.5598340572884097E-4
42-43	0.0	3.1410300505485966E-5	0.0	0.2592763255225339	3.612184558130886E-4
44-45	0.0	3.1410300505485966E-5	0.0	0.33857686419871746	3.7692360606583156E-4
46-47	0.0	3.1410300505485966E-5	0.0	0.43384954068194065	4.030988564870699E-4
48-49	0.0	3.1410300505485966E-5	0.0	0.5340641044446937	4.920947079192802E-4
50-51	0.0	3.1410300505485966E-5	0.0	0.6431259028498252	4.973297580035278E-4
52-53	0.0	3.1410300505485966E-5	0.0	0.7539780883837695	5.496802588460044E-4
54-55	0.0	3.1410300505485966E-5	0.0	0.8812264507815772	6.02030759688481E-4
56-57	0.0	3.1410300505485966E-5	0.0	1.0398118229836917	6.072658097727286E-4
58-59	0.0	3.1410300505485966E-5	0.0	1.2088777654544698	6.125008598569764E-4
60-61	0.0	3.1410300505485966E-5	0.0	1.3900419086699443	6.282060101097193E-4
62-63	0.0	3.1410300505485966E-5	0.0	1.5803830946831052	6.491462104467099E-4
64-65	0.0	3.1410300505485966E-5	0.0	1.7773937345035975	6.491462104467099E-4
66-67	0.0	3.1410300505485966E-5	0.0	2.0096833768418345	6.700864107837005E-4
68-69	0.0	3.1410300505485966E-5	0.0	2.279309396380926	6.910266111206912E-4
70-71	1.0470100168495323E-5	3.1410300505485966E-5	0.0	2.5713414302805973	6.910266111206912E-4
72-73	1.0470100168495323E-5	3.1410300505485966E-5	0.0	2.865655946017001	7.538472121316632E-4
74-75	1.0470100168495323E-5	3.1410300505485966E-5	0.0	3.171157763783442	7.538472121316632E-4
76-77	1.0470100168495323E-5	3.1410300505485966E-5	0.0	3.4949037310934856	7.538472121316632E-4
78-79	1.0470100168495323E-5	3.1410300505485966E-5	0.0	3.8937517269121464	7.538472121316632E-4
80-81	1.0470100168495323E-5	4.188040067398129E-5	0.0	4.316016101757647	7.904925627213969E-4
82-83	1.0470100168495323E-5	4.188040067398129E-5	0.0	4.735840943263888	8.585482138166164E-4
84-85	1.0470100168495323E-5	4.188040067398129E-5	0.0	5.165806076783317	8.585482138166164E-4
86-87	1.5705150252742983E-5	5.235050084247661E-5	0.0	5.61234014381939	8.79488414153607E-4
88	2.0940200336990645E-5	5.235050084247661E-5	0.0	5.976186594774771	8.79488414153607E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	38340	0.0	28.509577	1
TACATGG	39290	0.0	27.38832	2
ACATGGG	40720	0.0	25.793154	3
CATGGGG	27610	0.0	25.029127	4
ATGGGGG	16185	0.0	22.453049	5
GAGTACT	26085	0.0	21.469461	12-13
AGAGTAC	44515	0.0	19.846159	10-11
GTACTTT	28315	0.0	19.537743	14-15
AGTACTT	27640	0.0	18.935766	12-13
GAGTACA	29480	0.0	16.425919	1
ACTTTTT	35040	0.0	16.0828	16-17
CATGGGA	13030	0.0	16.018925	4
ATGGGGA	9955	0.0	15.820203	5
AGTACAT	28555	0.0	15.739008	2
TACTTTT	32925	0.0	15.63921	14-15
TGGGGGG	15190	0.0	15.156308	6
GTATAGA	3345	0.0	14.616963	1
GTATAGG	2925	0.0	14.465604	1
ATGGGGC	9010	0.0	14.453182	5
GTATATG	3900	0.0	14.3450575	1
>>END_MODULE